Browsing by Author "Kesbiç O."

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A comparative evaluation of hematological and biochemical parameters between the italian mullet mugil cephalus (Linnaeus 1758) and the turkish mullet chelon auratus (risso 1810)
(2020-01-01) Fazio F.; Saoca C.; Acar Ü.; Tezel R.; Çelik M.; Yilmaz S.; Kesbiç O.; Yalgin F.; Yiğit M.
This study aimed to carry out a comparative evaluation of the hematological profile (erythrocyte count, hemoglobin concentration, hematocrit, and Wintrobe index parameters such as mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration), in addition to certain biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, serum total protein, albumin, glucose, total cholesterol, and triglycerides), in the Italian mullet Mugil cephalus (Linnaeus 1758) and the Turkish mullet Chelon auratus (Risso 1810). Accordingly, two groups of fish were used in this study: 30 flathead grey mullets, M. cephalus, caught in Lake Faro, Italy, and 30 golden grey mullets, C. auratus, caught in the estuarine channel system of Köyceğiz–Dalyan, Turkey. Statistical evaluations (unpaired t-test) revealed that there were no significant differences in weight or total length values between the two mullet groups. However, the differences between all the evaluated blood parameters (except alanine aminotransferase) between the Italian and the Turkish mullet were significant (P < 0.0001). The present study demonstrated that there were significant hematological and biochemical differences between the Italian (M. cephalus) and the Turkish (C. auratus) mullet. Our findings also contribute to expanding the knowledge on the hematology and biochemistry of two different species of mullet originating from two different habitats. Since the evaluation of blood parameters represents an essential tool in examining the effects of environmental conditions on fish physiology and, consequently, on fish health status, further research in this field is encouraged and would be very useful.
Beneficial effects of Oral Allspice, Pimenta dioica powder supplementation on the hemato-immunological and serum biochemical responses of Oreochromis mossambicus
(2016-09-01) Güllü K.; Acar Ü.; Kesbiç O.; Yılmaz S.; Ağdamar S.; Ergün S.; Türker A.
The present study investigated the effects of dietary allspice powder supplementation on welfare status of Tilapia, Oreochromis mossambicus assessed by hemato-immunological and serum biochemical parameters. Five diets were formulated to contain 0 (control), 5, 10, 15 or 20 g of allspice kg−1 of fish feed. Fish were fed experimental diets for 60 days. Supplementation of allspice powder at 10 g kg−1 positively influenced the serum glucose, plasma lysozyme activity and myeloperoxidase activity. Dietary allspice powder at 15 g kg−1 also positively influenced the serum biochemical parameters (total protein, albumin and globulin) and plasma lysozyme activity. However, 20 g kg−1 allspice powder group had significantly lower values of respiratory burst activity and red blood cell count than other experimental groups (P < 0.05). In conclusion, the results of the present study demonstrated that supplementation of allspice powder at 10 or 15 g kg−1 for 60 days, had beneficial effects on improvement of some immunological and serum biochemical status of O. mossambicus. These findings suggest that dietary supplementation of allspice powder might further improve the resistance to fish pathogens.
Comparative study on haematological and biochemical parameters of two wild sparid fish species
(2019-01-01) Acar Ü.; Saoca C.; Kesbiç O.; Yilmaz S.; Yigit M.; Inanan B.; Fazio F.
Different feeding habits may affect the blood parameters in fish species of the same family, even if they live in the same marine habitat. The aim of this study was to compare some haematological and biochemical parameters of two marine fish species of the same family, the saupe Sarpa salpa (Linnaeus, 1758) and the common two-banded seabream Diplodus vulgaris (Geoffroy Saint-Hilaire, 1817), and to establish the similarities and differences between these species. For this purpose red blood cells (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), glucose (GLU), triglycerides (TRIG), cholesterol (CHOL), serum total protein (TP), albumin (ALB), globulins (GLO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined for 30 fish from each species. Statistical analysis showed significant differences in some haematological and biochemical (RBC, Hb, Hct, TP, ALB, GLO, AST, ALT, ALP and LDH) parameters between the two species. Lower values of RBC, Hb and Hct were found in S. salpa in comparison with D. vulgaris. These differences could be attributed to the feeding behavior, life style and habitat adaptation of the different fish species of the same family and contribute to knowledge of reference values of blood parameters in Sarpa salpa and Diplodus vulgaris. No significant differences were found in MCV, MCH, MCHC and GLU, TRIG, CHOL.
Effects of dietary allspice, Pimenta dioica powder on physiological responses of Oreochromis mossambicus under low pH stress
(2015-12-01) Yılmaz S.; Acar Ü.; Kesbiç O.; Gültepe N.; Ergün S.
This study investigated the effects of the supplementation with allspice (0, 5, 10, 15, or 20 g kg−1) on the haemato-immunological and biochemical variables in tilapia, Oreochromis mossambicus under acidic stress condition. In a 60-day feeding trial, 15 aquariums (80-L) were stocked with 18 fish (20.05 ± 0.10 g) each. Then, acidic stress was achieved by exposing the sampled fish to acidic water (pH 5.5) for 3 days. Allspice supplementation influenced the haematological indices, serum glucose, protein, globulin and innate immune parameters such as respiratory burst activity, lysozyme, and myeloperoxidase activities. In general, at acidic pH decreased circulating red blood cell numbers (RBC), increased mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and the innate immune parameters were observed. On the other hand, the inclusion of allspice prevented an increase in blood glucose MCV and MCH, decreases in albumin, RBC, lysozyme activity and respiratory burst avtivity. In conclusion, this study demonstrated that allspice supplementation at 10 g kg−1 for 60 days, has adequate beneficial effects on improvement of haemato-immunological and biochemical status of O. mossambicus after stressful management.
Effects of dietary Bergamot (Citrus bergamia) peel oil on growth, haematology and immune response of European sea bass (Dicentrarchus labrax) juveniles
(2019-11-01) Acar Ü.; Kesbiç O.; İnanan B.; Yılmaz S.
The aim of the present study was to evaluate the effects of essential oil extracted from bergamot (Citrus bergamia) peel on growth performance and health status of European sea bass juveniles (5.10 + 0.05 g). Test diets supplemented with 0.0%, 0.5%, 1.0% and 2.0% bergamot peel oil (BPO0, BPO0.5, BPO1 and BPO2) were fed to experimental fish for 60 days. At the end of the experiment, the best growth performance was obtained in the BPO0.5 and BPO1 groups (p <.05). No significances were found for haematological parameters including red blood cell count, haematocrit and haemoglobin concentration at the end of the feeding experiment. There were significant differences between the total protein, cholesterol, alkaline phosphatase and glutamic oxaloacetic transaminase (p <.05) between the BPO0.5 and BPO1 supplemented groups and the control group with no BPO addition. The addition of BPO showed significant differences in immune parameters such as lysozyme activity, total myeloperoxidase content, superoxide dismutase and glutathione peroxidase activity between BPO0 and BPO supplemented groups (p <.05). It might be concluded that dietary addition of 0.5%-1% BPO could increase the growth performance and feed utilization with improved health status in sea bass juveniles.
Effects of dietary fish oil replacement by unrefined peanut oil on the growth, serum biochemical and hematological parameters of Mozambique tilapia juveniles (Oreochromis mossambicus)
(2014-01-01) Demir O.; Türker A.; Acar Ü.; Kesbiç O.
This research aimed to evaluate the effects of partial or total replacement of fish oil (FO) with unrefined peanut oil (PO) on growth and some physiological parameters of Mozambique tilapia juveniles for 60 days. Three triplicate groups (initial weight 6.36±0.19 g) were fed with isoenergetic and isonitrogenous diets in which FO was replaced with PO in graded increments of 50% (PO0, PO50, PO100). The results showed that weight gain and feed efficiency of fish fed with PO50 diet were statistically higher than in the other two groups (P<0.05). No significant effects were observed on whole body proximate composition of fish fed with the experimental diets (P>0.05). The partial or total replacement of FO with PO did not affect on the hematological and immunological parameters of fish. Serum glucose, total cholesterol and triglyceride were lower in fish fed with PO50 diet (P<0.05). The present study revealed that PO can be used as an alternate source of dietary lipid up to 50% in tilapia feeds without adverse effects on growth performance, hemato-immunological and serum biochemical parameters
Effects of juniper berry oil on growth performance and blood parameters in common carp (Cyprinus carpio)
(2019-01-01) Kesbiç O.
In this study the effects of commercial juniper berry oil (JBO) as a feed additive in diets for common carp (Cyprinus carpio) was investigated in terms of growth performance, feed utilization and blood parameters. The experimental diets were prepared to contain JBO at rate of 0 (JBO-0), 5 (JBO-5) and 10 (JBO-10) ml/kg. Common carps (3.07 ± 0.15 g) were fed with test diets for a period of 60 days in 40-L aquariums of triplicate groups. The feeding experiment revealed that JBO groups showed significantly better growth performance and feed utilization compared with the control group (JBO-0) (p < 0.05). No significant effect was observed on hemoglobin amount, haematocrit ratio and consequential erythrocyte indices (p > 0.05). In the JBO groups, serum cholesterol, triglyceride, glutamic–pyruvic transaminase and alkaline phosphatase levels decreased significantly (p < 0.05). In contrast, the total protein level showed a significant increase, while glucose, albumin and lactate dehydrogenase levels did not present any significant variation (p > 0.05). Therefore, dietary addition of 5 ml/kg JBO promoted fish growth and positively influenced the blood parameters in common carps.
Effects of juniper berry oil on growth performance and blood parameters in common carp (Cyprinus carpio)
(2019-01-01) Kesbiç O.; Kesbic, OS
In this study the effects of commercial juniper berry oil (JBO) as a feed additive in diets for common carp (Cyprinus carpio) was investigated in terms of growth performance, feed utilization and blood parameters. The experimental diets were prepared to contain JBO at rate of 0 (JBO-0), 5 (JBO-5) and 10 (JBO-10) ml/kg. Common carps (3.07 ± 0.15 g) were fed with test diets for a period of 60 days in 40-L aquariums of triplicate groups. The feeding experiment revealed that JBO groups showed significantly better growth performance and feed utilization compared with the control group (JBO-0) (p < 0.05). No significant effect was observed on hemoglobin amount, haematocrit ratio and consequential erythrocyte indices (p > 0.05). In the JBO groups, serum cholesterol, triglyceride, glutamic–pyruvic transaminase and alkaline phosphatase levels decreased significantly (p < 0.05). In contrast, the total protein level showed a significant increase, while glucose, albumin and lactate dehydrogenase levels did not present any significant variation (p > 0.05). Therefore, dietary addition of 5 ml/kg JBO promoted fish growth and positively influenced the blood parameters in common carps.
Effects of prebiotic mannanoligosaccharides (MOS) on histology and biochemical blood parameters of gilthead seabream, Sparus aurata
(2014-01-01) Gültepe N.; Kesbiç O.; Acar Ü.; Gökkuş K.; İrşad Gültepe M.; Sönmez A.; Bilen S.; Aydın S.
A 90-day growth trial was conducted to determine the effects of prebiotic mannanoligosaccharides (MOS) on the histology and biochemical blood parameters of the gilthead seabream (Sparus aurata). Two experimental diets were formulated to contain MOS (Bio-Mos®) levels of 0 and 2 g/kg. The fish averaging 172.11±13.19 g were reared in six octagonal net cages (763 m3). Fish were fed twice daily to apparent satiation. Individual body weight, weight gain, survival rate and feed conversion rate FCR were measured during the experiment. The treatments produced significant differences in body weight. and weight. gain (p<0.050) but no significant effect on the survival rates and FCR (P>0.05). At the end of the experimental period, biochemical blood parameters were analyzed. Blood urea level was significantly affected in the MOS group (P<0.05). No remarkable alterations in histological examinations were found. This study highlighted the positive effects of prebiotic MOS on protein efficiency and weight gain of gilthead seabream.
Effects of replacing fish meal with peanut meal (Arachis hypogaea) on growth, feed utilization and body composition of mozambique tilapia fries (Oreochromis mossambicus)
(2014-01-01) Yildirim O.; Acar U.; Türker A.; Sunar M.; Kesbiç O.
The study was to determine the effects of diets replacing fish meal (FM) with peanut meal (PNM) on feed utilization, growth performance and body composition of Mozambique tilapia (Oreochromis mossambicus, initial body weight 0.83±0.17 g). Five isonitrogenous (37% CP) and isolipidic (9% CL) experimental diets with 0% (PNM- 0), 10% (PNM-10), 20% (PNM-20), 30% (PNM-30) and 40% (PNM-40) of fish meal replaced with PNM. At the end of a 45 day feeding trial, PNM-10 and PNM-20 diets showed the best growth performance and feed evaluation. Higher dietary FM replacement negatively affected growth performance and feed evaluation. Our results at the end of the experiment showed that the replacement of FM by PNM in Mozambique tilapia diets does not affect body composition. In conclusion, these results indicate that the replacement of 20% dietary FM with PNM had no adverse effect on the growth performance, body composition parameters and general health of Mozambique tilapia fries (mean weight 0.8-3 g). Copyright 2014 Zoological Society of Pakistan.
Effects of Tank Color on Growth Performance and Nitrogen Excretion of European Seabass (Dicentrarchus labrax) Juveniles
(2016-03-01) Kesbiç O.; Yiğit M.; Acar Ü.
The present study investigated feed efficiency and growth performance of juvenile Seabass (Dicentrarchus labrax) cultured in tanks with different colors. Triplicate groups of experimental fish were distributed into 12 tanks, which showed four different tank colors, i.e. red, blue, green, and yellow. All the fish in the trial received the same diet for a period of 60 days. Forty fish with an average weight of 44 g were stocked in the tank. All experimental tanks were supplied with seawater of 22 ppt salinity with temperature ranging between 17–20 °C during the course of the experiment. Tank water volume in the experimental facility of the recirculating aquaculture system (RAS) was set to 40 L. The RAS system consisted of bio-filtration, UV filtration and gravel-mechanic filtration units. Results in the present study demonstrated that growth performance and feed utilization were affected by the tank colors tested in the trial. It is concluded that the selection of appropriate tank color might affect economic benefits in aquaculture facilities.
Evaluation of dietary protein and lipid requirements of two-banded seabream (Diplodus vulgaris) cultured in a recirculating aquaculture system
(2014-01-01) Bulut M.; Yiğit M.; Ergün S.; Kesbiç O.; Acar U.; Gültepe N.; Karga M.; Yılmaz S.; Güroy D.
The objective of this study is to investigate the effects of dietary protein and lipid levels on the growth performance and bioeconomic benefits of two-banded seabream (Diplodus vulgaris) juveniles, a candidate species for aquaculture sector. Eight experimental diets were formulated with four protein (50, 45, 40 and 35 %) levels for each of the two lipid levels (15 and 10 %). Triplicate groups of juvenile fish with an average initial body weight of ~3.64 g were reared in a recirculating aquaculture system and hand fed twice a day until satiation for a period of 60 days. In the experiment, no difference in survival rate was found between the different groups. Relative growth rate (RGR), specific growth rate (SGR), feed conversion ratio (FCR) and daily feed intake were not significantly affected by increasing protein and/or lipid treatments in this present study. However, the RGR, SGR and FCR values showed slightly better efficiency in the experimental group (35/15) fed with lower protein content (35 %) and higher lipid level (15 %) compared with those fed other diets. According to bioeconomic analyses results, the diet with the 35 % protein and 15 % lipid generated the best profit. The results suggest that two-banded seabream can be accepted as a promising alternative species for the aquaculture industry and optimum growth of two-banded seabream fingerlings can be obtained when they are fed a diet containing 35 % crude protein and 15 % crude lipid. © 2013 Springer Science+Business Media Dordrecht.
Pre-challenge and post-challenge haemato-immunological changes in Oreochromis niloticus (Linnaeus, 1758) fed argan oil against Lactococcus garvieae
(2017-08-01) Baba E.; Acar Ü.; Yılmaz S.; Öntaş C.; Kesbiç O.
The present study investigated the effects of argan oil, obtained from Argania spinosa, on pre- and post-challenge immuno-haematological and biochemical responses of Nile tilapia, Oreochromis niloticus. For this purpose, the fish were fed diets containing 0, 0.5%, 1% or 2% argan oil for 45 days. Following 45 days of feeding, fish were challenged with Lactococcus garvieae and mortality was recorded for 15 days. During the pre-challenge period, significantly higher respiratory burst activity, total white blood cell (WBC), serum lysozyme activity and myeloperoxidase activity were determined in the argan oil-fed groups. The serum glucose and cholesterol levels decreased whilst total protein and albumin did not change in the groups fed with argan oil-supplemented diets. After challenge with Lactococcus garvieae, the percentage survival (%) was found to be the highest in the 1% and 2% argan oil-supplemented feeding groups. Also, there was a significant increase in weight gain, specific growth rate and feed conversion ratio in those fish fed argan oil. The results of this study indicated that after the supplementation of fish diets with argan oil, especially at 1% and 2% concentrations, the immunological, haematological and biochemical values remained similar in both the pre- and post-challenge periods and the immune response against L. garvieae in Nile tilapia was modulated.
Structural and chemical changes of grape seed extract after thermal processing and its use in rainbow trout (Oncorhynchus mykiss) diets as an organic feed supplement
(2019-03-30) Kesbiç O.; Yigit M.
The present study aimed to evaluate physicochemical properties and structural variations of grape seed extract exposed to thermal process during feed production, and the use of grape seed extract in rainbow trout diets as a sustainable organic fish feed supplement. Structural changes of grape seed extract granules were observed under a scanning electron microscope, and analyzed using HPLC/UV and GC/MS. Following thermal process, an increase in the granular size of grape seed was observed and cracks were formed on the granules. In the flavor analysis performed using a GC/MS device, totally nine volatile compounds were identified and three of them were palmitic, linoleic and oleic fatty acids. Thermal processing treatment decreased (+)-catechin amount in grape seed extract from 0.667 to 0.557 mg/L, whereas (−)-epicatechin increased from 0.819 to 1.156 mg/L. As a second step of the study, grape seed extract, a waste product of the wine industry, was supplemented in rainbow trout (Oncorhynchus mykiss) diets at concentrations of 0, 0.5, 1, and 2‰ and given to experimental fish for a period of 90 days. Feed and protein utilization coefficients as well as growth performance of the experimental fish fed diets containing grape seed extracts differed significantly (P <.05) compared to the control group fed diets without grape seed extract supplementation. Dietary incorporation of grape seeds had no significant effect on the fat, moisture or ash contents in fish fillets (P >.05), however protein ratio significantly increased in the 0.5‰ grape seed extract group compared to the control (P <.05). Based on these findings and the correlation between dietary grape seed levels and percent growth rates, grape seed extract incorporation of 1.05‰ seems beneficial for the best growth performance and meat quality in rainbow trout. Further, increased levels of (−)-epicatechin (a polymeric flavan-3-ol), known to participate in many physiological regulatory mechanisms likely have provided higher antioxidant activity after thermal processing. Thus, thermal treatment of grape seed extract during feed production might have increased the diet quality with promising results as a potential nutritional supplement in diets for rainbow trout.
Temporal Variations in Hematological, Immunological and Serum Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) Acclimated to High-Saline Water in the Northern Aegean Sea
(2023-01-01) Buyukates Y.; Kesbiç O.; Yigit M.; Yilmaz S.; Ergün S.; Bulut M.; Ozalp B.
The present study evaluated the health status of rainbow trout (30.24±5.34 g) acclimated to high-saline water (28‰). Among adaptation procedures, gradually-acclimated (for 48 h) fish showed best performance, which were then introduced to the offshore cage-farm for further monitoring of fish health in marine environment over a long-term period of 100 days, until harvest weight of 319.9±48.51 g. Fish health and welfare was evaluated by means of biochemical parameters (viscerasomatic index, hepatosomatic index, mesenteric fat index, and spleen somatic index), hematological parameters (serum glucose, triglyceride, cholesterol, total protein, albumin, globulin), and immune parameters (lysozyme, myeloperoxidase, respiratory burst and potential killing values). Gradual acclimation to high-saline water did not show any adverse effects on health parameters. No significant differences were found in biometric measures (P>0.05). However, a time-dependent increase was recorded in hematology markers (P<0.05) after seawater transfer, and the health status improved over long term from March 7 to June 14, 2018. All serum biochemical markers, except the total protein showed significant alterations (P<0.05) in long term but without detrimental influence by day 100. Therefore, it seems possible to expand trout farms from brackish water sites to higher saline environments up to 28‰ salinity, with no detrimental impacts on fish health, that in turn may significantly contribute to the extension of potential aquaculture sites to wider areas.
The use of Avena sativa extract against Aeromonas hydrophila and its effect on growth performance, hematological and immunological parameters in common carp (Cyprinus carpio)
(2016-01-01) Baba E.; Acar Ü.; Öntaş C.; Kesbiç O.; Yilmaz S.
In this research the effects of oat Avena sativa extract on the non-specific immune system of common carp (Cyprinus carpio) was examined. For this purpose, the fishes (average weight 9.91 ± 1.52 g) were fed with 5 g kg -1, 10g kg-1 and 20 g kg -1 oat extract supplemented diets for 60 days. Following 60 days of feeding, the fishes were injected with Aeromonas hydrophila and mortalities were recorded. Lysozyme and myeloperoxidase activity, improved in all groups that received feed supplemented with oat extract. Serum glucose and cholesterol decreased while total protein and albumin in fish increased with the use of the diet supplement with oat extract. Haemoglobin (Hb), mean cell hemoglobin (MCH) and mean cell haemoglobin concentration (MCHC) increased with diet supplemented with oat extract. Oat extract at the concentration of 10 g kg-1 showed significantly higher relative percentage survival (67%) when compared with the control against A. hydrophila injection. Also the dietary supplementation with oat extract caused a significant increase in growth parameters (final weight (FW), weight gain (WG), specific growth rate (SGR) and feed conversion ratio (FCR), when compared to non-supplemented control basal diet. The results suggest that A. sativa extract can be used as a feed supplement to enhance fish immune response and disease resistance against A. hydrophila.