Browsing by Author "Demirci, B."

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    Web of Science
    Gossypin mitigates oxidative damage by downregulating the molecular signaling pathway in oleic acid-induced acute lung injury
    (2023.01.01) Dincer, B.; Cinar, I.; Erol, H.S.; Demirci, B.; Terzi, F.
    One of the leading causes of acute lung injury, which is linked to a high death rate, is pulmonary fat embolism. Increases in proinflammatory cytokines and the production of free radicals are related to the pathophysiology of acute lung injury. Antioxidants that scavenge free radicals play a protective role against acute lung injury. Gossypin has been proven to have antioxidant, antimicrobial, and anti-inflammatory properties. In this study, we compared the role of Gossypin with the therapeutically used drug Dexamethasone in the acute lung injury model caused by oleic acid in rats. Thirty rats were divided into five groups; Sham, Oleic acid model, Oleic acid+Dexamethasone (0.1 mg/kg), Oleic acid +Gossypin (10 and 20 mg/kg). Two hours after pretreatment with Dexamethasone or Gossypin, the acute lung injury model was created by injecting 1 g/kg oleic acid into the femoral vein. Three hours following the oleic acid injection, rats were decapitated. Lung tissues were extracted for histological, immunohistochemical, biochemical, PCR, and SEM imaging assessment. The oleic acid injection caused an increase in lipid peroxidation and catalase activity, pathological changes in lung tissue, decreased superoxide dismutase activity, and glutathione level, and increased TNF-alpha, IL-1 ss, IL-6, and IL-8 expression. However, these changes were attenuated after treatment with Gossypin and Dexamethasone. By reducing the expression of proinflammatory cytokines and attenuating oxidative stress, Gossypin pretreatment provides a new target that is equally effective as dexamethasone in the treatment of oleic acid-induced acute lung injury.
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    TRDizin
    Morphological Study of the Intraorbital Muscles (Musculi bulbi) in Rat and Guinea Pig
    (2023) Demirci, B.; Onuk, B.; Tütüncü, Ş.; Gültiken, M.E.
    Intraorbital muscles in the human are similar to those in the mammals as regards morphology and function, except for the absence of the retractor bulbi muscle in the human. The aim of this study is to determine the morphological and morphometric features of the eye muscles in the rat and guinea pig used as a model for various studies. Twen- ty-four bulbus oculi of 12 rats and 32 bulbus oculi of 16 guinea pig were investigated by subgross and histological methods. Certain morphometric data of musculi bulbi were obtained by stereo-microscope. Samples were fixed in 10% formalin for subgross procedures and preserved in Bouin solution for histological methods. The muscular part of the intraorbital muscles in the rat was examined and each muscle was determined to be originated from near the optic foramen and to be attached to sclera close to corneal limbus by tendinous ends. The intraorbital muscles of the guinea pig were relatively weak compared to the body size. The functional part of the intraorbital muscles located around the optic nerve was considerably short and attached to the sclera by the long tendinous ends. Retractor bulbi muscle was small muscle batch located ventrolateral of the optic nerve in rat and substantially weak muscle bundles that envelop the optic nerve in the guinea pig.