Browsing by Author "Bilen A."

Now showing 1 - 5 of 5

Does daily fasting shielding kidney on hyperglycemia-related inflammatory cytokine via TNF-α, NLRP3, TGF-β1 and VCAM-1 mRNA expression
(2021-11-01) Bilen A.; Calik I.; Yayla M.; Dincer B.; Tavaci T.; Cinar I.; Bilen H.; Cadirci E.; Halici Z.; Mercantepe F.
This study aimed to investigate the effects of blood glucose control and the kidneys' functions, depending on fasting, in the streptozotocin-induced diabetes model in rats via TNF-α, NLRP-3, TGF-β1 and VCAM-1 mRNA expression in the present study. 32 Wistar albino rats were allocated randomly into four main groups; H (Healthy, n = 6), HF (Healthy fasting, n = 6), D (Diabetes, n = 10), DF (Diabetes and fasting, n = 10). Blood glucose and HbA1c levels significantly increased in the D group compared to the healthy ones (p < 0.05). However, the fasting period significantly improved blood glucose and HbA1c levels 14 days after STZ induced diabetes in rats compared to the D group. Similar findings we obtained for serum (BUN-creatinine) and urine samples (creatinine and urea levels). STZ induced high glucose levels significantly up-regulated TNF-α, NLRP-3, TGF-β1 and VCAM-1 mRNA expression and fasting significantly decreased these parameters when compared to diabetic rats. Histopathological staining also demonstrated the protective effects of fasting on diabetic kidney tissue. In conclusion, intermittent fasting regulated blood glucose level as well as decreasing harmful effects of diabetes on kidney tissue. The fasting period significantly decreased the hyperglycemia-related inflammatory cytokine damage on kidneys and also reduced apoptosis in favor of living organisms.
Influence of tetra (Cotinus coggygria) extract against Vibrio anguillarum infection in koi carp, Cyprinus carpio with reference to haematological and immunological changes
(2013-12-09) Bilen S.; Yilmaz S.; Bilen A.
In this study, the effect of the methanolic extract of tetra (Cotinus coggygria) against Vibrio anguillarum in cultured koi carp (Cyprinus carpio carpio) was investigated. Three different concentrations of tetra extract (0, 0.5, 1, 1.5 g kg-1 of feed) were individually mixed with the basal diet and fed to koi (average body weight of 4,14±0,08 g) for a 4-week period to assess the immunomodulatory characteristics of tetra. At the end of the study, koi was challenged with a strain of Vibrio anguillarum (108 CFU ml-1). Growth performance was not affected by dietary tetra extract intake (P>0.05). Nitroblue tetrazolium (NBT) activity was found to be the highest in the 1.5 g kg-1 containing group. Lysozyme and myeloperoxidase activity of the experimental groups was significantly improved in the tetra groups compared to the control group (P<0.05). Red blood cell and mean corpuscular haemoglobin concentration (MCHC) were higher in 1 g kg-1 tetra containing group (P<0.05). This study shows that the koi treated with 0, 0.5, 1 and 1.5 g kg-1 tetra extract prior the challenge with live V. anguillarum had 37.50%, 31.94%, 18.06% and 12.50% mortality. According to study results, tetra is an effective non-specific immunostimulant for koi. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey.
Role of endothelin 1 on proliferation and migration of human MCF-7 cells
(2020-10-01) Cinar I.; Yayla M.; Celik M.; Bilen A.; Bayraktutan Z.
Objective: The aim of this study was to explore the role of endothelin 1 (ET-1) in human breast cancer proliferation and migration and antagonism of endothelin receptor A (ETAR) and endothelin receptor B (ETBR) by using the non-selective dual ETA/ETB receptor antagonist bosentan and determine its anti-proliferative, anti-metastatic, and apoptotic effects demonstrated by nuclear factor kappa B (NF-kB), vascular endothelial growth factor (VEGF), Caspase 3 and Caspase 9 expression on endothelin-induced proliferation of MCF-7 cell line in vitro. Materials and Methods: A total of 8,000 cells were seeded into e-plates 24 hours after the cells were incubated with or without 10-4 M BOS (1 hour before ET-1 treatment); 10-7, 10-8, and 10-9 M ET-1 for 1-4 days. Results: Whether ET-1 is present or not in the tumor area, bosentan exerts anti-proliferative effect on breast cancer. However, ET-1 and bosentan group showed important inhibitory effect on tumor migration compared to bosentan alone, which can be attributed to increased activity of ET-1 axis in the presence of ET-1. The imbalance among the NF-kB, caspases, and VEGF, which are predictive factors of carcinogenesis significantly improved after bosentan administration. Conclusion: Our study definitely demonstrated ET-1 and its critical role in cancer progression with apop-totic and anti-apoptotic pathways (NF-κB) and VEGF expression, and migration analyses were also per-formed. The second major finding was that bosentan inhibited ET-1-mediated effects on tumor proliferation and migration.
Systemic immune inflammation index in differentiated thyroid cancers
(2022-04-01) Kars A.; Sahin A.; Kılıc K.; Sakat M.S.; Bilen A.
Objective. The aim of this study was to investigate the relationship between differentiated thyroid carcinomas (DTCs), histopathological findings and systemic immune-inflammation index (SII) [neutrophil (N) x platelet (P) / lymphocyte (L)] values. Methods. 93 patients with DTC were included. N, P and L levels were measured, and the relationship between the SII and histopathological findings was determined. The results were compared with the values of 33 healthy controls. Results. SII values were significantly higher in the patient group than in the control group (p = 0.000). Tumour pathology diagnosis had no significant effect on SII (p = 0.90). Perineural lymphovascular and capsule invasion and extrathyroidal extension also had no significant effect on SII values. SII was significantly higher in patients with more than one tumour focus (p = 0.01). No significant relationship was determined between tumour diameter and SII. Conclusions. SII is higher in patients with DTC compared to the healthy population. High SII values may be associated with multifocality. According to the results of this study, SII does not affect the histological type, perineural, lymphovascular and capsule invasion, or extrathyroidal extension of DTC.
The effects of oxygen supplementation on growth and survival of rainbow trout (Oncorhynchus mykiss) in different stocking densities
(2015-01-01) Bilen S.; Bilen A.; Önal U.
In this study, the effects of oxygen supplementation in different stocking densities of rainbow trout (Oncorhynchus mykiss) on the specific growth rates (SGR), feed conversion ratios (FCR) and survival rates were investigated in a commercial-scale culture system. High density cultures were maintained in a total of nine (12 m³) flow-through systems supplemented with O2. Initial stocking rates for 3 different treatments with three replicates were 26.88±0.30 (low density), 36.72±0.26 (medium density) and 55.03±0.14kg m^-3 (high density), corresponding to a total of 5800, 8000 and 12000 fish/tank, respectively. Water flow rate for each tank was maintained at 11±1.1 L sec^-1 (660 L min^-1) over the study period for each raceway, and the temperature was 12.3±0.8°C throughout the experimental period. At the end of the study, there were no significant differences in the survival rates and SGR among treatments (p>0.05). However, FCR was negatively affected in the high density treatment (p<0.05). Overall, our results indicated that oxygen supplementation supported fish growth and survival in high stocking densities. However, high production cost is prohibitive and further studies are required to reduce costs.