Browsing by Author "Fazio F."

Now showing 1 - 16 of 16

A comparative evaluation of hematological and biochemical parameters between the italian mullet mugil cephalus (Linnaeus 1758) and the turkish mullet chelon auratus (risso 1810)
(2020-01-01) Fazio F.; Saoca C.; Acar Ü.; Tezel R.; Çelik M.; Yilmaz S.; Kesbiç O.; Yalgin F.; Yiğit M.
This study aimed to carry out a comparative evaluation of the hematological profile (erythrocyte count, hemoglobin concentration, hematocrit, and Wintrobe index parameters such as mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration), in addition to certain biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, serum total protein, albumin, glucose, total cholesterol, and triglycerides), in the Italian mullet Mugil cephalus (Linnaeus 1758) and the Turkish mullet Chelon auratus (Risso 1810). Accordingly, two groups of fish were used in this study: 30 flathead grey mullets, M. cephalus, caught in Lake Faro, Italy, and 30 golden grey mullets, C. auratus, caught in the estuarine channel system of Köyceğiz–Dalyan, Turkey. Statistical evaluations (unpaired t-test) revealed that there were no significant differences in weight or total length values between the two mullet groups. However, the differences between all the evaluated blood parameters (except alanine aminotransferase) between the Italian and the Turkish mullet were significant (P < 0.0001). The present study demonstrated that there were significant hematological and biochemical differences between the Italian (M. cephalus) and the Turkish (C. auratus) mullet. Our findings also contribute to expanding the knowledge on the hematology and biochemistry of two different species of mullet originating from two different habitats. Since the evaluation of blood parameters represents an essential tool in examining the effects of environmental conditions on fish physiology and, consequently, on fish health status, further research in this field is encouraged and would be very useful.
Antioxidant Performance of Borago officinalis Leaf Essential Oil and Protective Effect on Thermal Oxidation of Fish Oil
(2023-07-01) Hasdemir Ö.; Kesbiç O.S.; Cravana C.; Fazio F.
Antioxidant Performance of Borago officinalis Leaf Essential Oil and Protective Effect on Thermal Oxidation of Fish Oil
(2023-07-01) Hasdemir Ö.; Kesbiç O.S.; Cravana C.; Fazio F.
Comparative study of some hematological and biochemical parameters of italian and Turkish farmed rainbow trout Oncorhynchus mykiss (Walbaum, 1792)
(2016-01-01) Fazio F.; Saoca C.; Piccione G.; Kesbiç O.S.; Acar Ü.
Blood parameter assessments of fish may be used as quick tools for diagnosing health status. The aim of this research was to undertake a comparative study of hematological and biochemical parameters of Italian and Turkish farmed rainbow trout Oncorhynchus mykiss (Walbaum, 1792) to establish baseline values in relation to different areas. Forty Italian trout (32.59±0.25 cm total length, 397.40±6.49 g weight) and forty Turkish trout (33.00±0.24 cm total length, 385.70±3.50 g weight) were examined. Statistical analysis (unpaired t-test) showed no significant differences in weight, length and condition factor between two farmed trout groups. Statistically significant differences (P < 0.05) were found in some hematological and biochemical parameters between Italian and Turkish farmed rainbow trout. Our findings shown that in Italian farmed rainbow trout Red blood cell (RBC), Hematocrit (Hct), Cholesterol and Total protein values were significantly lower than Turkish trout, instead Mean corpuscular volume (MCV), Mean corpuscular hemoglobin (MCH), Mean corpuscular hemoglobin concentrations (MCHC), Glucose, Triglycerides and Serum albumin shown significantly higher values. No statistically significant differences were found for White blood cell (WBC) and hemoglobin (Hgb). The results of this study contribute to knowledge of the baseline of hematological and biochemical parameters of rainbow trout farmed in two different habitats suggesting that blood parameters could be an important value in monitoring environmental conditions on fish physiology and fish culture management.
Comparative study on haematological and biochemical parameters of two wild sparid fish species
(2019-01-01) Acar Ü.; Saoca C.; Kesbiç O.; Yilmaz S.; Yigit M.; Inanan B.; Fazio F.
Different feeding habits may affect the blood parameters in fish species of the same family, even if they live in the same marine habitat. The aim of this study was to compare some haematological and biochemical parameters of two marine fish species of the same family, the saupe Sarpa salpa (Linnaeus, 1758) and the common two-banded seabream Diplodus vulgaris (Geoffroy Saint-Hilaire, 1817), and to establish the similarities and differences between these species. For this purpose red blood cells (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), glucose (GLU), triglycerides (TRIG), cholesterol (CHOL), serum total protein (TP), albumin (ALB), globulins (GLO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were determined for 30 fish from each species. Statistical analysis showed significant differences in some haematological and biochemical (RBC, Hb, Hct, TP, ALB, GLO, AST, ALT, ALP and LDH) parameters between the two species. Lower values of RBC, Hb and Hct were found in S. salpa in comparison with D. vulgaris. These differences could be attributed to the feeding behavior, life style and habitat adaptation of the different fish species of the same family and contribute to knowledge of reference values of blood parameters in Sarpa salpa and Diplodus vulgaris. No significant differences were found in MCV, MCH, MCHC and GLU, TRIG, CHOL.
Correction to: Effects of heavy pollution in different water bodies on male rainbow trout (Oncorhynchus mykiss) reproductive health (Environmental Science and Pollution Research, (2022), 30, 9, (23467-23479), 10.1007/s11356-022-23670-w)
(2023-02-01) Bhat R.A.; Saoca C.; Cravana C.; Fazio F.; Guerrera M.C.; Labh S.N.; Kesbiç O.S.
The images of Figs. 3 and 4 should be interchanged. The Original article has been corrected.
Effect of Different Anaesthetics on Hematology and Blood Biochemistry of Labeo rohita
(2024-01-01) Habib S.S.; Naz S.; Batool A.I.; Rehman M.F.U.; Ullah M.; Kesbiç O.S.; Maricchiolo G.; Fazio F.
The current study aims to determine the effect of four different anesthetics such as MS-222 (100 mg/L), tobacco extract (50 mg/L), propiscin (1.0 ml/L) and clove oil (40 mg/L) on the hematology and blood biochemical parameters of Labeo rohita. Blood was sampled 10 minutes after the anesthesia was administered and again after 24 hours. Except for the clove oil 24h measurements, there was a significant difference in the number of blood cells in all anesthetic applications (P<0.05). Hemoglobin and hematocrit values were not altered after 24 hours of treatment with clove oil and propiscin. MCV, MCH and MCHC values were not significantly affected in any application except clove oil 24h. Biochemical parameters, including total protein and glucose level, significantly changed (P<0.05) in propiscin and clove oil group. Enzymatic activities such as ALT changed significantly with MS-222, and AST values were non-significant (P>0.05) with tobacco, Change has been detected in ALP with MS-222 and tobacco calcium and magnesium showed non-significant differences with anesthetics. Overall all the parameters were affected by anesthetics used in the current research, however, it was established that 24 hours following the administration of clove oil, the discrepancies in blood values had vanished to a great extent.
Effects of different levels of pomegranate seed oil on some blood parameters and disease resistance against Yersinia ruckeri in rainbow trout
(2018-05-23) Acar Ü.; Parrino V.; Kesbiç O.S.; Lo Paro G.; Saoca C.; Abbate F.; Yilmaz S.; Fazio F.
This study is aimed to assess the effects of pomegranate seed oil (PSO) supplementation on growth performance, some hematological, biochemical and immunological parameters, and disease resistance against Yersinia ruckeri in cultured rainbow trout Oncorhynchus mykiss (Walbaum, 1792). 240 fish in total were randomly assigned into four triplicate groups (20 fish/per aquarium) corresponding to four dietary treatments: control (PSO0; no addition of PSO), 0.5% (PSO5), 1.00% (PSO10), and 2.00% (PSO20) of PSO, respectively. After the 60 day-feeding trial, fish blood samples were collected and compared. Statistical analysis (one-way ANOVA) showed a significant (P < 0.05) effect of PSO on red blood cell count, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin concentration, cholesterol, aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase parameters in PSO5 and PSO10 with regard to control. Moreover, a pronounced (P < 0.05) increased in weight gain, growth and feed conversion was found in fish fed with PSO supplemented diets. After the feeding trial, fish were challenged with Y. ruckeri and survival recorded for 20 days. Cumulative survival was 45.10% in fish fed with the control diet, whereas in fish fed with PSO5, PSO10, and PSO20 supplemented diets, survival was 58.82, 56.86, and 56.86%, respectively. In conclusion, dietary administration of PSO induced a reduction in mortality of rainbow trout infected with Y. ruckeri, intercalary significant differences occurred on growth performance and some blood values among treated groups. These positive effects of PSO could be considered for new applications in aquaculture.
Effects of essential oil derived from the bitter orange (Citrus aurantium) on growth performance, histology and gene expression levels in common carp juveniles (cyprinus carpio)
(2021-05-01) Acar Ü.; Kesbiç O.S.; Yılmaz S.; İnanan B.E.; Zemheri-Navruz F.; Terzi F.; Fazio F.; Parrino V.
The aim of this study was to detect effects of bitter orange (Citrus aurantium) essential oilcommonly called neroli oil (NO) (0, 0.25, 0.50, 1, and 1.5% referred to as NO0 NO0.25, NO0. 05, NO1 and NO1.5, respectively) on growth performance output and expression levels of some growth-related genes in the muscle tissue and some immune-related genes in the head kidney and pathologicadifferences in digestive system organs of common carp Cyprinus carpio. The NO0.25 group had a large improvement in growth efficiency at the end of the 60-day feeding cycle. Real-time PCR (Bio RAD, USA) system was used to detect variations in gene expression levels. Furthermore, NO supplementation of up to 0.25% in muscle tissue controlled the release of growth hormone (GH) and insulin-like growth factor I (IGF-I). Furthermore, in the NO0.25 treatment category, immune response gene levels TNF-α, IL-8 and IL-1ß increased in head kidney tissue. In the histological examination of the liver and intestine, there were significant differences between fish fed with N1 and N1.5 dietsThis study confirms that dietary supplementation of NO up to 0.25% can improve common carp growth efficiency and increase the expression of genes (GH and IGF-I) related to muscle growthTNF-α, IL-8 and IL-1ß genes related to immune status, and liver and intestine histological status ocommon carp.
Effects of heavy pollution in different water bodies on male rainbow trout (Oncorhynchus mykiss) reproductive health
(2023-02-01) Bhat R.A.; Saoca C.; Cravana C.; Fazio F.; Guerrera M.C.; Labh S.N.; Kesbiç O.S.
This research aimed to investigate the influence of heavy metals on the reproductive health of rainbow trout (Oncorhynchus mykiss) captured from two locations, Verinag (S1) and Panzath (S2). Sixty (n. 60) mature rainbow trout samples (30 from each site) with body weights ranging from 400 to 650 g were collected from Verinag hatchery, which was less polluted (S1), and Panzath hatchery, which was more polluted (S2). The findings revealed significant differences between the two sites, S1 (less polluted) and S2 (more polluted), as well as significant variations within the tissues. Iron (Fe) and Zinc (Zn) were found to be highest in sampling water as well as in fish tissues sampled from S2 sites. Similarly, concerning S1 captured fishes, histopathological examination of testes from S2 captured fish was found. Testicular abnormalities that included disorganization of the seminiferous tubules, reduction in the number of germ cells (sperm cells, spermatozoa), vacuolization, and large empty areas in the seminiferous epithelium were found. In testicular cells, the frequency of apoptotic cells collected from S2 water increased significantly (P < 0.05). SOD, catalase, and glutathione peroxidase activity increased in S1 captured fishes but decreased in S2 captured fishes. MDA levels gradually increased in S2 captured fish, and the degree of heavy metal stress was positively correlated (P < 0.05). In male rainbow trout, testosterone and 11-ketotestosterone levels increased substantially in S1 captured fishes. While the fatty acids of testes in S2 fishes decreased with respect to S1 fishes. In conclusion, S2 captured fish suffered more damage due to heavy metals including cellular damage, apoptosis, oxidative damage, and altered steroid hormones when compared to fish from S1 waters.
Effects of Monterey Cypress (Cupressus macrocarpa Hartw) Leaf Essential Oil as a Dietary Supplement on Growth Performance and Haematological and Biochemical Parameters of Common Carp (Cyprinus carpio L.)
(2020-10-01) Kesbiç O.S.; Parrino V.; Acar Ü.; Yilmaz S.; Paro G.L.; Fazio F.
Common carp (Cyprinus carpio) is the most farmed freshwater fish worldwide. In recent years, use of natural products in fish diets has become popular in aquaculture, to improve fish health and growth performance. The present study investigated the effects of essential oil from the leaves of Monterey cypress (Cupressus macrocarpa; CMEO) on growth performance and blood parameters in common carp fingerlings. Identification of 96.1% of the CMEO total volatile components was achieved, with the highest contents for terpinen-4-ol and α-pinene, at 22.9% and 47.7%, respectively. After 60 days of feeding of the fingerlings with supplemented diets without CMEO (CMEO 0%) and with CMEO at 0.5%, 0.75% and 1%, the best growth performance was seen for those fish fed with the CMEO 0.5% diet. No significant differences were seen for the haematological parameters and blood cell indices versus CMEO 0%. Serum glucose, triglycerides, cholesterol and glutamic pyruvic transaminase were significantly reduced in the fingerlings fed with the CMEO 0.5% diet versus CMEO 0%. Thus, CMEO oil as a 0.5% dietary supplement can be used to improve the growth performance and health status of the common carp without any adverse effects seen.
Effects of Tomato Paste By-Product Extract on Growth Performance and Blood Parameters in Common Carp (Cyprinus carpio)
(2022-12-01) Kesbiç O.S.; Acar Ü.; Hassaan M.S.; Yılmaz S.; Guerrera M.C.; Fazio F.
This research examined different growth performances and blood parameters of common carp (Cyprinus carpio) consuming diets supplemented with tomato paste by-product extract (TPE). Five diets with different concentrations of TPEs, 0% (TPE0) 0.5% (TPE0.5), 1% (TPE1), 2% (TPE2), and 5% (TPE5) were supplied to 300 common carp (8.38 ± 0.18 g) (60 fish per group) each day during the 60-day feeding trial. The relative and specific growth rates of fish-fed supplemental diets increased significantly, while the feed conversion ratio significantly decreased (p < 0.05). TPE considerably increased the erythrocyte count (RBC), hemoglobin content (Hb), and hematocrit (Hct) (p < 0.05), according to hematological analyses of blood samples collected after the feeding experiment. The blood biochemical findings indicate that using 1% or more extracts considerably reduced the serum glucose, cholesterol, and triglyceride ratios while significantly increasing the total protein, albumin, and globulin ratios in common carp (p < 0.05). Based on the findings of the study, it was concluded that the 2% extract generated from tomato paste by-products in common carp diets could be utilized as a growth-promoting product without any negative effects on blood parameters linked to feeding in carp.
Evaluation of an innovative and sustainable pre-commercial compound as replacement of fish meal in diets for rainbow trout during pre-fattening phase: Effects on growth performances, haematological parameters and fillet quality traits
(2021-12-01) Acar Ü.; Giannetto A.; Giannetto D.; Kesbiç O.S.; Yılmaz S.; Romano A.; Tezel R.; Türker A.; Güllü K.; Fazio F.
The aim of the study was to determine the potential and sustainable use of pre-commercial product ITTINSECT™ APS V1 as a major protein source in rainbow trout (Oncorhynchus mykiss) diets. A 60-day feeding experiment was conducted to potentially use ITTINSECT as fish meal replacement in the diets of rainbow trout. Five isonitrogenous in dry matter (38% crude protein) and isolipidic (15% crude lipid) diets were produced: a control diet (fishmeal-based) (ITT0) and four experimental diets replacing fishmeal by 25 (ITT25), 50 (ITT50), 75 (ITT75) and 100 (ITT100) %, with ITTINSECT™ APS V1. Triplicate tanks, containing 15 fish each (65.81 ± 1.26 g), were hand-fed to apparent satiation twice every day during the experiment. At the end of the feeding trial, significantly higher growth performance was observed in the group fed ITTM25 and ITTM50 diets. This performance was supported by growth-related gene expressions analyzed in muscle; significantly higher GH and IGF-I genes expression levels were determined in ITT25 and ITT50 when compared to control (ITT0) (p < 0.05). While no significant differences were found between the hematology values (p > 0.05), serum total protein, globulins and glucose levels were significantly different between experimental groups (p < 0.05). In addition to this, the immune-related genes such as TNF-α, IL8 and IL1-β expression levels were determined to be significantly different (p < 0.05). In conclusion, in order to achieve the best growth performance in rainbow trout and enhance sustainable aquaculture practices, replacement of fish meal with up to 50% ITTINSECT™APS V1 in diets for rainbow trout is suggested..
Hot pepper (Capsicum sp.) oil and its effects on growth performance and blood parameters in rainbow trout (Oncorhynchus mykiss)
(2020-11-16) Parrino V.; Kesbiç O.S.; Acar Ü.; Fazio F.
The authors studied the effect of hot pepper (capsicum sp.) oil on the growth performance and blood parameters in rainbow trout fed. Hot pepper oil was added to rainbow trout feeds at the rates of HPO 0‰ (0 mg/kg) (control), HPO 1‰ (1 mg/kg), HPO 2‰ (2 mg/kg), HPO 4‰ (4 mg/kg) and HPO 6‰ (6 mg/kg), and the fish were fed with experimental feeds for 60 days. The group fed with HPO 4‰ showed the highest percentage growth rate and the lowest feed conversion rate. Our results showed the significant differences serum biochemical parameters, a decrease of serum liver enzymes, glucose, cholesterol and triglyceride levels and an increase of total protein and albumin levels compared with the control. The use of HPO 1‰ in rainbow trout showed a positively affects the growth performance, haematological and serum biochemical parameters. (Figure presented.).
Lipid and Protein Content in Rainbow Trout in Relation to Gonadal Growth
(2022-12-01) Bhat R.A.; Saoca C.; Arfuso F.; Pino C.; D'Alessandro E.; Zumbo A.; Fazio F.; Kesbiç O.S.
The purpose of this study was to examine various lipid classes as well as the protein content in rainbow trout ovaries. A total of 80 samples of Oncorhynchus mykiss (Walbaum 1792) were collected from Kokernag and Verinag hatcheries (Jammu and Kashmir, India) between 2017 and 2019. The mature stage in female fish had the highest gonadosomatic index (GSI) and minimal GSI in the spent stage. Higher lipid content of the ovary was reported during the mature stage (36%), as compared to other pre-breeding stages. Similarly, lipid classes also showed fluctuation during the development of the ovary. The lipid classes, i.e. glycolipids, free fatty acids, cholesterol and triglycerides, had a higher content in the mature stage, while the phospholipid content was higher in the mature stage. The protein content was also higher in the mature ovary than in the other developmental stages. The study provides reference values for various biochemical parameters in rainbow trout that could be useful for population monitoring programs and for the development of diets and management methods for fish production under controlled conditions.
Toxic effect of heavy metals on ovarian deformities, apoptotic changes, oxidative stress, and steroid hormones in rainbow trout
(2023-01-01) Bhat R.A.; Bakhshalizadeh S.; Guerrera M.C.; Kesbiç O.S.; Fazio F.
Background: As is well known, the pollution in the aquatic environment in which fish grow has a direct impact on aquaculture practices. Pollution in aquatic systems because of multiple adverse effects on fish metabolic processes, especially the reproductive systems. Aim: The goal of this study was to assess the severity of pollution impact in two different hatcheries, Verinag hatchery, Site 1 (S1) and Panzath hatchery, Site 2 (S2) in Anantnag region, using histopathological, ultrastructural, oxidative stress, genotoxic, and hormonal analysis in rainbow trout gonad (ovary). M&M: Fish were collected between May 2018 and April 2019 from two locations, Verinag hatchery (S1) and Panzath hatchery (S2), which were affected by heavy metals. Results: The histological and ultrastructural examination of rainbow trout ovaries from the Verinag hatchery (S1) revealed normal structure in growing oocytes in rainbow trout at various stages based on morphological features while the fish ovaries in the Panzath hatchery (S2) showed various deformities and irregularly shaped oocytes. The surfaces of some of these oocytes were wrinkled, rough, or distorted. Apoptotic studies revealed that the frequency of apoptotic cells collected from S2 water was significantly increased in ovarian cells (P < 0.05). The activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were found to be increased in fish collected from S1 but decreased in fish collected from S2. In S2 caught fish, malondialdehyde (MDA) levels were found to increase gradually, and the degree of heavy metal stress was positively correlated (p < 0.05). The comet assay was used to determine the induction of DNA damage in ovarian cells. The induction of DNA damage was found to be significantly higher (p < 0.05) in S2 fish specimens compared to fish from S1. On comparing the DNA damage of the rainbow trout from the two sampling sites, it was revealed that the fish is much more sensitive to aquatic contaminants. Regarding steroid hormones, higher levels of progesterone and estrogen were reported in the fish samples collected from S1 as compared to S2 captured fish. Conclusion: In conclusion, the comparative study of fish from two different sites viz. Verinag hatchery (S1) and Panzath hatchery (S2) revealed that S2 sampled fish suffered more heavy metal damage, including cellular deformities, apoptosis, oxidative damage, and altered steroid hormones.