Browsing by Author "Kesbiç, Fevziye Işıl"

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C carotenoids extracted from Haloterrigena thermotolerans strain K15: antioxidant potential and identification.
(2022-02-01T00:00:00Z) Kesbiç, Fevziye Işıl; Gültepe, Nejdet
Halophilic archaea are one of the microorganism groups that have adapted to living in high saline environments and are important in terms of their potential use in biotechnology industry. One of the most important compounds they have, carotenoid, is used in food, cosmetics, and medical industries. The selected strain was identified as an extremely halophilic and thermophilic archaeon, Haloterrigena thermotolerans K15, based on morphological, biochemical, and physiological evidence as well as 16S rRNA analysis and screened by a scanning electron microscope and an atomic force microscope for the first time. The carotenoid contents of H. thermotolerans K15 isolated from Salt Lake (Tuz Gölü, Turkey) were determined by RP-HPLC-DAD and their isomers were characterized according to UV-Vis spectra by cis peak intensity and spectral fine structure. In addition to all-trans bacterioruberin as a major carotenoid, many isomers of the bacterioruberin such as monoanhydrobacterioruberin and bisanhydrobacterioruberin were also found. The antioxidant activity of carotenoid extract from H. thermotolerans was analyzed by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging method. The carotenoid extract showed antioxidant activity statistically significantly higher than ascorbic acid and butylated hydroxytoluene as reference compounds (p < 0.05). This is the first study about carotenoid characterization and antioxidant activity of H. thermotolerans K15. The obtained results suggest the potential use of H. thermotolerans K15 products as a substitute for synthesized chemical carotenoids and antioxidants.
Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal.
(2024-04-09) Kesbiç, Osman Sabri; Acar, Ümit; Kesbiç, Fevziye Işıl; Yılmaz, Sevdan
The need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, Oncorhynchus mykiss. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, and immune (TNF-α, IL1-ß and Il-8) and growth-related (GH and IGFI) gene expression were investigated. Five experimental diets (45% protein and 20% lipid) differed in replacement level of FM by PP at rates of 0% (control (PP0)), 25% (PP25), 50%(PP50), 75%(PP75) and 100%(PP100). Fish were fed with experimental diets in triplicate twice daily. The best growth performance was obtained in PP0 and PP25 groups. While fat ratios of fish fillets significantly differed (p < 0.05), there was no significant effects on protein ratios (p < 0.05). There was no significant change in the hematological values of fish, except those fed the PP100 diets, which displayed a reduction in eyrthocyte counts, hemoglobin content and hematocrit. As PP supplementation increased fish showed elevated serum glucose, total protein, cholesterol and myeloperoxidase activity and decreased glutamic pyruvic transaminase and alkaline phosphatase activity. Fish fed diets with between 25 and 75% replacement showed a decline in lactic acid bacteria in the gut. Significant increases in expression were observed in the liver of the PP25 fish relative to the 0% control for all immune and growth-related genes except for IL1-ß. These data suggest that up to 25% of FM can be replaced by PP without any adverse effects on rainbow trout.
Tuz Gölü'nden izole edilen arkelerin (halobacteriales: halobacteriaceae) filogenetik tür tanımlaması, karotenoidlerinin antioksidan aktivitelerinin belirlenmesi
(Kastamonu Üniversitesi, 2020) Kesbiç, Fevziye Işıl
Bu çalışmada, Ankara, Konya ve Aksaray illerinin sınırları arasında bulunan Tuz Gölü’nün farklı noktalarından alınan tuz numunelerinden izole edilen halofilik arke suşlarının karotenoidlerinin belirlenmesi ve karotenoidlerinin sahip olduğu antioksidan aktivitelerin belirlenmesi amaçlanmıştır. MAM JCM 168 besiyerinde kültüre edilen halofilik arke suşlarının antibiyotik duyarlılık testleri, biyokimyasal ve filogenetik analizler ile tür teşhisleri gerçekleştirilmiştir. Analiz sonuçlarına göre 7 farklı halofilik arke suşu % 98 ve üzeri benzerlik oranıyla Halorubrum lipolyticum JCM 13559, Halorubrum sodomense JCM 8880, Halorubrum californiense FV-22B20, Haloarcula salaria HST01-2R, Halorubrum chaoviator DSM 19316, Haloterrigena thermotolerans PR5, Haloarcula japonica JCM 7785 olarak tespit edilmiştir. Türlerin sahip olduğu majör karotenoid all-trans bakterioruberin olarak belirlenmiş ve bakterioruberin izomerlerinin yanısıra likopen, β-karoten ve IDR gibi farklı karotenoid tiplerine de rastlanmıştır. Türlerden ekstrakte edilen karotenoidlerin antioksidan aktiviteleri DPPH radikal süpürme metoduyla analiz edilmiş ve türlere ait IC50 değerleri H. lipolyticum JCM 13559, H. sodomense JCM 8880, H. californiense FV-22B20, H. salaria HST01-2R, H. chaoviator DSM 19316, H. thermotolerans PR5, H. japonica JCM 7785 için sırasıyla; 2,601 µg/mL; 0,904 µg/mL; 1,007 µg/mL; 1,927 µg/mL; 1,402 µg/mL; 1,284 µg/mL ve 1,474 µg/mL olarak tespit edilmiştir.