Browsing by Author "Celik M."

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Comparison of the physicochemical properties of chitosan extracted from shrimp shell waste with different deacetylation degrees
(2017-01-01) Kucukgulmez A.; Eslem Kadak A.; Celik L.; Farivar A.; Celik M.
In this study chitosan with different degrees of deacetylation have been extracted from deep sea pink shrimp (Parapenaeus longirostris) shells not evaluated and discarded as waste products to investigate the effect of deacetylation on physicochemical properties of chitosan. In order to determine physicochemical characterization of the chitosans; moisture and ash contents, degree of deacetylation, molecular weight, apparent viscosity, water and fat binding capacities, Fourier Transform Infrared Spectroscopy, Scanning Electron Microscopy and X-ray diffraction analyses were applied. In addition, the physicochemical characteristics of the chitosan extracted from P. longirostris shells were compared to the commercial chitosan. Low degree deacetylated chitosan exhibited a higher molecular weight, higher viscosity and higher water and fat binding capacities compared to the high degree deacetylated chitosan. Study findings are considered to be useful for the economic use of shrimp wastes in Turkey and light the way for future studies on other crustacean species.
Effects of different degrees of deacetylation and levels of chitosan on performance, egg traits and serum biochemistry of laying hens
(2022-01-01) Farivar A.; Atay A.; Şahan Z.; Serbester U.; Yenilmez F.; Tekeli A.; Küçükgülmez A.; Kadak A.E.; Celik M.; Uzun Y.; Kutlu H.R.; Baykal Çelik L.
The present study was conducted to evaluate whether the deacetylation degree of chitosan (low: 70% vs. high: 90%) and its dietary level (0, 200, 400, 800, 1600 mg/kg diet) would affect laying performance, faeces viscosity, egg quality, egg and serum biochemistry of layers. For the experimental feeding period of 8 weeks, 140 four weeks old Hisex Brown layers were divided into 10 treatment groups, comprising 14 birds each. The birds were housed in individual cages in a complete randomised design. Performance was assessed by recording feed intake, egg weight, daily egg production, egg quality and egg biochemistry. Serum biochemistry parameters were determined at the beginning and end of the experiment and faeces viscosity at the end of the experiment. Feed conversion ratio and faeces viscosity were deteriorated by increased level of chitosan. Lightness of egg yolk was significantly increased in animals receiving high-degree deacetylated chitosan compared to low-degree deacetylated chitosan. Yellowness of egg yolk was affected by interaction of deacetylation degree and level of chitosan. Yolk cholesterol concentration was lower in groups receiving high deacetylated chitosan by increasing chitosan level, while laying hens fed low deacetylated chitosan had a higher level of yolk cholesterol. A significant interaction between degree of deacetylation and chitosan level was determined for serum glucose and calcium concentration. Serum total antioxidant content increased with higher levels of dietary chitosan. In conclusion, dietary level or different degrees of deacetylated chitosan may reduce yolk cholesterol and improve serum antioxidant status. However, feed conversion ratio and faeces viscosity were impaired by increasing levels of chitosan supplementation, and lightness of yolk was increased by supplementation of chitosan with a high degree of deacetylation.
Role of endothelin 1 on proliferation and migration of human MCF-7 cells
(2020-10-01) Cinar I.; Yayla M.; Celik M.; Bilen A.; Bayraktutan Z.
Objective: The aim of this study was to explore the role of endothelin 1 (ET-1) in human breast cancer proliferation and migration and antagonism of endothelin receptor A (ETAR) and endothelin receptor B (ETBR) by using the non-selective dual ETA/ETB receptor antagonist bosentan and determine its anti-proliferative, anti-metastatic, and apoptotic effects demonstrated by nuclear factor kappa B (NF-kB), vascular endothelial growth factor (VEGF), Caspase 3 and Caspase 9 expression on endothelin-induced proliferation of MCF-7 cell line in vitro. Materials and Methods: A total of 8,000 cells were seeded into e-plates 24 hours after the cells were incubated with or without 10-4 M BOS (1 hour before ET-1 treatment); 10-7, 10-8, and 10-9 M ET-1 for 1-4 days. Results: Whether ET-1 is present or not in the tumor area, bosentan exerts anti-proliferative effect on breast cancer. However, ET-1 and bosentan group showed important inhibitory effect on tumor migration compared to bosentan alone, which can be attributed to increased activity of ET-1 axis in the presence of ET-1. The imbalance among the NF-kB, caspases, and VEGF, which are predictive factors of carcinogenesis significantly improved after bosentan administration. Conclusion: Our study definitely demonstrated ET-1 and its critical role in cancer progression with apop-totic and anti-apoptotic pathways (NF-κB) and VEGF expression, and migration analyses were also per-formed. The second major finding was that bosentan inhibited ET-1-mediated effects on tumor proliferation and migration.
The evaluation of shellfish wastes as antimicrobial agent on Rainbow trout
(2018-01-01) Kucukgulmez A.; Kadak A.; Celik M.; Yanar Y.; Gulnaz O.
This study aims to investigate antimicrobial effects of chitosan obtained from shell wastes of Metapenaeus stebbingi on refrigerated rainbow trout fillets. In the study, the fillets were dipped with different concentrations (0.1%, 0.5%, 1%) of extracted and commercial chitosan and were packed with vacuum and air conditions and subsequently, changes in total bacteria were observed during refrigerated storage period (4±1°C). There were decreases in the number of microorganisms of both groups plunged in natural and commercial chitosan during 12 days of storage compared to control group, and the best results was obtained as 0.1% in the groups applied with natural and commercial chitosan (p<0.05). In conclusion, chitosan obtained from shell wastes of M. stebbingi could be used as an effective natural alternative to synthetic antimicrobial agents during the storage period of fish.