Browsing by Author "Cetin F."

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Comparative identification, characterization, and expression analysis of bZIP gene family members in watermelon and melon genomes
(2019-03-02) Unel N.M.; Cetin F.; Karaca Y.; Celik Altunoglu Y.; Baloglu M.C.
The family of basic leucine zipper (bZIP) transcription factors plays diverse crucial roles in numerous biological processes. Despite the identification of bZIP genes in several plants, to our knowledge, bZIP members in watermelon and melon are yet to be comprehensively investigated. The genomes of watermelon and melon encode 59 ClabZIP and 75 CmbZIP putative genes, respectively. Both bZIP protein family members were phylogenetically grouped into seven subfamilies. The majority of bZIP genes in the same subfamily shared similar gene structures and conserved motifs. Chromosome distribution and genetic analysis revealed that 21 duplication events between ClabZIP genes and 106 duplication events between CmbZIP genes have occurred. Further, the three-dimensional structure and functional annotation of bZIP proteins was predicted. For evaluating the expression patterns of ClabZIP and CmbZIP genes, RNA-seq data available in public databases were analyzed. The expression profiles of selected ClabZIP and CmbZIP genes in root and leaf tissues of drought-stressed watermelon and melon were also examined using qRT-PCR. ClabZIP-57, CmbZIP-52, and CmbZIP-31 genes exhibited the highest expression levels after stress exposure in leaf and root tissues. Gene identification studies like the present study offer new perspectives in the analysis of bZIP protein family members and their functions in plants.
Physiological and biochemical responses of wheat species with different ploidy level under drought stress
(2020-12-01) Baloglu M.C.; Cetin F.
Drought is crucial abiotic stress that limits growth and causes a yield reduction in wheat. So, the identification of susceptible and tolerant wheat cultivars to drought is critical for breeders. In this study, physiological and biochemical responses against drought stress were investigated in wheat varieties, Siyez (Triticum monococcum), Kiziltan-91 (Triticum turgidum) and Yüregir-89 (Triticum aestivum) possessing different ploidy levels. Some growth, physiological parameters (membrane permeability, malondialdehyde, H2O2, proline and ascorbic acid) and antioxidant enzyme activity (SOD, CAT, APX and GPX) analyses were performed. The first effect of drought was the increase in root lengths for all species. Ascorbic acid and proline amount raised in roots and shoots at a maximum in Yüregir-89 and at a minimum in Siyez. An increase in the CAT activity was observed in the roots of Yüregir-89 and in the leaves of Kiziltan-91. Similar defensive patterns of SOD and GPX enzymes were monitored in Yüregir-89 and Kiziltan-91 tissues. Genotypic variation arisen from ploidy level contributed by various parameters was also determined by principal component (PCA) analysis under drought stress. As a result of all analyses, Kiziltan-91 and Yüregir-89 wheat varieties could be considered as resistant to drought and Siyez wheat was susceptible to drought. The possible explanations for this might be strong enzymatic and non-enzymatic antioxidant defense mechanisms and high ploidy levels mechanisms in Kiziltan-91 and Yüregir-89 varieties. This study has the potential to become a reference source for studies of the identification of wheat species with a high survival rate under drought in the future.