Browsing by Author "Kandil B."

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Expression profile of Toll-like receptor 4 in rat testis and epididymis throughout postnatal development
(2020-04-01) Özbek M.; Hitit M.; Ergün E.; Ergün L.; Beyaz F.; Erhan F.; Yıldırım N.; Kandil B.; Özgenç Ö.; Memili E.
Toll-like receptors (TLRs) belonging to pattern recognition receptors are involved in maintaining testicular and epididymal immune homeostasis. The purpose of the current study was to investigate TLR4 expression in rat testis and epididymis throughout postnatal development. Weak staining was detected in peritubular myoid cells and immature Sertoli cells while no staining was observed in gonocytes during prepubertal period. However, TLR4 expression began to appear in spermatocytes in pubertal period and gradually increased in spermatids. An intense staining was observed in steps 5–19 spermatids in post pubertal and mature periods. Similarly, TLR4 expression in the testes steadily increased from pubertal period to mature period. Puberty also caused a significant increase in TLR4 expression in epididymis. TLR4 expression in cauda epididymis was lower as compared to those of other epididymal segments. The majority of epididymal epithelial cells exhibited apical TLR4 expression, whereas basal cells showed intense intracytoplasmic immunoreaction. We detected an intense staining in epididymal smooth muscle cells. The expression levels of TLR4 showed dynamic changes in both spermatogenic cells, and entire testicular and epididymal tissues during postnatal development. These results suggest that TLR4 expression contributes not only to inflammation but also to the development of spermatogenic cells.
Morphological structure of rat tongue using light and scanning electron microscopy
(2023-01-01) Demirci B.; Kandil B.; Yüksel S.; Gültiken M.E.
The rat is one of the most commonly used animals in biological research and experimental investigations in medicine. The ultrafine structural components of the epithelium differ depending on the anatomy of the animal and the papilla type. Animal adaptation to food types and environmental circumstances may also be linked to morphological diversity. In the current study, seven male Wistar rat tongues were investigated. For scanning electron microscope (SEM), two rat tongues were immersed in a 10% formalin solution and the other two rat tongues were immersed in a 2.5% glutaraldehyde solution. The tongues of three rats were fixed for regular histological evaluation using triple staining. The three primary components of the Wistar rat tongue are the apex, body and root. The apex had a rounded and bifurcated shape. Filiform papillae and gustatory papillae were easily identified on the dorsal side of the tongue. There were three forms of gustatory papillae; fungiform papillae, vallate papillae and foliate papillae. The purpose of this study was to expose the tongue morphology of the Wistar rat species, which is widely used in investigations. Also, we wanted to show that formalin fixation can be utilized for morphological research in SEM. Finally, the Wistar rat tongue was thoroughly investigated and compared to those of other species.