Browsing by Author "Bhat R.A."

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Correction to: Effects of heavy pollution in different water bodies on male rainbow trout (Oncorhynchus mykiss) reproductive health (Environmental Science and Pollution Research, (2022), 30, 9, (23467-23479), 10.1007/s11356-022-23670-w)
(2023-02-01) Bhat R.A.; Saoca C.; Cravana C.; Fazio F.; Guerrera M.C.; Labh S.N.; Kesbiç O.S.
The images of Figs. 3 and 4 should be interchanged. The Original article has been corrected.
Effects of heavy pollution in different water bodies on male rainbow trout (Oncorhynchus mykiss) reproductive health
(2023-02-01) Bhat R.A.; Saoca C.; Cravana C.; Fazio F.; Guerrera M.C.; Labh S.N.; Kesbiç O.S.
This research aimed to investigate the influence of heavy metals on the reproductive health of rainbow trout (Oncorhynchus mykiss) captured from two locations, Verinag (S1) and Panzath (S2). Sixty (n. 60) mature rainbow trout samples (30 from each site) with body weights ranging from 400 to 650 g were collected from Verinag hatchery, which was less polluted (S1), and Panzath hatchery, which was more polluted (S2). The findings revealed significant differences between the two sites, S1 (less polluted) and S2 (more polluted), as well as significant variations within the tissues. Iron (Fe) and Zinc (Zn) were found to be highest in sampling water as well as in fish tissues sampled from S2 sites. Similarly, concerning S1 captured fishes, histopathological examination of testes from S2 captured fish was found. Testicular abnormalities that included disorganization of the seminiferous tubules, reduction in the number of germ cells (sperm cells, spermatozoa), vacuolization, and large empty areas in the seminiferous epithelium were found. In testicular cells, the frequency of apoptotic cells collected from S2 water increased significantly (P < 0.05). SOD, catalase, and glutathione peroxidase activity increased in S1 captured fishes but decreased in S2 captured fishes. MDA levels gradually increased in S2 captured fish, and the degree of heavy metal stress was positively correlated (P < 0.05). In male rainbow trout, testosterone and 11-ketotestosterone levels increased substantially in S1 captured fishes. While the fatty acids of testes in S2 fishes decreased with respect to S1 fishes. In conclusion, S2 captured fish suffered more damage due to heavy metals including cellular damage, apoptosis, oxidative damage, and altered steroid hormones when compared to fish from S1 waters.
Lipid and Protein Content in Rainbow Trout in Relation to Gonadal Growth
(2022-12-01) Bhat R.A.; Saoca C.; Arfuso F.; Pino C.; D'Alessandro E.; Zumbo A.; Fazio F.; Kesbiç O.S.
The purpose of this study was to examine various lipid classes as well as the protein content in rainbow trout ovaries. A total of 80 samples of Oncorhynchus mykiss (Walbaum 1792) were collected from Kokernag and Verinag hatcheries (Jammu and Kashmir, India) between 2017 and 2019. The mature stage in female fish had the highest gonadosomatic index (GSI) and minimal GSI in the spent stage. Higher lipid content of the ovary was reported during the mature stage (36%), as compared to other pre-breeding stages. Similarly, lipid classes also showed fluctuation during the development of the ovary. The lipid classes, i.e. glycolipids, free fatty acids, cholesterol and triglycerides, had a higher content in the mature stage, while the phospholipid content was higher in the mature stage. The protein content was also higher in the mature ovary than in the other developmental stages. The study provides reference values for various biochemical parameters in rainbow trout that could be useful for population monitoring programs and for the development of diets and management methods for fish production under controlled conditions.
Toxic effect of heavy metals on ovarian deformities, apoptotic changes, oxidative stress, and steroid hormones in rainbow trout
(2023-01-01) Bhat R.A.; Bakhshalizadeh S.; Guerrera M.C.; Kesbiç O.S.; Fazio F.
Background: As is well known, the pollution in the aquatic environment in which fish grow has a direct impact on aquaculture practices. Pollution in aquatic systems because of multiple adverse effects on fish metabolic processes, especially the reproductive systems. Aim: The goal of this study was to assess the severity of pollution impact in two different hatcheries, Verinag hatchery, Site 1 (S1) and Panzath hatchery, Site 2 (S2) in Anantnag region, using histopathological, ultrastructural, oxidative stress, genotoxic, and hormonal analysis in rainbow trout gonad (ovary). M&M: Fish were collected between May 2018 and April 2019 from two locations, Verinag hatchery (S1) and Panzath hatchery (S2), which were affected by heavy metals. Results: The histological and ultrastructural examination of rainbow trout ovaries from the Verinag hatchery (S1) revealed normal structure in growing oocytes in rainbow trout at various stages based on morphological features while the fish ovaries in the Panzath hatchery (S2) showed various deformities and irregularly shaped oocytes. The surfaces of some of these oocytes were wrinkled, rough, or distorted. Apoptotic studies revealed that the frequency of apoptotic cells collected from S2 water was significantly increased in ovarian cells (P < 0.05). The activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were found to be increased in fish collected from S1 but decreased in fish collected from S2. In S2 caught fish, malondialdehyde (MDA) levels were found to increase gradually, and the degree of heavy metal stress was positively correlated (p < 0.05). The comet assay was used to determine the induction of DNA damage in ovarian cells. The induction of DNA damage was found to be significantly higher (p < 0.05) in S2 fish specimens compared to fish from S1. On comparing the DNA damage of the rainbow trout from the two sampling sites, it was revealed that the fish is much more sensitive to aquatic contaminants. Regarding steroid hormones, higher levels of progesterone and estrogen were reported in the fish samples collected from S1 as compared to S2 captured fish. Conclusion: In conclusion, the comparative study of fish from two different sites viz. Verinag hatchery (S1) and Panzath hatchery (S2) revealed that S2 sampled fish suffered more heavy metal damage, including cellular deformities, apoptosis, oxidative damage, and altered steroid hormones.