Browsing by Author "Demirkol D.O."
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Scopus Comparison of direct and sandwich type immunoassays on electrospun nanofibers using of metal organic frameworks as a fluorescence probe(2022-12-01) Kirbay F.O.; Yazgan İ.; Demirkol D.O.Here, colloidal fluorescent Fe (III) metal organic frameworks (FeMOFs) were synthesized using FeCl3 as Fe3+ ion source and lactose 1-aminonaphthalene (L1AN) as an organic linker by one-step synthesis approach. C-reactive protein antibody (Anti-CRP) was conjugated to L1AN-FeMOF through amide bond formation, which was then applied to the detection of C-reactive protein (CRP) using sandwich-type and immunometric assays. To cover 96-well plate surfaces, polycaprolactone and poly (amic acid) (PCL/PAA) electrospun nanofibers were synthesized. Limit of detection (LOD) for CRP was obtained as 4.8 pg/mL for the sandwich assay and 9.0 pg/mL for the immunometric assay in buffer systems using high throughput sensing approach. Real sample applications in artificial saliva and simulated serum validated the reliability of the approach. Selectivity control tests were performed using common serum-based interferents, for which minimum to no interference was observed. Based on the findings, the developed assay can be offered as a new approach in the detection of CRP in clinical samples.Scopus Laccase assay based on electrochemistry and fluorescence detection via anthracene sequestered poly(amic acid) films(2018-10-01) Esen E.; Yazgan I.; Demirkol D.O.; Timur S.Phenols are among the most problematic organic compounds, which manipulates metabolism of living organisms and pose threat to environment. Detection of total phenol at the lowest allowable concentration is critical, so development of sensitive and easy to use methods have been heavily investigated. Hereby, for the first time, anthracene (Ant) was sequestered into poly(amic acid) (PAA) to form fluorescent and conductive film support for laccase (Lac) immobilization to quantify total phenol content at high specificity and selectivity. Ant-PAA/Lac films were applied to fluorescent and electrochemical quantification of phenol, which provided high sensitivity (LOD 46 μM) and reproducibility (RSD, 0.5%) for the tested 10 phenolic compounds including phenol, catechol, 3-acetominophenol, hydroquinone, L-DOPA, 2,6-dimethylphenyl, syringalzadine, 3,5-dimethoxy-4-hydroxycinnamic acid, 2,5-dimethoxyaniline and guaiacol. The sensor system showed strong rejection to common organic interferent while real sample application revealed the applicability of the sensor. Therefore, the results can call the sensor system, particularly fluorescence-based quantification in 96-well plate, is an alternative approach for high-throughput screening of total phenolic compounds in waste-water at cost-effective manner.