Browsing by Author "Kayaalti Z."

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Determination of polycyclic aromatic hydrocarbon metabolites in urine of coke oven workers in Turkey and exposure assessment
(2021-01-01) İçli N.; Kayaalti Z.; Söylemezoğlu T.
Coke-oven workers are occupationally exposed to high concentrations of polycyclic aromatic hydrocarbons. In this study, 1-hydroxypyrene, 9-hydroxyphenantrene, and 3-hydroxybenzo[a]pyrene levels were determined in the urine of occupationally exposed coke-oven worker as markers of exposure to polycyclic aromatic hydrocarbons. The validated high-pressure liquid chromatography method with fluorescence detection was performed for metabolite analysis. Average values of 1-hydroxypyrene were 1.26 µmol/mol creatinine and of 9-hydroxyphenantrene 3.68 µmol/mol creatinine in the workers group, and 0.04 and 0.06 µmol/mol creatinine in the control group. While the average value of 3-hydroxybenzo[a]pyrene was 0.007 µmol/mol creatinine in the workers group, it was below the limit of detection in the control group. All of the mean 1-hydroxypyrene values (coke-oven workers overall mean = 1.26 μmol/mol creatinine; high exposure group mean = 1.91 μmol/mol creatinine; low exposure group mean = 0.66 μmol/mol creatinine) obtained in the workers group were higher than the biological exposure index (0.5 μmol/mol creatinine) that warrants occupational exposure proposed by American Conference of Government Industrial Hygienists. Urinary 1-hydroxypyrene mean levels of all coke-oven workers and the high exposure group exceeded the no observed genotoxic effect level of 1 μmol/mol creatinine.
Evaluation of DNA Damage Using 3 Comet Assay Parameters in Workers Occupationally Exposed to Lead
(2015-05-04) Kayaalti Z.; Yavuz I.; Söylemez E.; Bacaksiz A.; Tutkun E.; Sayal A.; Söylemezoʇlu T.
The aim of this study was to investigate the association between DNA damage and blood lead levels in individuals occupationally exposed to lead. To evaluate this association, 61 workers exposed to lead were monitored in terms of DNA damage in blood lymphocytes. The levels of DNA damage were measured according to 3 comet assay parameters, including tail intensity (TI), tail moment (TM), and DNA tail (DNAt). A statistically significant positive correlation was found between the lead levels and TI, TM, and DNAt (p <.01). Smoking had independent effects on DNA damage. A statistically significant difference was observed between smokers and nonsmokers in regards to DNA damage parameters (p <.05). In addition, the lead and DNA damage levels in smokers were found to be significantly higher than the levels observed in nonsmoking workers (p <.05). Our results show that exposure to lead induces genotoxic effects in peripheral lymphocytes, as measured by comet assays.
Lymphocyte DNA damage in Turkish asphalt workers detected by the comet assay
(2014-01-02) Bacaksiz A.; Kayaalti Z.; Soylemez E.; Tutkun E.; Soylemezoglu T.
Asphalt has a highly complex structure and it contains several organic compounds including polycyclic aromatic hydrocarbons and heterocyclic compounds. In this study, comet assay was used to detect the DNA damage in blood lymphocytes of 30 workers exposed to asphalt fumes and 30 nonexposed controls. This is the first report on Turkish asphalt workers investigated DNA damage using the alkaline single cell gel electrophoresis (SCGE). The DNA damage was evaluated by the percentage of DNA in the comet tail (% tail DNA) for each cell. According to our results, workers exposed to asphalt fumes had higher DNA damage than the control group (p < 0.01). The present study showed that asphalt fumes caused a significant increase in DNA damage and the comet assay is a suitable method for determining DNA damage in asphalt workers. © 2013 Taylor & Francis.