Browsing by Author "Bolat, I."

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    Web of Science
    Investigation of the subchronic effects of low-dose pesticide mixture on rat testes
    (2023.01.01) Ömür, A.D.; Sevim, C.; Saglam, Y.S.; Bolat, I.; Genç, S.; Yeni, Y.; Aydin, M.A.; Sunar, S.; Koçak, G.; Akarsu, S.A.
    Pesticides are chemical agents used against living things such as insects, rodents, and weeds that cause toxicity in various tissues, including reproductive organs. In order to increase the effectiveness of pesticides, active ingredients are mixed with various formulations. The current study tried to determine the effects of pesticides and their mixtures on testicular tissue in rats. For this purpose, chlormequat chloride (CCC), pirimiphos methyl (PMM), glyphosate ( GLY), tebuconazole (TBZ), chlorpyrifos methyl (CPM), deltamethrin (DLM), and their mix (acceptable daily intake (ADI) and ADIx10) were administried to Sprague-Dawley rats for 90 days. As a result of the examinations, the ADIx10 group showed the most damaging effect by showing a statistical difference in terms of sperm motility and membrane integrity. The GLY group had a similar effect on membrane integrity as the ADIx10 group, while it had a moderately detrimental effect on motility. Degenerations of spermatocytes, necrosis, and edema in intertubular spaces were observed in all pesticide groups. Similarly, 8-hydroxyguanosine (8-OHdG) expression and Caspase-3 expression were moderate in all pesticide groups. Moreover, 8-OHdG expression and Caspase-3 expression were higher in the ADI and ADIx10 groups compared to other groups. As a result, pesticides and their mixtures cause histopathological changes in the testicular tissue of rats and decrease sperm quality.
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    Web of Science
    Molecular insights into the antioxidative and anti-inflammatory effects of P-coumaric acid against bisphenol A-induced testicular injury: In vivo and in silico studies
    (2024.01.01) Tekin, S.; Sengul, E.; Yildirim, S.; Aksu, E.H.; Bolat, I.; Çinar, B.; Shadidizaji, A.; Celebi, F.; Warda, M.
    This study investigated the protective effects of p-coumaric acid (PCA) against bisphenol A (BPA)-induced testicular toxicity in male rats. The rats were divided into control, BPA, BPA +PCA50, BPA +PCA100, and PCA100 groups. Following a 14-day treatment period, various analyses were conducted on epididymal sperm quality and testicular tissues. PCA exhibited dose-dependent cytoprotective, antioxidant, and anti-inflammatory effects, ameliorating the decline in sperm quality induced by BPA. The treatment elevated antioxidant enzyme activities (SOD, GPx, CAT) and restored redox homeostasis by increasing cellular glutathione (GSH) and reducing malondialdehyde (MDA) levels. PCA also mitigated BPA-induced proinflammatory responses while reinstating anti-inflammatory IL-10 levels. Apoptotic parameters (p53 and p38-MAPK) were normalized by PCA in BPAtreated testicular tissue. Immunohistochemical and immunofluorescent analyses confirmed the cytoprotective and anti-inflammatory effects of PCA, evidenced by the upregulation of HO-1, Bcl-2, and Nrf-2 and the downregulation of the proapoptotic gene Bax in BPA-induced testicular intoxication. PCA corrected the disturbance in male reproductive hormone levels and reinstated testosterone biosynthetic capacity after BPA-induced testicular insult. In silico analyses suggested PCA 's potential modulation of the oxidative stress KEAP1/NRF2/ARE pathway, affirming BPA 's inhibitory impact on P450scc. This study elucidates BPA 's molecular disruption of testosterone biosynthesis and highlights PCA 's therapeutic potential in mitigating BPA 's adverse effects on testicular function, showcasing its cytoprotective, anti-inflammatory, and hormone-regulating properties. The integrated in vivo and in silico approach offers a comprehensive understanding of complex mechanisms, paving the way for future research in reproductive health and toxicology, and underscores the importance of employing BPA-free plastic wares in semen handling.