Browsing by Author "Celebi, F."

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Effects of iloprost and n-acetylcysteine on ischemia-reperfusion injury and thiol/disulfide hemostasis in rats
(2023.01.01) Yildiz, Y.A.; Altintoprak, F.; Celebi, F.; Oter, V.; Cakar, G.C.; Yazar, H.; Cakiroglu, H.; Muhtaroglu, A.
Aim: Intestinal ischemia occurs after partial or complete obstruction of the intestinal arterial blood flow, and reperfusion injury following the restoration of blood flow. Intestinal ischemia-reperfusion [IIR] damage can cause multiple organ failure and death. In our study, we aimed to observe the effect of ilioprost and N-acetylcysteine on ischemia-reperfusion injury and to show the effect of the Thiol disulfide mechanism in this area. Material and Methods: Thirty Sprague Dawley rats were divided into five groups of six animals each: sham, IIR, IIR+IL, IIR+NAC and IIR+NAC+IL. Intestinal samples and blood were collected after completion of the sham or IIR protocol. Small-bowel samples were evaluated according to the Chiu score. Thiol/disulfide [DS] hemostasis was followed using a novel series of serum biomarkers. Serum concentrations of total thiol, native thiol and disulfide were also determined. Results: The average Chiu score was lower in the IIR + NAC group than in both the IIR and the IIR + IL group, but the differences were not statistically significant. The score in the sham group was significantly lower than those of the other four groups. The level of reduced thiol and the native thiol/total thiol [NT/TT] ratios were higher in groups treated with NAC, IL or both. In the latter groups, oxidized thiol, DS/TT and DS/NT ratios were lower than in the IIR group but the differences between the three treatment groups were not statistically significant. Discussion: The addition of IL to NAC was not more protective than NAC alone in a rat model of IIR injury. Our results suggest that markers of thiol-DS hemostasis can be used as indicators of antioxidant mechanisms in IIR injury.
Molecular insights into the antioxidative and anti-inflammatory effects of P-coumaric acid against bisphenol A-induced testicular injury: In vivo and in silico studies
(2024.01.01) Tekin, S.; Sengul, E.; Yildirim, S.; Aksu, E.H.; Bolat, I.; Çinar, B.; Shadidizaji, A.; Celebi, F.; Warda, M.
This study investigated the protective effects of p-coumaric acid (PCA) against bisphenol A (BPA)-induced testicular toxicity in male rats. The rats were divided into control, BPA, BPA +PCA50, BPA +PCA100, and PCA100 groups. Following a 14-day treatment period, various analyses were conducted on epididymal sperm quality and testicular tissues. PCA exhibited dose-dependent cytoprotective, antioxidant, and anti-inflammatory effects, ameliorating the decline in sperm quality induced by BPA. The treatment elevated antioxidant enzyme activities (SOD, GPx, CAT) and restored redox homeostasis by increasing cellular glutathione (GSH) and reducing malondialdehyde (MDA) levels. PCA also mitigated BPA-induced proinflammatory responses while reinstating anti-inflammatory IL-10 levels. Apoptotic parameters (p53 and p38-MAPK) were normalized by PCA in BPAtreated testicular tissue. Immunohistochemical and immunofluorescent analyses confirmed the cytoprotective and anti-inflammatory effects of PCA, evidenced by the upregulation of HO-1, Bcl-2, and Nrf-2 and the downregulation of the proapoptotic gene Bax in BPA-induced testicular intoxication. PCA corrected the disturbance in male reproductive hormone levels and reinstated testosterone biosynthetic capacity after BPA-induced testicular insult. In silico analyses suggested PCA 's potential modulation of the oxidative stress KEAP1/NRF2/ARE pathway, affirming BPA 's inhibitory impact on P450scc. This study elucidates BPA 's molecular disruption of testosterone biosynthesis and highlights PCA 's therapeutic potential in mitigating BPA 's adverse effects on testicular function, showcasing its cytoprotective, anti-inflammatory, and hormone-regulating properties. The integrated in vivo and in silico approach offers a comprehensive understanding of complex mechanisms, paving the way for future research in reproductive health and toxicology, and underscores the importance of employing BPA-free plastic wares in semen handling.