Browsing by Author "Demirci B."

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Analysis of the essential oils of subgenus Caropodium from Turkey; Grammosciadium schischkinii (V.M.Vinogr. & Tamamsch.) V.M.Vinogr., G. haussknechtii Boiss., G. pterocarpum Boiss. and G. platycarpum Boiss. & Hausskn. ex Boiss.
(2017-03-04) Küçükboyacı N.; Demirci B.; Ayaz F.; Bani B.; Adıgüzel N.
The composition of the hydrodistilled essential oils obtained from aerial parts of Grammosciadium schischkinii (V.M.Vinogr. & Tamamsch.) V.M.Vinogr., G. haussknechtii Boiss., G. pterocarpum Boiss. and G. platycarpum Boiss. & Hausskn. ex Boiss. (Apiaceae) which belong to subgenus Caropodium, growing in Turkey, were simultaneously analyzed by GC and GC-MS. The analysis revealed fifty-seven constituents, accounting 84.3–99.5% of the oils. The essential oil composition of G. schischkinii and G. haussknechtii which are endemic taxa to Turkey, have herein demonstrated for the first time. Moreover, we firstly defined the chemical characterization of the essential oil obtained from G. platycarpum gathered from Turkey. Oil samples showed different chemical compositions with regard to species and locations. Linalool (88.5 and 94.2%) was the main component of the oil samples from G. platycarpum, while hexadecanoic acid (20.6%) was the major in the oil sample of G. haussknechtii. Caryophyllene oxide (45.9 and 20.7%) was defined as the principal constituent in the oil samples of G. schischkinii and G. pterocarpum, respectively. In addition, phytol (19.1%) was determined as the main component in the oil of G. haussknechtii and this compound was first determined in the highest amount among the essential oils of the genus Grammosciadium.
Composition of the essential oils of the subgenus Grammosciadium from Turkey; G. Confertum, G. cornutum, G. macrodon subsp. macrodon, G. macrodon subsp. nezaketae and G. daucoides
(2016-09-01) Küçükboyacı N.; Demirci B.; Ayaz F.; Bani B.; Adıgüzel N.
Essential oils obtained by hydrodistillation from the aerial parts and fruits of five taxa of genus Grammosciadium DC., belonging to subgenus Grammosciadium (G. confertum Hub.-Mor. & Lamond, G. cornutum (Nábělek) C.C.Towns., G. macrodon Boiss. subsp. macrodon, G. macrodon Boiss. subsp. nezaketae B.Bani and G. daucoides DC.), collected from different locations in Turkey, were simultaneously analyzed by GC and GC/MS systems. 124 components representing 71.1-99.8% of the total contents were identified in the oils. Oil samples from fruits and aerial parts of the plants showed different chemical profiles with regard to species. We have herein demonstrated that the chemical composition of essential oil samples from G. cornutum, G. macrodon subsp. macrodon, G. macrodon subsp. nezaketae (an endemic subspecies), and G. confertum (an endemic species) growing in Turkey was determined for the first time. Hexadecanoic acid (13.3-21.2% and 48.1-59.8%) was the main component of the samples of G. cornutum and G. confertum, respectively, while caryophyllene oxide (13.1-29.2%) was the major constituent in the samples of G. macrodon subsp. nezaketae and G. macrodon subsp. macrodon as well as γ-terpinene (61.9%) and carvacrol (68.9%) in G. daucoides samples. In addition, pentacosane can be considered a chemotaxonomic marker for the essential oil of G. macrodon subsp. macrodon.
Dietary effect of grape (Vitis vinifera) seed extract mitigates hepatic disorders caused by oxidized fish oil in rainbow trout (Oncorhynchus mykiss)
(2023-01-01) Terzi F.; Demirci B.; Acar Ü.; Yüksel S.; Salum Ç.; Erol H.S.; Kesbiç O.S.
The major goal of this study was to determine the effect of grape seed extract (GSE) on liver damage in rainbow trout (Oncorhynchus mykiss) that was caused by the consumption of dietary oxidized fish oil (OFO). Rainbow trout were fed six different experimental diets coded OX-GSE 0 (OFO diet), OX-GSE 1 (OFO and 0.1% GSE), OX-GSE 3 (OFO and 0.3% GSE), GSE 0 (fresh fish oil and 0.0% GSE), GSE 1 (fresh fish oil and 0.1% GSE), and GSE 3 (fresh fish oil and 0.3% GSE) for 30 days. The lowest % hepatosomatic index (HSI) result was calculated in fish fed with OX-GSE 0 and the highest HSI was determined in fish fed with GSE 1 diets (p < 0.05). Histopathologically, hydropic degeneration in hepatocytes significantly increased OX-GSE 0 and GSE 3 compared to GSE 1 diets (p < 0.05). Deposition of lipid droplets in hepatocytes was significantly increased in OX-GSE 0 and OX-GSE 3 groups than others (p < 0.05). Liver biochemistry parameters such as superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) were significantly affected by OX and GSE treatments (p < 0.05). There were significant differences in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) among the liver enzymes analyzed in serum in OX and GSE (p < 0.05), meanwhile no difference was observed in lactate dehydrogenase (LDH) values between groups (p > 0.05). In conclusion, liver biochemistry and histopathology of rainbow trout consuming diets containing oxidized fish oil were negatively affected. However, it was determined that the supplementation of 0.1% GSE to the diet had a significant ameliorative role in these adverse effects.
Does dietary incorporation level of pea protein isolate influence the digestive system morphology in rainbow trout (Oncorhynchus mykiss)?
(2021-11-01) Demirci B.; Terzi F.; Kesbic O.S.; Acar U.; Yilmaz S.; Kesbic F.I.
In the present study, fish meal (FM) was replaced by pea (Pisum sativum) protein (PP) in diet for Rainbow trout (Oncorhynchus mykiss) at levels of 0% (PP0), 25% (PP25), 50% (PP50), 75% (PP75) and 100% (PP100), and the effect of dietary PP level on the digestive system tracts and liver was investigated by micromorphological and histopathological evaluations. Morphometric measurements (mm 100g fish−1) of the liver width and stomach length in rainbow trout were found to be significantly larger (p <0.05) in fish with high-level pea protein as the main protein source (PP75, PP100) compared to the low-level PP replacement group (PP25). No significant differences were found in morphometric measurements for pyloric caecum and intestines among treatment groups, whereas the number of the caecum of fish fed the PP25 diets significantly increased over the control (PP0) (p<0.05). In the histological examination of the liver, mild hydropic and vacuolar degeneration was observed in all experimental groups except PP0 and PP25. The measurements of pyloric caecum fold height, enterocyte length and width of tunica muscularis of the high-level pea protein groups of PP75 and PP100 were significantly higher (p <0.05) compared to the control group. In conclusion, 25% substitution of PP can be suggested for FM in trout diets, because the findings of the present study provided evidence that the digestive system improved by increasing the number of pyloric caecum at this replacement level.
Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS
(2022-12-01) Terzi F.; Demirci B.; Çınar İ.; Alhilal M.; Erol H.S.
Background: Acute respiratory distress syndrome (ARDS) is a life-threatening disease caused by the induction of inflammatory cytokines and chemokines in the lungs. There is a dearth of drug applications that can be used to prevent cytokine storms in ARDS treatment. This study was designed to investigate the effects of tocilizumab and dexamethasone on oxidative stress, antioxidant parameters, and cytokine storms in acute lung injury caused by oleic acid in rats. Methods: Adult male rats were divided into five groups: the CN (healthy rats, n = 6), OA (oleic acid administration, n = 6), OA + TCZ-2 (oleic acid and tocilizumab at 2 mg/kg, n = 6), OA + TCZ-4 (oleic acid and tocilizumab at 4 mg/kg, n = 6), and OA + DEX-10 (oleic acid and dexamethasone at 10 mg/kg, n = 6) groups. All animals were euthanized after treatment for histopathological, immunohistochemical, biochemical, PCR, and SEM analyses. Results: Expressions of TNF-α, IL-1β, IL-6, and IL-8 cytokines in rats with acute lung injury induced by oleic acid were downregulated in the TCZ and DEX groups compared to the OA group (P < 0.05). The MDA level in lung tissues was statistically lower in the OA + TCZ-4 group compared to the OA group. It was further determined that SOD, GSH, and CAT levels were decreased in the OA group and increased in the TCZ and DEX groups (P < 0.05). Histopathological findings such as thickening of the alveoli, hyperemia, and peribronchial cell infiltration were found to be similar when lung tissues of the TCZ and DEX groups were compared to the control group. With SEM imaging of the lung tissues, it was found that the alveolar lining layer had become indistinct in the OA, OA + TCZ-2, and OA + TCZ-4 groups. Conclusions: In this model of acute lung injury caused by oleic acid, tocilizumab and dexamethasone were effective in preventing cytokine storms by downregulating the expression of proinflammatory cytokines including TNF-α, IL-1β, IL-6, and IL-8. Against the downregulation of antioxidant parameters such as SOD and GSH in the lung tissues caused by oleic acid, tocilizumab and dexamethasone upregulated them and showed protective effects against cell damage.
Gill arch and raker morphology of common carp (Cyprinus carpio, Linnaeus, 1758) sampled in aquaculture system
(2023-01-01) Demirci B.; Kesbiç O.S.
Gossypin mitigates oxidative damage by downregulating the molecular signaling pathway in oleic acid-induced acute lung injury
(John Wiley and Sons Ltd, 2023) Dincer B.; Cinar I.; Erol H.S.; Demirci B.; Terzi F.
One of the leading causes of acute lung injury, which is linked to a high death rate, is pul-monary fat embolism. Increases in proinflammatory cytokines and the production of freeradicals are related to the pathophysiology of acute lung injury. Antioxidants that scav-enge free radicals play a protective role against acute lung injury. Gossypin has beenproven to have antioxidant, antimicrobial, and anti-inflammatory properties. In this study,we compared the role of Gossypin with the therapeutically used drug Dexamethasonein the acute lung injury model caused by oleic acid in rats. Thirty rats were divided intofive groups; Sham, Oleic acid model, Oleic acid+Dexamethasone (0.1 mg/kg), Oleic acid+Gossypin (10 and 20 mg/kg). Two hours after pretreatment with Dexamethasone orGossypin, the acute lung injury model was created by injecting 1 g/kg oleic acid into thefemoral vein. Three hours following the oleicacid injection, rats were decapitated. Lungtissues were extracted for histological, immunohistochemical, biochemical, PCR, andSEM imaging assessment. The oleic acid injection caused an increase in lipid peroxidationand catalase activity, pathological changes in lung tissue, decreased superoxide dismu-tase activity, and glutathione level, and increased TNF-α,IL-1β, IL-6, and IL-8 expression.However, these changes were attenuated after treatment with Gossypin and Dexameth-asone. By reducing the expression of proinflammatory cytokines and attenuating oxida-tive stress, Gossypin pretreatment provides a new target that is equally effective asdexamethasone in the treatment of oleic acid-induced acute lung injury
Morphological structure of rat tongue using light and scanning electron microscopy
(2023-01-01) Demirci B.; Kandil B.; Yüksel S.; Gültiken M.E.
The rat is one of the most commonly used animals in biological research and experimental investigations in medicine. The ultrafine structural components of the epithelium differ depending on the anatomy of the animal and the papilla type. Animal adaptation to food types and environmental circumstances may also be linked to morphological diversity. In the current study, seven male Wistar rat tongues were investigated. For scanning electron microscope (SEM), two rat tongues were immersed in a 10% formalin solution and the other two rat tongues were immersed in a 2.5% glutaraldehyde solution. The tongues of three rats were fixed for regular histological evaluation using triple staining. The three primary components of the Wistar rat tongue are the apex, body and root. The apex had a rounded and bifurcated shape. Filiform papillae and gustatory papillae were easily identified on the dorsal side of the tongue. There were three forms of gustatory papillae; fungiform papillae, vallate papillae and foliate papillae. The purpose of this study was to expose the tongue morphology of the Wistar rat species, which is widely used in investigations. Also, we wanted to show that formalin fixation can be utilized for morphological research in SEM. Finally, the Wistar rat tongue was thoroughly investigated and compared to those of other species.
Volatile compounds from the aerial part and fruits of Grammosciadium pterocarpum Boiss. Growing in Turkey
(2015-05-04) Küçükboyaci N.; Demirci B.; Adigüzel N.; Bani B.; Başer K.H.C.
Volatile constituents obtained from crushed fruits and aerial parts of Grammosciadium pterocarpum Boiss. (Apiaceae) by microdistillation were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS) systems, simultaneously. Fifteen components comprising 99.5% of the volatile oil of the fruit and twenty components comprising 97.4% of the volatile oil of the aerial parts were identified. The main constituents of the fruit volatiles were found to be linalool (68.4%) and β-pinene (22.0%), whereas caryophyllene oxide (55.1%) and β-caryophyllene (15.3%) were found to be major constituents in the volatiles of the aerial parts.