Browsing by Author "Kara S."

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Genome-wide identification and comparative expression analysis of LEA genes in watermelon and melon genomes
(2017-01-01) Celik Altunoglu Y.; Baloglu M.C.; Baloglu P.; Yer E.N.; Kara S.
Late embryogenesis abundant (LEA) proteins are large and diverse group of polypeptides which were first identified during seed dehydration and then in vegetative plant tissues during different stress responses. Now, gene family members of LEA proteins have been detected in various organisms. However, there is no report for this protein family in watermelon and melon until this study. A total of 73 LEA genes from watermelon (ClLEA) and 61 LEA genes from melon (CmLEA) were identified in this comprehensive study. They were classified into four and three distinct clusters in watermelon and melon, respectively. There was a correlation between gene structure and motif composition among each LEA groups. Segmental duplication played an important role for LEA gene expansion in watermelon. Maximum gene ontology of LEA genes was observed with poplar LEA genes. For evaluation of tissue specific expression patterns of ClLEA and CmLEA genes, publicly available RNA-seq data were analyzed. The expression analysis of selected LEA genes in root and leaf tissues of drought-stressed watermelon and melon were examined using qRT-PCR. Among them, ClLEA-12-17-46 genes were quickly induced after drought application. Therefore, they might be considered as early response genes for water limitation conditions in watermelon. In addition, CmLEA-42-43 genes were found to be up-regulated in both tissues of melon under drought stress. Our results can open up new frontiers about understanding of functions of these important family members under normal developmental stages and stress conditions by bioinformatics and transcriptomic approaches.
Investigation of job satisfaction and burnout of visual arts teachers
(2020-12-01) Kara S.
In this study, it is aimed to examine the job satisfaction and burnout of visual arts teachers in terms of some variables. Based on the causal comparative method; job satisfaction and burnout of visual arts teachers were compared according to variables such as gender, age, marital status, professional seniority and school type. In addition, the relationship between job satisfaction and professional burnout of visual arts teachers was tried to be explained with correlational research design. The sample of the study consists of 308 visual arts teachers working in Ankara, Istanbul, Konya, Mersin, Eskisehir and Sanliurfa. The Maslach Burnout Inventory and Minnesota Job Satisfaction scales were used to collect the research data. According to the findings of the research, job satisfaction and burnout of visual arts teachers show significant differences according to gender, marital status, professional seniority and school type. In addition, there is a significant but negative relationship between visual arts teachers‟ job satisfaction and burnout.