Browsing by Author "Evans H.C., Dinh T.T.N., Ugur M.R., Hitit M., Sajeev D., Kaya A., Topper E., Nicodemus M.C., Smith G.D., Memili E."
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Publication Lipidomic markers of sperm cryotolerance in cattle.(2020-11-19T00:00:00Z) Evans, Holly C, Dinh, Thu T N, Ugur, Muhammet Rasit, Hitit, Mustafa, Sajeev, Dishnu, Kaya, Abdullah, Topper, Einko, Nicodemus, Molly C, Smith, Gary D, Memili, Erdogan; Evans, HC, Dinh, TTN, Ugur, MR, Hitit, M, Sajeev, D, Kaya, A, Topper, E, Nicodemus, MC, Smith, GD, Memili, EThe objective of the current study was to determine the fatty acid composition of sperm from Holstein bulls with different freezability (Good and Poor; n = 12). Fatty acids were extracted from frozen sperm in 1:2 (v/v) chloroform-methanol solvent, fractionated into neutral and polar fractions, and composition determined by gas chromatography-mass spectrometry. Thirty-four fatty acids were quantified and their concentrations and percentages within each lipid fraction were calculated. Overall, saturated fatty acids (SFA) were predominant, accounting for 71 to 80% of fatty acids in neutral and polar lipid factions. There were marked differences in fatty acid composition between the lipid fractions (P < 0.001). The branched chain fatty acid (BCFA) concentration (15 to 18 µg) was almost twice as much as polyunsaturated fatty acids (PUFA) concentration found in the polar lipid fraction (8 to 9 µg; P < 0.001). Sperm with different freezability phenotypes only had a few differences in 22:0, 18:1 cis 9, and 14:0 13-methyl fatty acids (P ≤ 0.011). These results are significant because they reveal key understandings of fatty acid composition of sperm membrane and lay a foundation for the manipulation of membrane integrity, fluidity, and stability to advance the assisted reproductive technologies.