Browsing by Author "Kara M."

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Apoptosis is induced by sub-acute exposure to 3-MCPD and glycidol on Wistar Albino rat brain cells
(2021-10-01) Sevim Ç.; Özkaraca M.; Kara M.; Ulaş N.; Mendil A.S.; Margina D.; Tsatsakis A.
3-chloropropane-1,2-diol (3-MCPD) and its toxic metabolite glycidol were classified by the International Agency for Research on Cancer (IARC) as belonging to group 2B and 2A for humans. This study aimed to determine the sub-acute toxicity of these agents. Rats were exposed to 3-MCPD at 0.87 and 10 mg/kg/bw and glycidol (2,4 and 37,5 mg/kg/bw) for 90 days. miR-21 gene expression levels significantly decreased in all group's cerebellar tissues compared with control. Exposure to 10 mg/kg/bw 3-MCPD showed significant increases in PTEN in brain as compared to control group. The Akt gen expressions were significantly decreased in 3-MCPD and glycidol groups when compared to control group brains. Additionally, Caspase 3 and AIF immunopositivity significantly increased in 3-MCPD high dose and glycidol high dose groups in cerebellum granular layers compared to control. The results of the present study conclude that 3-MCPD and glycidol can induce apoptosis in rat brain tissue.
Can probiotics win the battle against environmental endocrine disruptors?
(2021-01-01) Sevim Ç.; Kara M.
Compounds that have negative effects on the endocrine system are called endocrine-disrupting compounds (EDCs). There are several different types of compounds, with several different usage areas in the environment, which can be classified as EDCs. These chemicals have a wide range of negative health effects in organisms, depending on their target hormone system. EDCs are among the most popular topics of scientific research, as they are widely used and organisms are frequently exposed to these chemicals. There are various exposure routes for EDCs, such as oral, inhalation and dermal exposure. Parabens, phenolic compounds, phthalates, and pesticides are the most common EDCs. Nowadays, intestinal microorganism distribution, probiotics, and food supplements that regulate these microorganisms and their protective effects against various harmful chemicals attract attention. For this reason, many studies have been carried out in this field and certain diet schemes have been created according to the results of these studies. In fact, probiotics are preferred in order to reduce and eliminate the negative effects of harmful chemicals and to ensure that the organism reacts strongly in these conditions. In this review, we will focus on EDCs, their health effects and positive effects of probiotics on EDCs exposure conditions.
Effect of pressing conditions on some surface properties of HDF laminate parquets
(2016-01-01) Kara M.; Yerlikaya Z.; Ateş S.; Olgun Ç.
In this study, the effects of lamination conditions on some commercialhigh density fiberboard(HDF) panels surface quality characteristics, surface strength, impact resistance test(large diameter ball test), curing degree, scratch resistance, abrasion resistance are investigated. For this purpose, HDF panels are produced from mixture wood fibers(50% black pine and 50% beech wood) as 8.3 mm thickness. After panels sanded to 7.3 mm thickness, laminated at different pressures and times. Ithas been found thathigh pressure and short-term press time conditionshave significant effect on surface strength, impact resistance, and abrasion resistance values. The best surface quality measurements are obtained from A1 group, pressure 340 kg/m² and 13 s pressing time(average of surface strength value of this group is 1.83 N/mm2, impact resistance value is 25.25 N and abrasion resistance value is 3650 revolution), but the lowest value is B3 groups, 320 kg/m² pressure and pressing time of 18 s(average of surface strength of this group is 1.58 N/mm2, impact resistance value is 20 N and abrasion resistance value is 3200 revolutions). Besides production factors of HDF panels and laminating material properties, press operating conditionshave been found to be decisive factors in laminate parquet pressing process for high surface quality.
Investigation of irisin level in brucella patients
(2021-01-01) Algul S.; Senol F.F.; Ugras S.; Esmer F.; Kara M.; Ekici P.; Ozcelik O.
This study aimed to determine whether the changes in the metabolism of Brucella patients affect the irisin hormone. Also, it is aimed to investigate the relationships between other laboratory findings used in the diagnosis of brucellosis patients and the irisin hormone. 160 persons (80 brucellosis, 80 controls) were included in this study. Blood was drawn from the people participating in the study following their overnight fast. Irisin level was measured by ELISA method, CRP and sedimentation levels were measured using autoanalysers and commercial kits. It was determined that the irisin level of Brucella patients was statistically significantly higher than the control group (p <0.05). It was determined that there is a negative correlation between irisin level and CRP and sedimentation level in Brucella patients. In Brucella patients, the higher irisin level than the control group showed a negative correlation with CRP and sedimentation levels, which are infection diagnosis criteria; It suggests that the level of irisin can be considered as a supportive diagnostic marker in these patients. Further studies are needed to evaluate irisin hormone as a supportive diagnostic criterion.
Investigation of the effects of probiotics on sub-chronic neonicotinoid toxicity in rats
(2021-10-01) Sevim C.; Akpinar E.; Tsatsakis A.; Yildirim S.; Tzatzarakis M.; Vardavas A.I.; Vardavas C.I.; Kara M.; Gul Z.
Probiotics have been shown to have positive effects when it comes to combating various health issues when consumed, preventing even the absorption of environmental toxins. One of the main environmental toxins encountered today is pesticide residues. Neonicotinoids, widely applied today in countries that have approved of them, are a known class of insecticides with an excellent and effective potency. Neonicotinoids have been shown to cause various toxic effects, either acutely or chronically, on human health and on beneficial insects when exposed. To clarify the assumption that probiotics could counteract these toxic effects, especially on vital organs, the probiotic yeast “Saccharomyces boulardii” (S. boulardii) was tested against the neonicotinoids, acetamiprid (ACE) and imidacloprid (IMI), as it has outstanding physiological and metabolic properties. The results obtained from the studies indicated that although ACE and IMI induced liver, kidney, brain and bowel damage, there was a considerable level of protection by the dietary supplementation of S. boulardii, as it reduced the absorption of these insecticides.
Reproductive Effects of S. boulardii on Sub-Chronic Acetamiprid and Imidacloprid Toxicity in Male Rats
(2023-02-01) Sevim Ç.; Akpınar E.; Aksu E.H.; Ömür A.D.; Yıldırım S.; Kara M.; Bolat İ.; Tsatsakis A.; Mesnage R.; Golokhvast K.S.; Uzunçakmak S.K.; Ersoylu R.N.
The potential health-promoting effects of probiotics against intoxication by pesticides is a topic of increasing commercial interest with limited scientific evidence. In this study, we aimed to investigate the positive effects of probiotic Saccharomyces boulardii on the male reproductive system under low dose neonicotinoid pesticide exposure conditions. We observed that acetamiprid and imidacloprid caused a degeneration and necrosis of the spermatocytes in the tubular wall, a severe edema of the intertubular region and a hyperemia. This was concomittant to increased levels of 8-hydroxy-2′-deoxyguanosine reflecting oxidative stress, and an increase in caspase 3 expression, reflecting apoptosis. According to our results, Saccharomyces boulardii supplementation mitigates these toxic effects. Further in vivo and clinical studies are needed to clarify the molecular mechanisms of protection. Altogether, our study reinforces the burden of evidence from emerging studies linking the composition of the gut microbiome to the function of the reproductive system.
The sesquiterpenoid valerenic acid protects neuronal cells from the detrimental effects of the fungicide benomyl on apoptosis and DNA oxidation
(2022-05-01) Kara M.; Öztaş E.; Boran T.; Sevim Ç.; Keskin S.E.; Veskoukis A.S.; Kuzmin S.V.; Tsatsakis A.M.
Background: Valerenic acid (VA), a sesquiterpenoid of the plant Valeriana officinalis, has attracted attention of the research community due to its potential positive role against neurodegenerative diseases induced by chemicals. However, the relevant evidence in the literature is scarce. Therefore, this study aimed to examine the putative protective role of VA on the toxic effects of the fungicide benomyl on SH-SY5Y neural cells. Methods: Cell viability was determined via the MTT and NRU assays, DNA damage was assessed via comet assay and apoptosis was evaluated through the expression of relevant genes. Results: According to the results, exposure of the cells to benomyl enhanced viability inhibition and promoted DNA damage and apoptosis since the expression levels of the genes coding for MAPK8, NF-kB, Bax, Caspase-9 and Caspase-3 were increased. Treatment of the cells with VA ameliorated these effects in a concentration dependent manner. Conclusion: It is concluded that the molecular mechanism through which benomyl exerts its toxic action appears to depend on DNA oxidation and apoptosis induction. Furthermore, VA, a plant-derived compound is a protective antioxidant against pesticide-induced toxicity. Therefore, herbs, extracts and compounds of plant origin could be used as nutritional supplements that back up the beneficial role of medicine in neurodegenerative diseases.
“What about users?”: Development and validation of the mobile information security awareness scale (MISAS)
(2021-03-15) Erdoğdu F.; Gökoğlu S.; Kara M.
Purpose: The current study aimed to develop and validate Mobile Information Security Awareness Scale (MISAS) based on the prototype model for measuring information security awareness and the relevant literature. Design/methodology/approach: The scale was developed and validated with the participation of 562 students from four universities. The construct validity of the scale was tested through exploratory factor analysis and confirmatory factor analysis. Findings: The reliability of the scale was tested through corrected item-total correlations and Cronbach alpha. The MISAS includes six factors and 17 items. The identified factors were labeled as backup, instant messaging and navigation, password protection, update, access permission and using others' devices. Research limitations/implications: The scale included only the human aspects of mobile information security. The technical aspects are not within the scope of this study. For this reason, future studies might develop and validate a different scale focusing on the technical aspects of mobile information security. Originality/value: The developed scale contributes to the literature on the human aspects of mobile information security.