Browsing by Author "Güney K."

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A 2-year aeropalynological survey of allergenic pollen in the atmosphere of Kastamonu, Turkey
(2012-09-01) Çeter T.; Pinar N.M.; Güney K.; Yildiz A.; Aşci B.; Smith M.
Knowledge of airborne pollen concentrations and the weather conditions influencing them is important for air quality forecasters, allergists and allergy sufferers. For this reason, a 7-day recording volumetric spore trap of the Hirst design was used for pollen monitoring between January 2006 and December 2007 in Kastamonu, Turkey. A total of 293,427 pollen grains belonging to 51 taxa were recorded during the study period. In the 2 years of study, the period March-August was identified as the main pollination season for Kastamonu. The highest monthly pollen counts were observed in May in both years. Six taxa made up 86.5% of the total amount of pollen recorded in the atmosphere of Kastamonu. These were as follows: Pinaceae (42.9%), Cupressaceae (20.6%), Poaceae (9.7%), Quercus (5.5%) Betula (5.3%) and Carpinus (2.6%). Four of these are considered to be highly allergenic (Betula, Carpinus, Cupressaceae and Poaceae). There were also a greater percentage of highly allergenic taxa found within the city, including Betula pendula that is not part of the local flora. This shows that through urban planting, the public and municipalities can unconsciously create a high risk for allergy sufferers. Daily average pollen counts from the six most frequently recorded pollen types were entered into Spearman's correlation analysis with meteorological data. Mean daily temperature, relative humidity, daily rainfall and wind speed were found to significantly (p < 0.05) affect atmospheric pollen concentrations, but the relationships between pollen concentrations and meteorological variables can vary and so there is a need for more local studies of this nature. © 2011 Springer Science+Business Media B.V.
Antimicrobial activities and some flavonoids in extracts of some medicinal plants
(2017-07-01) Şener I.; Gür M.; Verep D.; Güney K.; Altuner E.
Origanum majorana, Melissa officinalis, Anthemis cotula and Avena sativa were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of some flavonoids were analyzed by using HPLC. It was determined flavonoids in the extracts of O. majorana, M. officinalis, A. cotula and A. sativa. As a results it was observed that O. majorana was active against Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis, Salmonella typhimurium and Pseudomonas aeruginosa, where M. officinalis was active against Enterococcus faecium and Enterococcus faecalis. On the other hand, both A. cotula and A. sativa were observed to be active against Enterococcus faecium. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels.
Antimicrobial activity and chemical composition of coriander & galangal essential oil
(2017-07-01) Özkinali S.; Şener N.; Gür M.; Güney K.; Olgun Ç.
Essential oils were obtained from Coriandrum sativum and Alpinia officinarum through steam distillation method in this study. Antimicrobial activities of the obtained essential oils were investigated by using minimum inhibitory concentration (MIC) test by against 18 different species microorganisms. It was found that the C. sativum had strong antimicrobial activities against the 14 tested microorganism and A. officinarum oils showed strong antimicrobial activity against the 7 tested microorganisms. Furthermore, the contents of the essential oils were determined with GC-MS analyses. The most abundant compounds of essential oils of A. officinarum and C. sativum were determined as 28.4% eucalyptol and 69.4% linalool. As a result, it can be easily said that the essential oils of C. sativum and A. officinarum can be used as antimicrobial protector in related fields.
Antimicrobial activity of endemic digitalis lamarckii Ivan from Turkey
(2009-03-01) Benli M.; Yiǧit N.; Geven F.; Güney K.; Ümit B.
Antimicrobial activity of the methanolic extracts of leaves and flowers of D. lamarckii Ivan, (Scophulariaceae), an endemic plant species of Turkey, was tested on ten bacterial and four yeast strains. Effective antibacterial activity was observed in four bacterial strains. Minimum inhibitory concentration (MIC) was calculated by use of liquid culture tests and in all the four effective bacterial strains, the MIC was found to be ≥199.5 mg/ml. The minimum bactericidal concentration (MBC) of B. subtilis, S. aureus, and L. monocytogenes was calculated to be ≥199.5 mg/ml, and MBC value for Shigella was calculated as ≥399 mg/ml.
Antimicrobial activity of some endemic plant species from Turkey
(2007-08-06) Benli M.; Güney K.; Bingöl U.; Geven F.; Yiǧit N.
Six plant extracts obtained from different parts such as the leaves, flowers and seeds of four species of the endemic plants in Turkey were tested on a total of 14 microorganisms, 10 of which were bacterial strains and 4 yeast strains. Verbascum eriocarpum (flower) extract was found to be effective against Staphylococcus aureus; Stachys cretica subsp. anatolica (leaf and flower) and Heracleum paphlagonicum (seed) extracts were found to be effective against Bacillus subtilis; and Alcea apterocarpa (seed and sepal) extract was found to be effective against Pseudomonas aeruginosa. No antimicrobial activity was observed in Heracleum paphlagonicum (leaf) and Alcea apterocarpa (leaf) plant extracts. The minimum inhibitory concentration (MIC) values of the plant extracts were calculated to be between ≥ 0.859 mg/ml and ≥ 110.5 mg/ml and the minimum bacteriocidal concentration (MBC) values were calculated to be between ≥ 3.44 mg/ml and ≥ 132 mg/ml. © 2007 Academic Journals.
Antioxidant enzyme activities and immune responses in rainbow trout (Onchorhynchus mykiss) juveniles fed diets supplemented with dandelion (Taraxacum officinalis) and lichen (Usnea barbata) extracts
(2021-08-01) Salem M.O.A.; Salem T.A.; Yürüten Özdemir K.; Sönmez A.Y.; Bilen S.; Güney K.
In the present study, antioxidant activity, immune responses, and growth performance of rainbow trout (Onchorhynchus mykiss) juveniles fed with diets supplemented with dandelion (Taraxacum officinalis) and lichen (Usnea barbata) extracts were assessed. Four different concentrations of aqueous methanolic extract of the plants (0% (control), 0.1%, 0.5%, and 1% (D, dandelion; L, lichen) were added to the diets, and fish were fed for 75 days. On the 15th, 45th, and 75th day of the study, liver antioxidant enzyme activities were determined, and immune responses were determined every 15th day. The results showed that SOD activity increased in the fish group of 0.1% D on the 15th and 45th day compared to control; however, it was lower in all the lichen extract-treated groups than in control at almost all sampling times, except on the 15th day in the 0.1% L group. CAT activity showed an increased value (P < 0.05) in 0.5% L and 1% L treated fish groups on the 15th day, in fish of 1% D and 1% L groups on 45th and on 75th day in 0.1% D group. GPX activity increased on the 15th day of the study in fish of 0.1% D group, on the 45th day in 1% D and 1% L groups and on the 75th day in fish of 0.5% D, 0.1% D, and 0.5% L groups (P < 0.05). G6PDH enhanced in all treatment groups compared to control on the 15th day, except in 0.1% L and 0.5% L groups. An elevated G6PDH activity was also observed on the 75th day of the study in 0.5% D, 1% D, and 0.5% L fish groups. An increase on lipid peroxidation (LP) was observed in all L groups on the 45th day of the study. Lysozyme activity was determined to be the highest in 0.5% and 1% L on the 45th day, in 0.1% L on the 60th day and in the 0.5% L fish group on the 75th day compared to control (P < 0.05). Myeloperoxidase was found to be the highest at the end of the study in 1% L fish group compared to the control (P < 0.05). In conclusion, we suggest the use of dandelion to combat oxidative stress and to lower FCR and the use of lichen to modulate the immune response in rainbow trout. The use of such products will be economical for aquaculture and harmless for the environment.
Biodiversity of gavurdag wildlife development area
(2017-07-01) Güney K.; Küçük Ö.; Aktürk E.; Evcin Ö.
Natural resources are depleting due to detrimental effects of human interventations. The preservation and rehablitation of these areas are critical. It is very important to identify existing floristic and faunistic elements of these sites for management and future studies. The main purpose of these strategies is to protect and develop the target species and other resource values of the protected area. Besides this, it is also aimed to protect the floristic and faunistic value of the conservation area, in other words protecting the biodiversity. It is aimed to investigate and document the floristic and faunistic values in Gavurdag Wildlife Development Area in this study. According to floristic results, 618 plant taxa were found and 97 of them were detected as endemic in the study field (Endemism ratio: 15.7%). Moreover, when the IUCN categories are examined; 7 species are found to be in En category and 10 species are found to be in the Vu category. In addition, 15 mammal and 64 bird species were detected with faunal research in the field.
Chemical composition and antimicrobial activity in cold press oil of fennel, anise, white and black mustard seeds
(2017-07-01) Olgun Ç.; Özkan O.; Güney B.; Pattabanoglu E.; Güney K.; Gür M.
In this study, the cold press oil components and antimicrobial activities of fennel (Foeniculum vulgare) and anise (Pimpinella anisum) and white mustard (Sinapis alba) and black mustard (Brassica nigra) species seeds, which are widely used by the people for alternative medicine, were determined. F. vulgare, P. anisum, S. alba and B. nigra species seeds were obtained from cultivated areas in central Anatolia in Turkey. The oil was extracted by using a screw press (MP-001 Cold Press, Turkey), and the volatile oil components and fatty acid components in these oils were analysed by GCMS and total phenolic content, total flavonoid content and antioxidant activities by DPPH and FRAP (%) method were determined. Antimicrobial activities of obtained oils were investigated by using minimum inhibitory concentration (MIC) test by against 18 different species microorganisms. In the GCMS results, F. vulgare and P. anisum oils were found to be the most abundant components which were anethole (89.74%, 88.95%, respectively). According to these results, the plants oils didn’t show any antimicrobial activities against tested microorganisms. However especially white and black mustard oils showed strong antioxidant activity when compared with artificial antioxidants.
Determination of antimicrobial activity and chemical composition of pimento & ginger essential oil
(2017-07-01) Şener N.; Özkinali S.; Gür M.; Güney K.; Özkan O.; Khalifa M.
The essential oil was obtained from pimento (Pimenta racemosa Mill.) and ginger (Zingiber officinale Rosc.) through hydrodistillation method. The chemical composition of pimento and ginger essential oils were analyzed by GC-MS. It was found that the most abundant components were methyleugenol (52.33%) and zingiberene (16.32%) in essential oils of pimento and ginger, respectively. The antimicrobial activities of essential oils were investigated against 18 microorganisms with minimum inhibitory concentration (MIC) method. While pimento essential oil behaved strong antimicrobial against all bacteria apart from S. epidermidis, ginger essential oil exhibited poor activity against all bacteria. As a result of this study; it can be suggested that pimento essential oil is a preservative in the medicinal application and food industry.
Determination of some flavonoids and antimicrobial behaviour of some plants’ extracts
(2017-07-01) Gür M.; Verep D.; Güney K.; Güder A.; Altuner E.
C. sativa, C. intybus, L. stoechas, V. officinalis and G. glabra plants were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of eight flavonoids were analysed by using HPLC. It was found that C. sativa is active against C. albicans, E. faecalis, S. enteritidis and S. typhimurium with MIC values of 26.02 µg/mL, 13.01 µg/mL, 416.25 µg/mL and 832.50 µg/mL respectively, where C. intybus is active against C. albicans and E. faecalis, with MIC values of 13.01 µg/mL and 6.50 µg/mL, respectively. On the other hand, L. stoechas and V. officinalis were observed to be active against only S. enteritidis with MIC values of 52.03 µg/mL and 26.02 µg/L respectively, where G. glabra was active against only E. faecalis, with a MIC value of 52.03 µg/mL. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels. But the activities against C. albicans and E. faecalis is noteworthy. The flavonoids were determined at different amounts in extracts.
Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss)
(2020-02-01) Bilen S.; Altief T.A.S.; Özdemir K.Y.; Salem M.O.A.; Terzi E.; Güney K.
This study was conducted to determine the effects of dietary supplementation of lemon balm (Melissa officinalis) aqueous methanolic extract on growth performance, blood parameters, digestive and antioxidant enzyme activities, and non-specific immune responses in rainbow trout (Oncorhynchus mykiss). Fish with an average weight of 23.03 ± 0.07 g were fed a diet supplemented with an aqueous methanolic extract of lemon balm at a dose of 0 (control), 0.1 (LB0.1), 0.5 (LB0.5), and 1 g kg−1 (LB1) for 75 days. The final weight, weight gain, and specific growth rate were observed to be significantly increased in LB0.5 and LB1 groups compared with that of the control. No differences were observed in feed conversion ratio values. WBC increased at the 45th day of the study in LB0.1 group. No differences were determined in RBC (P > 0.05). At the end of the study, lipase increased significantly in all experimental groups compared with the control. Pepsin was also elevated in LB0.5 and LB1 groups compared with the control. Increased trypsin was determined in LB1 group (P < 0.05). SOD activity increased at the end of the study in LB1 group (P < 0.05). CAT values had no differences compared with control. GR activity increased in all experimental groups compared with control. GPx improved in LB0.5 and LB1 groups significantly (P < 0.05). Lipid peroxidation was decreased in LB1 group compared with that of control, but this decrease was not significant (P < 0.05). Oxidative radical production and lysozyme activity significantly increased in LB1 group (P < 0.05). The highest MPO was determined in control group (P < 0.05). Current results suggest that lemon balm extract stimulates growth promoting antioxidant and immune responses in rainbow trout.
Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss)
(2021-06-01) Bilen S.; Ispir S.; Kenanoglu O.N.; Taştan Y.; Güney K.; Terzi E.
This study investigated the effects of Greek juniper extract on immune responses of rainbow trout. In this experiment, 4 doses [0 (Control), 1 (J1), 4 (J4) and 8 (J8) mg/kg] of the extract were administered orally using an oral gavage twice a day for 14 days. Immune responses were measured on 7th and 14th days. On 14th day, Yersinia ruckeri was injected intraperitoneally to all fish of all groups. On 14th day, ORP in fish of J1 group increased significantly. Lysozyme activity (LA) was increased in J8 group on 7th day (p <.05). On 14th day, a significant decrease was determined in J1 and J4 treatments in LA. Myeloperoxidase activity was significantly decreased in all groups irrespective of sampling times (p <.05). Interleukin (IL)-1β was significantly elevated in fish of J8 group on 7th day. IL-8 increased in fish of J8 and J4 groups on 7th day of the study. IL-12 gene expression was significantly up-regulated in J8 fish group on 7th day, and in J4 fish group on 14th day. Survival rate was higher in J8 treatment compared to the control and other treatments (p <.05). The results suggest that Juniperus excelsa provides protection against Y. ruckeri in rainbow trout.
Essential oil of oregano and savory; chemical composition and antimicrobial activity
(2017-07-01) Özkan O.; Güney K.; Gür M.; Pattabanoğlu E.; Babat E.; Khalifa M.
The essential oils of oregano (Origanum vulgare ssp. hirtum) and savory (Satureja thymbra) plants were obtained through steam distillation method. The chemical compositions of their essential oils were analyzed via GC-MS technique. Furthermore, their antimicrobial activities were studied by using minimum inhibitory concentration (MIC) method. Their chemical composition was found as carvacrol (63.97%), p-cymene (12.63%) and linalool (3.67%) in oregano essential oil as major compounds and also, as carvacrol (42.7%), o-cymene (17.98%), linalool (9.65%), caryophyllene oxide (5.25%) and γ-terpinene (4.22%) in savory essential oil as major compounds. As a result, it can be said that that oregano and savory essential oil may be useful in the medicinal application and food industry as a preservative against harmful microorganisms.
New host plant for the species Agapanthia lateralis Gangl. (Coleoptera; Cerambycidae)
(2014-01-01) Šálek L.; Güney K.
We have found a new host plant for the long-horn beetle Agapanthia lateralis Gangl. in the north Turkey in the city Kastamonu and in its surroundings. The adults hatched from stalks of herb Onobrychis hypargyrea Boiss. where larvae were found. The diet of Agapanthia lateralis is broader than previously thought and a larger diversity of host plants can probably enable them to occur in more diverse habitats.
New Syntaxa for Alliance Astragalo karamasici-Gypsophilion from Central Anatolia (Polatlı-Haymana)
(2009-01-01) Geven F.; Ketenoǧlu O.; Bingöl U.; Güney K.
In this study, the steppe vegetation between Polatli{dotless}-Haymana had been analyzed. The study area is located in the southwest part of Central Anatolia covered with the gypsaceous and marly-gypsaceous soils. The vegetation which is under the effective control of semi arid very cold type of Mediterranean climate was analyzed by the three dimensional ordination technique based on the Braun-Blanquet method. Two new associations were described and classified syntaxonomically. These associations which are defined for the first time are named according to the rules of "International Code of Phytosociological Nomenclature". Associations and their higher units are as follows: Class: Astragalo-Brometea Quezel 1973 Order: Onobrychido armeni-Thymetalia leucostomi Akman, Ketenoglu, Quezel 1985 Alliance: Astragalo karamasici-Gypsophillion eriocalycis Ketenoglu, Quezel, Akman, Aydogdu 1983 Association: Salvio-Astragaletum microcephali ass. nova Association: Minuartio-Acantholimetum acerosi ass. nova Phytosociological and phytoecological features of these associations had been discussed and compared with the similar assosiations.
Pollen morphology of some Verbascum (Scrophulariaceae) taxa in Turkey
(2018-01-09) Öztürk A.; Güney K.B.; Bani B.; Güney K.; Karavelioğullari F.A.; Pinar N.M.; Çeter T.
Pollen morphology of six Verbascum (Scrophulariaceae) taxa belonging to five different groups in the genus [V. georgicum (group E), V. nudatum var. nudatum (group F), V. cariense (group F), V. bithynicum (group H), V. pycnostachyum (group K), and V. lasianthum (group L)] was studied. Pollen slides were prepared according to the Woodhouse method and were examined with light-microscopy (LM) and Scanning Electron Microscopy (SEM). All studied pollen grains are generally monads, though tetrads (5 %) were observed in Verbascum nudatum var. nudatum. Pollen grains of all studied taxa were determined as isopolar and radially symmetric. In general terms, aperture types are trizonocolporate, but also trisyncolpate (V. lasianthum 10 %, V. pycnostachum 1 %, and V. georgicum 4 %) and trizonocolpate (V. cariense 10 %, V. bithynicum 10%, V. lasianthum 90%, V. pycnostachyum 9%, and V. georgicum 96 %) aperture types were observed. The shapes of the pollen grains were determined as prolate, subprolate, prolate-spheroidal and oblate-spheroidal. The sculpture was reticulate in all cases. The results of the study corroborate that morphological features, such as pollen shape, polar axis length (P), equatorial axis length (L), aperture type, surface ornamentation, muri thickness, and lumina shape, show differences which can be taken into consideration in the systematic discrimination within the groups, and also for discrimination of the groups in the genus.
Prevention of Saprolegniasis in rainbow trout (Oncorhynchus mykiss) eggs using oregano (Origanum onites) and laurel (Laurus nobilis) essential oils
(2022-01-01) Özdemir R.C.; Taştan Y.; Güney K.
The present study investigated the antifungal effects of essential oils of oregano (Origanum onites) and laurel (Laurus nobilis) on Saprolegniasis, a disease that occurs in rainbow trout eggs during the incubation period. Oregano and laurel were ground after drying, and essential oils were obtained by water distillation method using a Clevenger apparatus. The essential oils were added to potato dextrose agar (PDA) at the rates of 1–1000 ppm, and the minimum inhibitory concentration (MIC) was determined as 250 ppm whereas the minimum lethal concentration (MLC) was determined to be 500 ppm for both plants. In the in vivo trials, fertilized eggs were treated with predetermined doses either by bathing during water hardening and incubation period or only during incubation period, and death rates were monitored during embryological development. The best larvae hatching rate was determined in 500 ppm oregano and 500 ppm laurel groups treated during water hardening plus daily as 82.11% and 79.87%, respectively. According to the results, it was determined that oregano and laurel essential oils exhibited better results in all doses compared with the negative control group, and 500 ppm dose had a better effect than the positive control group treated with formalin.
Seasonal variation of hypericin and pseudohypericin contents in Hypericum scabrum L. growing wild in Turkey
(2008-01-01) Ayan A.K.; Çirak C.; Güney K.
The present study was conducted to determine ontogenetic and morphogenetic variations of hypericin and pseudohypericin contents in Hypericum scabrum growing in Turkey. Plants were harvested at vegetative, floral budding, full flowering, fresh fruiting and mature fruiting stages and observed for the presence of dark glands. Subsequently, they were dissected into stem, leaf and reproductive tissues, which were dried separately, and subsequently assayed for hypericin and pseudohypericin contents by HPLC. No hypericins were detected in stem tissues, while leaves and reproductive parts accumulated both compounds at different levels depending on growth stages. In general, higher levels of hypericin and pseudohypericin accumulation were observed in reproductive parts. Content of both hypericin forms decreased with advancing of plant development and reached their highest levels at floral budding stage.
Some Important Plants for Epilepsy Treatment: Antioxidant Activity and Flavonoid Compositions
(2018-12-01) Gür M.; Güder A.; Verep D.; Güney K.; Özkan O.E.; Seki N.; Kandemirli F.
Epilepsy, a disorder of the brain, is a major health problem that the widespread availability and use of herbal medicines raise the potential for adverse effects in the epilepsy population. Although many synthetic antiepileptic drugs are available in operation, their efficiency does not verify with the all population suffering from this disorder because of the major restrictions such as side effects and the drug interactions in its clinical utility. This paper is concerned with antioxidant activity, total phenolic and flavonoid contents and flavonoid composition of the 14 plants for three different solvents (water, ethanol and hexane). For this reason, FRAP, DPPH free radical scavenging, metal-chelate and H2O2 scavenging activities were investigated. In addition, total phenolic and flavonoid contents of extracts were determined via spectroscopic techniques. Lastly, composition of catechin, epicatechin, rutin, naringin, myricetin, luteolin, naringenin and apigenin was found with normal-phase HPLC in the obtained extracts. Extracts of fourteen plants taxa foreseen in having antioxidant and antiepileptic features were obtained with soxhlet extraction using different solvents, i.e., water, ethanol and hexane in this study. The extracts of 14 plant taxa were acquired: French lavender (Lavandula stoechas L.—inflorescence), Valerian (Valeriana officinalis L.—herb), Syrian rue (Peganum harmala L.—seeds), Liquorice (Glycyrrhiza glabra L.—roots), Boxwood (Buxus sempervirens L.—leaves), Chicory (Cichorium intybus L.—herb), Marjoram (Origanum majorana L.—leaves), Lemon balm (Melissa officinalis L.—leaves), Prickly juniper (Juniperus oxycedrus L.—fruits), Wild camomile (Anthemis cotula L.—flowers), Oats (Avena sativa L.—herb), Coriander (Coriandrum sativum L.—seeds), Flax (Linum usitatissimum L.—seeds) and Marijuana (Cannabis sativa L.—seeds)”. The antioxidant capacities of plant extracts were assayed with four different assays including FRAP, metal chelating, H2O2, DPPH scavenging because the antioxidant capacity cannot be fully described by a single method. The determined flavonoid amounts are between 3.85 and 694-47 (catechin), 2.70 and 2045.49 (epicatechin), 0.12 and 47.11 (rutin), 0.02 and 24.99 (naringin), 0.80 and 153.19 (myricetin), 0.03 and 7.80 (luteolin), 0.02 and 4.75 (naringenin) and 0.38 and 2.73 (apigenin) as µg g−1 plant. These plant materials and their flavonoid components via gamma-aminobutyric acid (GABA) and GABAA receptors can be therapeutical due to their different mechanism on treatment of epilepsy.
Threat analysis and proposed solutions for elekdag wildlife development area
(2017-07-01) Küçük Ö.; Güney K.; Evcin Ö.; Aktürk E.
Natural Wildlife Development Area is a protection status declared in accordance with the Land Hunting Act No. 4915. Within this scope, there are 80 Wildlife Development Areas in our country. The aim of this study was to observe possible threats in the Elekdag wildlife development area and to suggest solutionsfor the area. Elekdag Wildlife Development Area is located in Kastamonu province within the boundaries of Tasköprü county. The administrative responsibility of Elekdag Wildlife Development Area belongs to the General Directorate of National Parks and Nature Conservation, which is affiliated to the Ministry of Environment and Forestry, Kastamonu Provincial Environment and Forestry Directorate. As a result of the study, it was found that the wildlife habitats for deer populations (target species) and water resources in the area at risk. The identification of the risks on habitats and biological resources and proposed solutions have great importance on ecosystem integrity of the protected area. Therefore, we proposed some solutions to eliminate these risks. The most important factor is the arranging on-site management to ensure sustainable protection-use balance.