Browsing by Author "Güney, Kerim"

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Antioxidant enzyme activities and immune responses in rainbow trout (Onchorhynchus mykiss) juveniles fed diets supplemented with dandelion (Taraxacum officinalis) and lichen (Usnea barbata) extracts.
(2021-08-01T00:00:00Z) Salem, Mohamed Omar Abdalla; Salem, Tarek A; Yürüten Özdemir, Keriman; Sönmez, Adem Yavuz; Bilen, Soner; Güney, Kerim
In the present study, antioxidant activity, immune responses, and growth performance of rainbow trout (Onchorhynchus mykiss) juveniles fed with diets supplemented with dandelion (Taraxacum officinalis) and lichen (Usnea barbata) extracts were assessed. Four different concentrations of aqueous methanolic extract of the plants (0% (control), 0.1%, 0.5%, and 1% (D, dandelion; L, lichen) were added to the diets, and fish were fed for 75 days. On the 15, 45, and 75 day of the study, liver antioxidant enzyme activities were determined, and immune responses were determined every 15 day. The results showed that SOD activity increased in the fish group of 0.1% D on the 15 and 45 day compared to control; however, it was lower in all the lichen extract-treated groups than in control at almost all sampling times, except on the 15 day in the 0.1% L group. CAT activity showed an increased value (P < 0.05) in 0.5% L and 1% L treated fish groups on the 15 day, in fish of 1% D and 1% L groups on 45 and on 75 day in 0.1% D group. GPX activity increased on the 15 day of the study in fish of 0.1% D group, on the 45 day in 1% D and 1% L groups and on the 75 day in fish of 0.5% D, 0.1% D, and 0.5% L groups (P < 0.05). G6PDH enhanced in all treatment groups compared to control on the 15 day, except in 0.1% L and 0.5% L groups. An elevated G6PDH activity was also observed on the 75 day of the study in 0.5% D, 1% D, and 0.5% L fish groups. An increase on lipid peroxidation (LP) was observed in all L groups on the 45 day of the study. Lysozyme activity was determined to be the highest in 0.5% and 1% L on the 45 day, in 0.1% L on the 60 day and in the 0.5% L fish group on the 75 day compared to control (P < 0.05). Myeloperoxidase was found to be the highest at the end of the study in 1% L fish group compared to the control (P < 0.05). In conclusion, we suggest the use of dandelion to combat oxidative stress and to lower FCR and the use of lichen to modulate the immune response in rainbow trout. The use of such products will be economical for aquaculture and harmless for the environment.
Contribution to the Flora of Sakarat Mountain (Amasya/Turkey)
(2010-03-01) Cansaran, Arzu; Bingöl, M. Ümit; Geven, Fatmagül; Güney, Kerim; Erdoğan, Neslihan; Kaya, Ömer Faruk KAYA
Bu arastırmada, Sakarat Dağı (Amasya-Türkiye) ve çevresinin floristik özellikleri ve burada yetistiği saptanan damarlı bitki taksonlarının listesi verilmistir. Arastırma 2004-2005 yılları arasında yapılmıstır. Yöreden toplanan 2000 bitki örneğinin değerlendirilmesi ile 78 familyadan 286 cins ve 494 tür ve türaltı seviyede takson saptanmıstır. En büyük familya Astreaceae (70 tür), ikinci Fabaceae (46 tür)’dir. En büyük cinsler ise 8 türle Astragalus L. ve 7’ser türle Veronica L., Bromus L. ve Galium L.’dur. Arastırma alanında fitocoğrafik elementlerin dağılımı ise söyledir; Avrupa-Sibirya 127 (% 25.71), Đran-Turan 43 (% 8.70), Akdeniz 24 (% 4.86), genis yayılıslı veya bilinmeyen 300 (% 60.73). Arastırma alanından toplanan türlerin 40’ı (% 8.1) endemiktir.
Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss).
(2020-02-01T00:00:00Z) Bilen, Soner; Altief, Tarek Abdalsalam Salem; Özdemir, Keriman Yürüten; Salem, Mohamed Omar Abdalla; Terzi, Ertugrul; Güney, Kerim
This study was conducted to determine the effects of dietary supplementation of lemon balm (Melissa officinalis) aqueous methanolic extract on growth performance, blood parameters, digestive and antioxidant enzyme activities, and non-specific immune responses in rainbow trout (Oncorhynchus mykiss). Fish with an average weight of 23.03 ± 0.07 g were fed a diet supplemented with an aqueous methanolic extract of lemon balm at a dose of 0 (control), 0.1 (LB0.1), 0.5 (LB0.5), and 1 g kg (LB1) for 75 days. The final weight, weight gain, and specific growth rate were observed to be significantly increased in LB0.5 and LB1 groups compared with that of the control. No differences were observed in feed conversion ratio values. WBC increased at the 45th day of the study in LB0.1 group. No differences were determined in RBC (P > 0.05). At the end of the study, lipase increased significantly in all experimental groups compared with the control. Pepsin was also elevated in LB0.5 and LB1 groups compared with the control. Increased trypsin was determined in LB1 group (P < 0.05). SOD activity increased at the end of the study in LB1 group (P < 0.05). CAT values had no differences compared with control. GR activity increased in all experimental groups compared with control. GPx improved in LB0.5 and LB1 groups significantly (P < 0.05). Lipid peroxidation was decreased in LB1 group compared with that of control, but this decrease was not significant (P < 0.05). Oxidative radical production and lysozyme activity significantly increased in LB1 group (P < 0.05). The highest MPO was determined in control group (P < 0.05). Current results suggest that lemon balm extract stimulates growth promoting antioxidant and immune responses in rainbow trout.
Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss).
(2021-06-01T00:00:00Z) Bilen, Soner; Ispir, Selcuk; Kenanoglu, Osman Nezih; Taştan, Yiğit; Güney, Kerim; Terzi, Ertugrul
This study investigated the effects of Greek juniper extract on immune responses of rainbow trout. In this experiment, 4 doses [0 (Control), 1 (J1), 4 (J4) and 8 (J8) mg/kg] of the extract were administered orally using an oral gavage twice a day for 14 days. Immune responses were measured on 7th and 14th days. On 14th day, Yersinia ruckeri was injected intraperitoneally to all fish of all groups. On 14th day, ORP in fish of J1 group increased significantly. Lysozyme activity (LA) was increased in J8 group on 7th day (p < .05). On 14th day, a significant decrease was determined in J1 and J4 treatments in LA. Myeloperoxidase activity was significantly decreased in all groups irrespective of sampling times (p < .05). Interleukin (IL)-1β was significantly elevated in fish of J8 group on 7th day. IL-8 increased in fish of J8 and J4 groups on 7th day of the study. IL-12 gene expression was significantly up-regulated in J8 fish group on 7th day, and in J4 fish group on 14th day. Survival rate was higher in J8 treatment compared to the control and other treatments (p < .05). The results suggest that Juniperus excelsa provides protection against Y. ruckeri in rainbow trout.
Prevention of Saprolegniasis in rainbow trout (Oncorhynchus mykiss) eggs using oregano (Origanum onites) and laurel (Laurus nobilis) essential oils.
(2022-01-01T00:00:00Z) Özdemir, Rahmi Can; Taştan, Yiğit; Güney, Kerim
The present study investigated the antifungal effects of essential oils of oregano (Origanum onites) and laurel (Laurus nobilis) on Saprolegniasis, a disease that occurs in rainbow trout eggs during the incubation period. Oregano and laurel were ground after drying, and essential oils were obtained by water distillation method using a Clevenger apparatus. The essential oils were added to potato dextrose agar (PDA) at the rates of 1-1000 ppm, and the minimum inhibitory concentration (MIC) was determined as 250 ppm whereas the minimum lethal concentration (MLC) was determined to be 500 ppm for both plants. In the in vivo trials, fertilized eggs were treated with predetermined doses either by bathing during water hardening and incubation period or only during incubation period, and death rates were monitored during embryological development. The best larvae hatching rate was determined in 500 ppm oregano and 500 ppm laurel groups treated during water hardening plus daily as 82.11% and 79.87%, respectively. According to the results, it was determined that oregano and laurel essential oils exhibited better results in all doses compared with the negative control group, and 500 ppm dose had a better effect than the positive control group treated with formalin.