Browsing by Author "Koc K., Ozek N.S., Aysin F., Demir O., Yilmaz A., Yilmaz M., Geyikoglu F., Erol H.S."

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    Hispidulin exerts a protective effect against oleic acid induced-ARDS in the rat via inhibition of ACE activity and MAPK pathway.
    (2023-01-09T00:00:00Z) Koc, Kubra, Ozek, Nihal Simsek, Aysin, Ferhunde, Demir, Ozlem, Yilmaz, Asli, Yilmaz, Mehmet, Geyikoglu, Fatime, Erol, Huseyin Serkan; Koc, K, Ozek, NS, Aysin, F, Demir, O, Yilmaz, A, Yilmaz, M, Geyikoglu, F, Erol, HS
    This study investigates the protective role of Hispidulin on acute respiratory distress syndrome (ARDS) in rats. Rats were divided into three groups: control, ARDS, ARDS+ Hispidulin. The ARDS models were established by injecting rats with oleic acid. Hispidulin (100 mg/kg) was injected i.p. an hour before ARDS. Myeloperoxidase (MPO), Interleukin-8 (IL-8), Mitogen-activated protein kinases (MAPK), Lipid Peroxidation (LPO), Superoxide Dismutase (SOD), Glutathione (GSH), and Angiotensin-converting enzyme (ACE) were determined by ELISA. Tumor necrosis factor-alpha (TNF-α) expression was described by RT-qPCR. Caspase-3 immunostaining was performed to evaluate apoptosis. Compared with the model group, a significant decrease was observed in the MPO, IL-8, MAPK, ACE, LPO levels, and TNF-α expression in the ARDS+ Hispidulin group. Moreover, reduced caspase-3 immunoreactivity and activity of ACE were detected in the Hispidulin+ARDS group. The protective effect of Hispidulin treatment may act through inhibition of the ACE activity and then regulation of inflammatory cytokine level and alteration of apoptosis.