Browsing by Author "Aygün A."

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Biological synthesis of silver nanoparticles using Rheum ribes and evaluation of their anticarcinogenic and antimicrobial potential: A novel approach in phytonanotechnology
(2020-02-05) Aygün A.; Gülbağça F.; Nas M.S.; Alma M.H.; Çalımlı M.H.; Ustaoglu B.; Altunoglu Y.C.; Baloğlu M.C.; Cellat K.; Şen F.
This paper reports the anticarcinogenic and antimicrobial properties of silver nanoparticles (Ag NPs) obtained by green synthesis using the extract of Rheum ribes (R. ribes), a medicinal plant. For the synthesis of Ag NPs, the ethanolic extracts of R. ribes were used as a reducing as well as the stabilizing agent. For the characterization of Ag NPs, advanced analytical methods such as transmission electron microscopy (TEM), X-Ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and UV–vis spectrophotometry were performed. The synthesized Ag NPs obtained from R. ribes were evaluated as a cytotoxic agent against MDA-MB-231 breast carcinoma cell line. The IC50 values of the nanoparticles were ranged from 165 to 99 μg/mL against MDA-MB 231 cell line for 24 h and 48 h, respectively. The results show that the use of Ag NPs at low concentrations show the toxic effect in the cancer cells. In addition, the results of experiments on gram-positive (Staphylococcus aureus (S. aureus), Methicillin-resistant Staphylococcus aureus (MRSA) and Bacillus subtilis (B. subtilis)) and gram-negative (Escherichia coli (E. coli)) bacteria showed that the Ag NPs had high antimicrobial activity. The results suggest that Ag NPs can be developed as potential anticancer and antibacterial agents.
Evaluation of biological activity of 5-fluoro-isatin thiosemicarbazone derivatives
(2020-06-01) Ramadan M.A.G.; Baloglu M.C.; Altunoglu Y.C.; Kandemirli F.; Burhan H.; Aygün A.; Sayiner H.S.; Ozyigit F.; Şen F.
Isatin based materials can exhibit a wide range of biological activities including antimicrobial, antiviral, antifungal, anthelmintic, antitumor, anti-HIV, anti-inflammatory, antidepressant, antioxidant, anticonvulsant, antitubercular, analgesic, and central nervous system depressant activities. In this study, four compounds containing 5-Fluoro-isatin thiosemicarbazone with methoxyphenyl or methoxyphenyl in different positions and zinc complexes were evaluated based on their biological activities. Compound 2 was the strongest compound affecting gramnegative bacteria compared to the other compounds. Also, this compound indicated better antimicrobial activity than positive control antibiotics. Besides, compound 3 was the only compound that inhibited the growth of Salmonella spp. such as Salmonella enteritidis ATCC 13076 and Salmonella typhimurium NRRLE 4463. 5-Fluoro-Isatin thiosemicarbazone and its derivatives also showed DNA protection property from moderate to good protections. Among them, compound 4 displayed the highest DNA binding affinity. These compounds possessed a capacity for utilization as drugs or drug additives based on their effects on bacteria strains and DNA binding affinity.