Browsing by Author "Karadeniz M."

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ASSESSMENT OF TOTAL PHENOLIC, TOTAL FLAVONOID, METAL CONTENTS AND ANTIOXIDANT ACTIVITIES OF Trametes versicolor AND Laetiporus sulphureus
(2022-01-01) Bulam S.; Karadeniz M.; Bakır T.K.; Ünal S.
In this study, total phenolic and flavonoid contents, biologically important antioxidant activities and precious metal contents of Trametes versicolor (L.) Lloyd and Laetiporus sulphureus (Bull.) Murrill fruiting bodies collected from Kastamonu province with their unique appearances and growing environments were deter-mined. The content of phenolics in methanolic extracts expressed in gallic acid equivalents varied between 77.41 ±1.10 and 272,70 ±2.26 mg/g for T. versicolor and L. sulphureus, respectively. Also, the content of flavonoids in methanolic extracts expressed in quercetin equivalents varied between 13.82 ±0.21 and 44.29 ±0.54 mg QE/mg for T. versicolor and L. sulphureus, respectively. Antioxidant activity of mushroom extracts were expressed as percentage of DPPH radical inhibition and IC50 values. Percentage of inhibition ranged from 15.83% to 61.03% with IC50 value of 5.33 mg/mL for L. sulphureus, while 7.27% to 20.47% with IC50 value of 18.10 mg/mL was obtained for T. versicolor. In addition, mushroom samples were examined for metal content by ICP-OES. While the most abundant precious metals of fruiting bodies were Ca (23.91 ±0.14), P (17.11 ±0.05), Mg (6.77 ±0.02) and Fe (3.84 ±0.02) as mg/kg DW for T. versicolor, they were P (24.52 ±0.09), Mg (4.59 ±0.01), Ca (0.49 ±0.01), and Fe (0.49 ±0.02) as mg/kg DW for L. sulphureus. The results showed that these two mushroom species rich in total phenolic contents can also be a very valuable source of P and Mg. While heavy metals Cu (123.93 ±0.30), Ni (180.99 ±0.64), Pb (54.62 ±0.58), and Cr (35.27 ±0.33) were found as µg/kg DW for T. versicolor, Cu (36.36 ±0.53), Ni (41.51 ±0.86), Pb (3.50 ±1.26), and Cr (8.23 ±0.55) were found as µg/kg DW for L. sulphureus.
Investigation of antioxidant activities of Pleurotus ostreatus stored at different temperatures
(2018-06-01) Bakir T.; Karadeniz M.; Unal S.
In this study, the antioxidant capacity of oyster mushroom (Pleurotus ostreatus) stored in five different temperature environments was investigated by DPPH (1,1 diphenyl-2-picryl hydrazyl) radical scavenging method. The antioxidant capacity results of oyster mushroom extracts were examined by spectroscopic measurements and expressed as percent of inhibition. The IC50 values of mushroom samples were calculated with DPPH method using calibration equations, and change in antioxidant properties was also tried to decipher by SEM images. The IC50 values of Pleurotus ostreatus for four different concentrations were also found in h+20°C > inh+4°C> inh−10°C> inh−20°C> inh−40°C, respectively. As a conclusion, the temperature factor of oyster mushroom in the range of −40°C to +20°C storage conditions is directly proportional to the change of antioxidant properties. The results have shown the importance of logistics and storage conditions in preserving the antioxidant properties of oyster mushroom and similar food samples.
The effect of Melampsorella caryophyllacearum (fir broom rust) on the morphological and anatomical traits of Abies nordmanniana subsp. equi-trojani
(2021-12-01) Özden Keleş S.; Ünal S.; Karadeniz M.
Abies nordmanniana subsp. equi-trojani (Kazdagi fir), an endemic tree species in Turkey, is increasingly threatened by Melampsorella caryophyllacearum, a fungal pathogen affecting growth and development of trees. In this work, the effect of M. caryophyllacearum on tree growth and wood cell anatomical properties were investigated in four study plots at two different altitudes (low vs. high) and aspects (shaded vs. sunny) in the Ilgaz Mountain area, south-western Kastamonu, Turkey. Morphology (stem diameter and height), ring widths and anatomical cell characteristics (tracheid and ray sizes) were compared between asymptomatic and symptomatic trees within each plot. Disease incidence tended to increase with decreasing altitude: trees that were grown at relatively low altitude (approx. 1400 m a.s.l.) showed disease incidence, compared with trees at high altitude (approx. 1700 m a.s.l.). At the lower altitude, symptomatic trees had thinner, shorter stems, whereas stem diameter and height did not differ between asymptomatic and symptomatic trees at the higher altitude. In this study, symptomatic trees showed wider growth rings and greater wood cell sizes than asymptomatic trees in each study plot. The study thus suggests that low altitude may lead to higher pathogen damage in trees, and greater disease incidence may be encouraged by northern aspect.