Browsing by Author "Benek A."

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Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of Allium scorodoprasum subsp. jajlae (Vved.) Stearn
(2023-06-01) Canli K.; Turu D.; Benek A.; Bozyel M.E.; Simsek Ö.; Altuner E.M.
In this study, the chemical composition and biological activity of Allium scorodoprasum subsp. jajlae (Vved.) Stearn were investigated for the first time, focusing on its antimicrobial, antioxidant, and antibiofilm properties. A GC-MS analysis was employed to evaluate the composition of its secondary metabolites, identifying linoleic acid, palmitic acid, and octadecanoic acid 2,3-dihydroxypropyl ester as the major compounds in ethanol extract. The antimicrobial activity of A. scorodoprasum subsp. jajlae was assessed against 26 strains, including standard, food isolate, clinical isolate, and multidrug-resistant ones, as well as three Candida species using the disc diffusion method and the determination of the minimum inhibitory concentration (MIC). The extract showed strong antimicrobial activity against Staphylococcus aureus strains, including methicillin-resistant and multidrug-resistant strains, as well as Candida tropicalis and Candida glabrata. Its antioxidant capacity was evaluated using the DPPH method, revealing a high level of antioxidant activity in the plant. Additionally, the antibiofilm activity of A. scorodoprasum subsp. jajlae was determined, demonstrating a reduction in biofilm formation for the Escherichia coli ATCC 25922 strain and an increase in biofilm formation for the other tested strains. The findings suggest potential applications of A. scorodoprasum subsp. jajlae in the development of novel antimicrobial, antioxidant, and antibiofilm agents.
Biochemical composition and in vitro antimicrobial activity of endemic helichrysum arenarium ssp. aucheriethanol extract
(2021-02-01) Bozyel M.E.; Canli K.; Benek A.; Yetgin A.; Altuner E.M.
The phenomenon of using plants for therapeutic purposes dates back to the earlier times in the history of humanity. Even though there have been remarkable developments in the modern medicine, pharmaceutical, and chemical industries, the therapeutic use of medicinal plants is still up to date. Due to the emergence of antibiotic-resistant strains and the proliferation of side effects in synthetic drugs, the importance of research on the antimicrobial potential and biochemical composition of plant extracts has increased. The main purpose of this present study is to screen both the antibacterial and antifungal activity of endemic Helichrysum arenarium ssp. aucheri ethanol extract by disk diffusion on some Gram-negative and Gram-positive bacteria and Candida albicans, and to identify the major compounds found in the ethanol extract by GC/MS. According to the results obtained from the study, it can be proposed that the extract showed antibacterial and anti-fungal activity for all microorganisms excluding Salmonella typhimurium SL1344, Salmonella infantis, E. coli (food isolate), Escherichia coli ATCC 25922 and Enterobacter aerogenes ATCC 13048, and some chemical compounds were detected, which may be responsible for the antimicrobial activity.
BIOCHEMICAL COMPOSITION, AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SALVIA FRUTICOSA, AN ETHNOMEDICINAL PLANT
(2023-01-01) Bozyel M.E.; Canli K.; Benek A.; Simsek O.; Akata I.; Altuner E.M.
Biochemical, Antioxidant Properties and Antimicrobial Activity of Epiphytic Leafy Liverwort Frullania dilatata (L.) Dumort
(2023-05-01) Simsek O.; Canli K.; Benek A.; Turu D.; Altuner E.M.
In this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort Frullania dilatata (L.) Dumort were investigated. Due to the scarcity and difficulty in obtaining liverworts, research on their bioactivity is limited; thus, this study aimed to uncover the potential of F. dilatata. The antimicrobial activity was evaluated against various microorganisms, including food isolates, clinical isolates, multidrug-resistant strains, and standard strains, using the disk diffusion method and determining the minimum inhibitory concentration (MIC) values. This study represents the first antioxidant investigation on F. dilatata and an antimicrobial study using ethanol extract and the disk diffusion method. Notably, susceptibility was observed in Enterococcus faecalis ATCC 29212, Enterococcus faecium FI, Listeria monocytogenes ATCC 7644, Providencia rustigianii MDR, and Staphylococcus aureus ATCC 25923. The antioxidant capacity was assessed using the DPPH method, emphasizing the high scavenging performance. Gas chromatography-mass spectrometry (GC-MS) analysis identified the primary compounds as frullanolide (19.08%), 2,3-Dimethylanisole (15.21%), linoleic acid (11.11%), palmitic acid (9.83%), and valerenic acid (5.3%). The results demonstrated the significant antimicrobial activity of F. dilatata against the tested microorganisms and its potent antioxidant properties. These findings emphasize the potential of F. dilatata as a promising source of natural antimicrobial and antioxidant agents, underscoring the importance of further investigation into its bioactive compounds and elucidating the mechanisms of action in future studies.
Biochemical, Antioxidant Properties and Antimicrobial Activity of Steno-Endemic Origanum onites
(2023-08-01) Canli K.; Bozyel M.E.; Turu D.; Benek A.; Simsek O.; Altuner E.M.
Biological Activities and Biochemical Composition of Endemic Achillea fraasii
(2023-04-01) Tunca-Pinarli Y.; Benek A.; Turu D.; Bozyel M.E.; Canli K.; Altuner E.M.
In this study, we investigated the antimicrobial, antioxidant, and antibiofilm activities and the biochemical composition of Achillea fraasii. The antimicrobial activity of A. fraasii ethanol extract (AFEt) was tested against 48 strains, and this is the first study testing the antimicrobial activity of this plant to this extent. The antioxidant activity was determined using the DPPH assay, and the antibiofilm activity of A. fraasii aqueous extract (AFAq) against five strains was assessed. The chemical composition of the plant extract was determined using GC-MS with artemisia ketone (19.41%) as the main component. The findings indicated that AFEt displayed antimicrobial activity against 38 strains, with a particular efficacy observed against various Staphylococcus aureus strains, such as S. aureus ATCC 25923, clinically isolated, multidrug resistant (MDR), and methicillin-resistant (MRSA) strains. In addition, the highest activity was observed against Enterococcus faecium. Moreover, the extract demonstrated activity against Candida strains. The plant extract also showed relatively good antioxidant activity compared to ascorbic acid, with an EC50 value of 55.52 µg/mL. However, AFAq acted as a biofilm activator against Escherichia coli ATCC 25922, increasing the biofilm formation by 2.63-fold. In conclusion, our study demonstrates the potential of A. fraasii as a source of antimicrobial and antioxidant agents.
Chemical composition and in vitro antimicrobial activity of matthiola tricuspidata ethanol extract
(2021-10-01) Canli K.; Bozvel M.E.; Benek A.; Yetgin A.; Senturan M.; Altuner E.M.
It is widely acccptcd by scientists that innovation of new antimicrobial agents has extreme im-portance for the sake of the tiiturc of human beings; therefore the researches about the antimicrobial potentials of plant extracts and their biochemical composition have great importance. This present study aims to investigate the antimicrobial activity of Mat-thiola tricuspidata ethanol extract against several Gram-positive and Gram-negative microorganisms and Candida albicans by disk diffusion method, and to identity its chemical composition by GasChroma-tography-Mass Spectrometry. As a result, it was observed that the cxtract showed activity for all microorganisms excluding Enterobacter aerogenes ATCC 13048, Escherichia coli ATCC 25922, E. coli, Klebsiella pneumoniae, Salmonella infantis, and Salmonella typhimurium SL1344 and some chcmical compounds were dctcctcd, which may be responsible for the antimicrobial activity.
In Vitro Antimicrobial Activity Screening of Ethanol Extract of Lavandula stoechas and Investigation of Its Biochemical Composition
(2019-01-01) Canli K.; Yetgin A.; Benek A.; Bozyel M.; Altuner E.
The aim of this study was to test antimicrobial activity of ethanol extract of Lavandula stoechas against 22 bacteria and 1 yeast. Also, biochemical composition of the extract was investigated. A wide range of Gram-positive, Gram-negative microorganisms, and multidrug resistant bacteria were selected to test the antimicrobial activity. As a result, the extract is observed to contain fenchone (bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl-, (1R)-) and camphor (+)-2-bornanone) as major components and showed antimicrobial activity against all studied microorganisms except Escherichia coli ATCC 25922 and Klebsiella pneumoniae. The results of the study present that L. stoechas is active against MDR strains too.