Browsing by Author "Kaplan, A.A."

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Preliminary study on the impact of 900 MHz radiation on human sperm: An in vitro molecular approach
(Elsevier Inc., 2024) Keskin, I.; Karabulut, S.; Kaplan, A.A.; Alagöz, M.; Akdeniz, M.; Tüfekci, K.K.; Davis, D.L.; Kaplan, S.
The use of technologies that produce and emit electromagnetic fields (EMF) is growing exponentially worldwide. The biological effects of EMF-emitting equipment, such as mobile phones and other wireless devices, have been studied in the last decade using in vitro and in vivo methods. Infertility is a growing health problem, and nearly half of cases are because of male-factor. This study investigated the direct in vitro effects of 900 MHz radiation exposure on sperm parameters, genetic status, apoptotic markers, and the PI3K/AKT signaling pathway in healthy normozoospermic men. Semen samples were divided into four groups, two control (30 min and 1 h) and two EMF exposure (30 min and 1 h). Sperm parameters (motility, progressive motility, acrosomal index, morphology), genetic status (DNA fragmentation and chromatin integrity), apoptotic markers (cytokine-c and caspase-3 expression) and the PI3K/AKT signaling pathway (phosphoinoitide 3-kinase-PI3K- and phosphorylated AKT- p-AKT-) were analysed. Sperm motility were significantly reduced in 30 min EMF exposure while a significant increase in the expression of p-AKT were observed in 1 h EMF exposure group. An increased vacuolisation, acrosomal defect, extension of subacrosomal space, uncondensed chromatin structure, apoptotic signs and disrupted axoneme were observed in both EMF groups which were not observed in the control group. Other sperm parameters (morphology and acrosomal index), genetic status, apoptotic markers and the PI3K expression rates had no significant change.
Preliminary study on the impact of 900 MHz radiation on human sperm: An in vitro molecular approach
(2024.01.01) Keskin, I.; Karabulut, S.; Kaplan, A.A.; Alagöz, M.; Akdeniz, M.; Tüfekci, K.K.; Davis, D.L.; Kaplan, S.
The use of technologies that produce and emit electromagnetic fields (EMF) is growing exponentially worldwide. The biological effects of EMF-emitting equipment, such as mobile phones and other wireless devices, have been studied in the last decade using in vitro and in vivo methods. Infertility is a growing health problem, and nearly half of cases are because of male-factor. This study investigated the direct in vitro effects of 900 MHz radiation exposure on sperm parameters, genetic status, apoptotic markers, and the PI3K/AKT signaling pathway in healthy normozoospermic men. Semen samples were divided into four groups, two control (30 min and 1 h) and two EMF exposure (30 min and 1 h). Sperm parameters (motility, progressive motility, acrosomal index, morphology), genetic status (DNA fragmentation and chromatin integrity), apoptotic markers (cytokine-c and caspase-3 expression) and the PI3K/AKT signaling pathway (phosphoinoitide 3-kinase-PI3K- and phosphorylated AKT- p-AKT-) were analysed. Sperm motility were significantly reduced in 30 min EMF exposure while a significant increase in the expression of p-AKT were observed in 1 h EMF exposure group. An increased vacuolisation, acrosomal defect, extension of subacrosomal space, uncondensed chromatin structure, apoptotic signs and disrupted axoneme were observed in both EMF groups which were not observed in the control group. Other sperm parameters (morphology and acrosomal index), genetic status, apoptotic markers and the PI3K expression rates had no significant change.
Preliminary Study on the Impact of 900MHz Radiation on Human Sperm: An In Vitro Molecular Approach
(2024) Keskin, I.; Karabulut, S.; Kaplan, A.A.; Alagöz, M.; Akdeniz, M.; Tüfekci, K.K.; Davis, D.L.; Kaplan, S.
The use of technologies that produce and emit electromagnetic fields (EMF) is growing exponentially worldwide. The biological effects of EMF-emitting equipment, such as mobile phones and other wireless devices, have been studied in the last decade using in vitro and in vivo methods. Infertility is a growing health problem, and nearly half of cases are because of male-factor. This study investigated the direct in vitro effects of 900MHz radiation exposure on sperm parameters, genetic status, apoptotic markers, and the PI3K/AKT signaling pathway in healthy normozoospermic men. Semen samples were divided into four groups, two control (30min and 1h) and two EMF exposure (30min and 1h). Sperm parameters (motility, progressive motility, acrosomal index, morphology), genetic status (DNA fragmentation and chromatin integrity), apoptotic markers (cytokine-c and caspase-3 expression) and the PI3K/AKT signaling pathway (phosphoinoitide 3-kinase-PI3K- and phosphorylated AKT- p-AKT-) were analysed. Sperm motility were significantly reduced in 30min EMF exposure while a significant increase in the expression of p-AKT were observed in 1h EMF exposure group. An increased vacuolisation, acrosomal defect, extension of subacrosomal space, uncondensed chromatin structure, apoptotic signs and disrupted axoneme were observed in both EMF groups which were not observed in the control group. Other sperm parameters (morphology and acrosomal index), genetic status, apoptotic markers and the PI3K expression rates had no significant change.
The potential protective effects of melatonin and omega-3 on the male rat optic nerve exposed to 900?MHz electromagnetic radiation during the prenatal period
(2023.01.01) Tuefekci, K.K.; Kaplan, A.A.; Kaya, A.; Alrafiah, A.; Altun, G.; Aktas, A.; Kaplan, S.
Background: Due to children and adolescents' widespread use of electronic devices, researchers have focused on pre-and early postnatal electromagnetic field (EMF) exposure. However, little is known about the effects of EMF exposure on the optic nerve. The aim of study was to investigate the changes occurring in the optic nerve and the protective effects of melatonin (mel) and omega 3 (?-3) in rats.Methods: Thirty-five pregnant rats were divided into seven groups, Cont, Sham, EMF, EMF + melatonin (EMF + Mel), EMF + ?3, Mel, and ?3. The EMF groups were exposed to 900 megahertz (MHz) EMF daily for two hours during pregnancy. After the experiment, the right optic nerve of each offspring rat was removed and fixed in glutaraldehyde. Thin and semi-thin sections were taken for electron microscopic and stereological analyses. Myelinated axon numbers, myelin sheath thicknesses, and axonal areas were estimated using stereological methods.Results: The groups had no significant differences regarding mean numbers of axons, mean axonal areas, or mean myelin sheath thicknesses (p > 0.05). Histological observations revealed impaired lamellae in the myelin sheath of most axons, and vacuolization was frequently observed between the myelin sheath and axon in the EMF-exposed group. The Mel and ?-3-treated EMF groups exhibited well-preserved myelinated nerve fibers and intact astrocytes and oligodendrocytes.Conclusions: At the ultrastructural level, Mel and ?3 exhibits a neuroprotective effect on the optic nerve exposed to prenatal EMF. The protective effects of these antioxidants on oligodendrocytes, which play an essential role in myelin formation in the central nervous system, now require detailed investigation.
Uterus stereology
(Nova Science Publishers, Inc., 2024) Kaya, A.; Altun, G.; Tüfekçi, K.K.; Kaplan, A.A.; Kipanyula, M.J.; Kaplan, S.
Infertility is a growing global health problem with important socio-psychological consequences. The main causes of infertility are the pathogenesis of the uterine mucosa and the uterus being unready for implantation as a result of decidualization. Despite the critical importance of the uterus for women's health and fertility, and embryo development, relatively limited information is available concerning the hormonal, cellular, and molecular mechanisms in its pathogenesis. Morphoquantitative assessments are widely used by researchers to explain the underlying causes of infertility. Quantitative evaluations provide more descriptive and precise information when observing structural changes in tissue under different conditions and comparing these between different groups. Design-based stereology provides an accurate and unbiased estimation of stereological parameters, avoiding sources of bias caused by the methodology employed. It is therefore important to use stereological methods when considering a quantitative morphological evaluation of biological objects. This chapter describes the importance of developing and disseminating the use of stereological techniques in uterine research and is intended to explain the modern stereological techniques applied to uterine tissue in methodological terms.