Browsing by Author "Canli K."

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Antimicrobial activity and chemical composition screening of Anacyclus pyrethrum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient times. The resistance of pathogens to antibiotics became a critical problem, so researches for novel antimicrobial agents are required. Anacyclus pyrethrum (pellitory, Spanish chamomile or Mount Atlas daisy) is commonly used as a traditional medicine, therefore the antimicrobial activity of the root of this medicinal plant was investigated against 17 bacteria and 1 fungi by using disk diffusion method. The test strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy analysis. The results were presented that A. pyrethrum has antimicrobial activity against all tested microbial species except E. faecalis and S. typhimurium. Eighteen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed NMR spectra for 3D structure determination and identification.
Antimicrobial activity and chemical composition screening of Epilobium montanum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient time. The resistance of pathogen to antibiotic is became a critical problem, so novel antimicrobial agent related research is required. Epilobium montanum related antimicrobial research doesn’t exist, therefore root of this medicinal plant investigation was applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial species include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy. The results were presented that E. montanum has antimicrobial activity against all tested microbial species except E. coli. Seventeen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed by NMR spectra for 3D structure determination and identification.
Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme
(2014-01-01) Altuner E.; Canli K.; Akata I.
The anti-infective activities of plant-derived secondary metabolites are under investigation in recent years due to accelerating antibacterial and antifungal resistance rates of microorganisms. In this study, antimicrobial activity of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme were screened against Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria monocytogenes ATCC 7644, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disk diffusion method. It is observed that ethanolic extract of the moss samples have antimicrobial activity against several gram positive and gram negative microorganisms tested. But antimicrobial activity of D. polysetum is notable especially against S. carnosus. These results are the very first data about the antimicrobial activity of C. cuspidata and D. polysetum.
Antimicrobial screening of mnium stellare
(2015-04-11) Canli K.; Altuner E.M.; Akata I.
Many plants contain active substances that are known to be effective in both enhancing the wound healing process and lowering the incidence of wound infections. Previous studies have shown that bryophytes produce a variety of secondary metabolites that present pharmaceutical activities including antimicrobial activity against various pathogenic bacteria and fungi. The aim of this study was to investigate the antimicrobial activity of Mnium stellare against 17 bacterial and 1 fungal strains. Our present study has shown that the ethanol extract of M. stellare has antimicrobial activity against several Gram positive and Gram negative microorganism tested, but its antimicrobial activity is notable especially against B. subtilis, S. typhimirium, S. aureus, S. carnosus, and S. epidermidis. These results are the very first report of the antimicrobial activity of M. stellare.
Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of Allium scorodoprasum subsp. jajlae (Vved.) Stearn
(2023-06-01) Canli K.; Turu D.; Benek A.; Bozyel M.E.; Simsek Ö.; Altuner E.M.
In this study, the chemical composition and biological activity of Allium scorodoprasum subsp. jajlae (Vved.) Stearn were investigated for the first time, focusing on its antimicrobial, antioxidant, and antibiofilm properties. A GC-MS analysis was employed to evaluate the composition of its secondary metabolites, identifying linoleic acid, palmitic acid, and octadecanoic acid 2,3-dihydroxypropyl ester as the major compounds in ethanol extract. The antimicrobial activity of A. scorodoprasum subsp. jajlae was assessed against 26 strains, including standard, food isolate, clinical isolate, and multidrug-resistant ones, as well as three Candida species using the disc diffusion method and the determination of the minimum inhibitory concentration (MIC). The extract showed strong antimicrobial activity against Staphylococcus aureus strains, including methicillin-resistant and multidrug-resistant strains, as well as Candida tropicalis and Candida glabrata. Its antioxidant capacity was evaluated using the DPPH method, revealing a high level of antioxidant activity in the plant. Additionally, the antibiofilm activity of A. scorodoprasum subsp. jajlae was determined, demonstrating a reduction in biofilm formation for the Escherichia coli ATCC 25922 strain and an increase in biofilm formation for the other tested strains. The findings suggest potential applications of A. scorodoprasum subsp. jajlae in the development of novel antimicrobial, antioxidant, and antibiofilm agents.
Biochemical composition and in vitro antimicrobial activity of endemic helichrysum arenarium ssp. aucheriethanol extract
(2021-02-01) Bozyel M.E.; Canli K.; Benek A.; Yetgin A.; Altuner E.M.
The phenomenon of using plants for therapeutic purposes dates back to the earlier times in the history of humanity. Even though there have been remarkable developments in the modern medicine, pharmaceutical, and chemical industries, the therapeutic use of medicinal plants is still up to date. Due to the emergence of antibiotic-resistant strains and the proliferation of side effects in synthetic drugs, the importance of research on the antimicrobial potential and biochemical composition of plant extracts has increased. The main purpose of this present study is to screen both the antibacterial and antifungal activity of endemic Helichrysum arenarium ssp. aucheri ethanol extract by disk diffusion on some Gram-negative and Gram-positive bacteria and Candida albicans, and to identify the major compounds found in the ethanol extract by GC/MS. According to the results obtained from the study, it can be proposed that the extract showed antibacterial and anti-fungal activity for all microorganisms excluding Salmonella typhimurium SL1344, Salmonella infantis, E. coli (food isolate), Escherichia coli ATCC 25922 and Enterobacter aerogenes ATCC 13048, and some chemical compounds were detected, which may be responsible for the antimicrobial activity.
BIOCHEMICAL COMPOSITION, AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SALVIA FRUTICOSA, AN ETHNOMEDICINAL PLANT
(2023-01-01) Bozyel M.E.; Canli K.; Benek A.; Simsek O.; Akata I.; Altuner E.M.
Biochemical, Antioxidant Properties and Antimicrobial Activity of Epiphytic Leafy Liverwort Frullania dilatata (L.) Dumort
(2023-05-01) Simsek O.; Canli K.; Benek A.; Turu D.; Altuner E.M.
In this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort Frullania dilatata (L.) Dumort were investigated. Due to the scarcity and difficulty in obtaining liverworts, research on their bioactivity is limited; thus, this study aimed to uncover the potential of F. dilatata. The antimicrobial activity was evaluated against various microorganisms, including food isolates, clinical isolates, multidrug-resistant strains, and standard strains, using the disk diffusion method and determining the minimum inhibitory concentration (MIC) values. This study represents the first antioxidant investigation on F. dilatata and an antimicrobial study using ethanol extract and the disk diffusion method. Notably, susceptibility was observed in Enterococcus faecalis ATCC 29212, Enterococcus faecium FI, Listeria monocytogenes ATCC 7644, Providencia rustigianii MDR, and Staphylococcus aureus ATCC 25923. The antioxidant capacity was assessed using the DPPH method, emphasizing the high scavenging performance. Gas chromatography-mass spectrometry (GC-MS) analysis identified the primary compounds as frullanolide (19.08%), 2,3-Dimethylanisole (15.21%), linoleic acid (11.11%), palmitic acid (9.83%), and valerenic acid (5.3%). The results demonstrated the significant antimicrobial activity of F. dilatata against the tested microorganisms and its potent antioxidant properties. These findings emphasize the potential of F. dilatata as a promising source of natural antimicrobial and antioxidant agents, underscoring the importance of further investigation into its bioactive compounds and elucidating the mechanisms of action in future studies.
Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
(2023-08-01) Canli K.; Bozyel M.E.; Turu D.; Benek A.; Simsek O.; Altuner E.M.
Biological Activities and Biochemical Composition of Endemic Achillea fraasii
(2023-04-01) Tunca-Pinarli Y.; Benek A.; Turu D.; Bozyel M.E.; Canli K.; Altuner E.M.
In this study, we investigated the antimicrobial, antioxidant, and antibiofilm activities and the biochemical composition of Achillea fraasii. The antimicrobial activity of A. fraasii ethanol extract (AFEt) was tested against 48 strains, and this is the first study testing the antimicrobial activity of this plant to this extent. The antioxidant activity was determined using the DPPH assay, and the antibiofilm activity of A. fraasii aqueous extract (AFAq) against five strains was assessed. The chemical composition of the plant extract was determined using GC-MS with artemisia ketone (19.41%) as the main component. The findings indicated that AFEt displayed antimicrobial activity against 38 strains, with a particular efficacy observed against various Staphylococcus aureus strains, such as S. aureus ATCC 25923, clinically isolated, multidrug resistant (MDR), and methicillin-resistant (MRSA) strains. In addition, the highest activity was observed against Enterococcus faecium. Moreover, the extract demonstrated activity against Candida strains. The plant extract also showed relatively good antioxidant activity compared to ascorbic acid, with an EC50 value of 55.52 µg/mL. However, AFAq acted as a biofilm activator against Escherichia coli ATCC 25922, increasing the biofilm formation by 2.63-fold. In conclusion, our study demonstrates the potential of A. fraasii as a source of antimicrobial and antioxidant agents.
Chemical composition and in vitro antimicrobial activity of matthiola tricuspidata ethanol extract
(2021-10-01) Canli K.; Bozvel M.E.; Benek A.; Yetgin A.; Senturan M.; Altuner E.M.
It is widely acccptcd by scientists that innovation of new antimicrobial agents has extreme im-portance for the sake of the tiiturc of human beings; therefore the researches about the antimicrobial potentials of plant extracts and their biochemical composition have great importance. This present study aims to investigate the antimicrobial activity of Mat-thiola tricuspidata ethanol extract against several Gram-positive and Gram-negative microorganisms and Candida albicans by disk diffusion method, and to identity its chemical composition by GasChroma-tography-Mass Spectrometry. As a result, it was observed that the cxtract showed activity for all microorganisms excluding Enterobacter aerogenes ATCC 13048, Escherichia coli ATCC 25922, E. coli, Klebsiella pneumoniae, Salmonella infantis, and Salmonella typhimurium SL1344 and some chcmical compounds were dctcctcd, which may be responsible for the antimicrobial activity.
Comparison of Antimicrobial Activity of Allium sativum Cloves from China and Taşköprü, Turkey
(2018-01-01) Yetgin A.; Canli K.; Altuner E.
In this study, antimicrobial activities of two different samples of Allium sativum L. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species of Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, and Staphylococcus were used for testing antibacterial activity. In addition, antifungal activity against Candida albicans was also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.
Determination of the chemical composition and antimicrobial activity of Frankenia hirsuta
(2017-12-24) Canli K.; Şimşek Ö.; Yetgin A.; Altuner E.
Frankenia hirsuta is widely located in Turkey, but the antimicrobial potential and biochemical composition analysis of it weren’t determined yet. By using the disk diffusion method, the susceptibility of 17 bacteria and 1 fungi were analyzed, which included Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera. 0.8, 1.5 and 3.1 mg of samples were prepared by using absolute ethanol. The bioactive composition of the plant extract was determined by gas chromatography- mass spectroscopy, and National Institute of Standards and Technology library was used for the mass spectra analysis. The results showed that F. hirsuta had antimicrobial activity against all of the studied micro-organisms except E. aerogenes and E. coli. Several active metabolites were identified, but some composition of this sample didn’t match with the library. These results are the first report for the antimicrobial potential and biochemical composition of F. hirsuta.
In Vitro Antimicrobial Activity Screening of Ethanol Extract of Lavandula stoechas and Investigation of Its Biochemical Composition
(2019-01-01) Canli K.; Yetgin A.; Benek A.; Bozyel M.; Altuner E.
The aim of this study was to test antimicrobial activity of ethanol extract of Lavandula stoechas against 22 bacteria and 1 yeast. Also, biochemical composition of the extract was investigated. A wide range of Gram-positive, Gram-negative microorganisms, and multidrug resistant bacteria were selected to test the antimicrobial activity. As a result, the extract is observed to contain fenchone (bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl-, (1R)-) and camphor (+)-2-bornanone) as major components and showed antimicrobial activity against all studied microorganisms except Escherichia coli ATCC 25922 and Klebsiella pneumoniae. The results of the study present that L. stoechas is active against MDR strains too.
In Vitro antimicrobial activity screening of xylaria hypoxylon
(2016-01-01) Canli K.; Akata I.; Altuner E.M.
Background: Fungi have a potential of using both as nutritive and medicinal food stuff. Because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. The aim of this study is to determine the in vitro antimicrobial activity of Xylaria hypoxylon, which were collected from Yomra, Trabzon, Turkey. Materials and Methods: X. hypoxylon samples were air dried and extracted by using ethanol. Antimicrobial activity of X. hypoxylon ethanol extracts were investigated against 21 bacterial and 2 fungal strains, namely, Bacillus subtilis DSMZ 1971, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disk diffusion method. Results: It is observed that ethanol extracts of X. hypoxylon has antimicrobial activity against several Gram positive and Gram negative microorganisms tested. As a result of the study, an antimicrobial activity of X. hypoxylon found against most of strains used in the study. Conclusion: The results of our study clearly puts forward that X. hypoxylon could have a possible medicinal use.
In vitro antimicrobial screening of cerena unicolor (Bull.) murrill (Polyporaceae fr. ex Corda)
(2012-12-01) Altuner E.; Akata I.; Canli K.
The aim of this study is to determine the antimicrobial activity of Cerena unicolor ethanolic extract against 19 bacterial and 1 fungal strains. C. unicolor samples which were collected from Yomra, Trabzon, TURKEY were extracted with ethanol. Extracts were investigated for in vitro antimicrobial activity against a wide range of strains including Bacillus cereus DSMZ 31, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durons, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumonia, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa ATCC 27853, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disc diffusion method. It is observed that ethanolic extract of C. unicolor has antimicrobial activity against several gram positive microorganims tested. As a result of the study, it could be concluded that ethanolic extract of C. unicolor is active against several microorganisms but its antimicrobial activity is notable especially against B. cereus DSMZ 31, S. carnosus MC1.B and S. epidermidis DSMZ 20044. © by PSP.
In vitro antimicrobial screening of Hedwigia ciliata Var. leucophaea and determination of the ethanol extract composition by gas chromatography/mass spectrometry (GC/MS)
(2014-08-01) Canli K.; Cetin B.; Altuner E.M.; Türkmen Y.; Uzek U.; Dursun H.
Many plants contain active substances that are known to be effective in both enhancing the wound healing process and lowering the incidence of wound infections. Previous studies have shown that bryophytes produce a variety of secondary metabolites that present pharmaceutical activities including antimicrobial activity against various pathogenic bacteria and fungi. The aim of this study was to investigate the antimicrobial activity of Hedwigia ciliata var. leucophaea (He) against 17 bacterial and 1 fungal strains and analyse the composition by GC/MS. He samples were collected from Akdag Mountain (Amasya, TURKEY) and subjected to ethanol extraction after air drying. The in vitro antimicrobial activities of He extracts having 9 mg.mL-1 concentrations were assessed against a wide range of strains by disk diffusion method and the results were supported by a MIC (minimum inhibitory concentration) test. Our present study has shown that the ethanol extract of He has antimicrobial activity against several Gram positive and Gram negative microorganisms tested, but its antimicrobial activity is notable especially against B. subtilis, E. faecalis, S. camosus, and S. epidermidis. These results are the very first report of the antimicrobial activity of He and its.composition. The results obtained herein indicate that ethanol extract of He contains several active metabolites and it is active against several microorganisms. But further researches, especially cytotoxicity and genotoxicity tests are needed to be conducted to conclude whether He extracts can be used safely in terms of their antimicrobial activity.
In vitro antimicrobial screening of Lycoperdon lividium and determination of the ethanol extract composition by gas chromatography/mass spectrometry
(2016-01-01) Canli K.; Altuner E.; Akata I.; Turkmen Y.; Uzek U.
The aim of this study was to investigate the antimicrobial activity of Lycoperdon lividium against 17 bacterial and 1 fungal strains and analyse the composition of ethanol extracts by GC/MS. The in vitro antimicrobial activity of L. lividium extracts having 15 mg/mL concentration was assessed against a wide range of strains by disk diffusion method. The ethanol extract of L. lividium had antimicrobial activity against several microorganism tested, but it was active especially against S. carnosus. The results obtained herein indicate that L. lividium contains several active metabolites.