Browsing by Author "Altuner E."

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Antimicrobial activities and some flavonoids in extracts of some medicinal plants
(2017-07-01) Şener I.; Gür M.; Verep D.; Güney K.; Altuner E.
Origanum majorana, Melissa officinalis, Anthemis cotula and Avena sativa were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of some flavonoids were analyzed by using HPLC. It was determined flavonoids in the extracts of O. majorana, M. officinalis, A. cotula and A. sativa. As a results it was observed that O. majorana was active against Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis, Salmonella typhimurium and Pseudomonas aeruginosa, where M. officinalis was active against Enterococcus faecium and Enterococcus faecalis. On the other hand, both A. cotula and A. sativa were observed to be active against Enterococcus faecium. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels.
Antimicrobial activity and chemical composition screening of Anacyclus pyrethrum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient times. The resistance of pathogens to antibiotics became a critical problem, so researches for novel antimicrobial agents are required. Anacyclus pyrethrum (pellitory, Spanish chamomile or Mount Atlas daisy) is commonly used as a traditional medicine, therefore the antimicrobial activity of the root of this medicinal plant was investigated against 17 bacteria and 1 fungi by using disk diffusion method. The test strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy analysis. The results were presented that A. pyrethrum has antimicrobial activity against all tested microbial species except E. faecalis and S. typhimurium. Eighteen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed NMR spectra for 3D structure determination and identification.
Antimicrobial activity and chemical composition screening of Epilobium montanum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient time. The resistance of pathogen to antibiotic is became a critical problem, so novel antimicrobial agent related research is required. Epilobium montanum related antimicrobial research doesn’t exist, therefore root of this medicinal plant investigation was applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial species include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy. The results were presented that E. montanum has antimicrobial activity against all tested microbial species except E. coli. Seventeen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed by NMR spectra for 3D structure determination and identification.
Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme
(2014-01-01) Altuner E.; Canli K.; Akata I.
The anti-infective activities of plant-derived secondary metabolites are under investigation in recent years due to accelerating antibacterial and antifungal resistance rates of microorganisms. In this study, antimicrobial activity of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme were screened against Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria monocytogenes ATCC 7644, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disk diffusion method. It is observed that ethanolic extract of the moss samples have antimicrobial activity against several gram positive and gram negative microorganisms tested. But antimicrobial activity of D. polysetum is notable especially against S. carnosus. These results are the very first data about the antimicrobial activity of C. cuspidata and D. polysetum.
Comparison of Antimicrobial Activity of Allium sativum Cloves from China and Taşköprü, Turkey
(2018-01-01) Yetgin A.; Canli K.; Altuner E.
In this study, antimicrobial activities of two different samples of Allium sativum L. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species of Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, and Staphylococcus were used for testing antibacterial activity. In addition, antifungal activity against Candida albicans was also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.
Determination of some flavonoids and antimicrobial behaviour of some plants’ extracts
(2017-07-01) Gür M.; Verep D.; Güney K.; Güder A.; Altuner E.
C. sativa, C. intybus, L. stoechas, V. officinalis and G. glabra plants were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of eight flavonoids were analysed by using HPLC. It was found that C. sativa is active against C. albicans, E. faecalis, S. enteritidis and S. typhimurium with MIC values of 26.02 µg/mL, 13.01 µg/mL, 416.25 µg/mL and 832.50 µg/mL respectively, where C. intybus is active against C. albicans and E. faecalis, with MIC values of 13.01 µg/mL and 6.50 µg/mL, respectively. On the other hand, L. stoechas and V. officinalis were observed to be active against only S. enteritidis with MIC values of 52.03 µg/mL and 26.02 µg/L respectively, where G. glabra was active against only E. faecalis, with a MIC value of 52.03 µg/mL. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels. But the activities against C. albicans and E. faecalis is noteworthy. The flavonoids were determined at different amounts in extracts.
Determination of the chemical composition and antimicrobial activity of Frankenia hirsuta
(2017-12-24) Canli K.; Şimşek Ö.; Yetgin A.; Altuner E.
Frankenia hirsuta is widely located in Turkey, but the antimicrobial potential and biochemical composition analysis of it weren’t determined yet. By using the disk diffusion method, the susceptibility of 17 bacteria and 1 fungi were analyzed, which included Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera. 0.8, 1.5 and 3.1 mg of samples were prepared by using absolute ethanol. The bioactive composition of the plant extract was determined by gas chromatography- mass spectroscopy, and National Institute of Standards and Technology library was used for the mass spectra analysis. The results showed that F. hirsuta had antimicrobial activity against all of the studied micro-organisms except E. aerogenes and E. coli. Several active metabolites were identified, but some composition of this sample didn’t match with the library. These results are the first report for the antimicrobial potential and biochemical composition of F. hirsuta.
Effect of high hydrostatic pressure on the profile of proteins extracted from Betula pendula pollens
(2014-10-02) Altuner E.; Çeter T.; Alpas H.
High hydrostatic pressure (HHP) has high success potential in pollen protein extraction, but its effect on pollen protein profiles has not been studied yet. The aim of this study is to put forward whether HHP processing causes a change in the protein profiles extracted from pollens or not. In this study, proteins extracted from Betula pendula pollens were studied at 100, 200 and 300MPa at room temperature for 5min. In addition, the efficiency of three different extraction solvents, namely phosphate buffer saline (PBS) buffer pH 7.5, trichloroacetic acid-acetone and Tris-HCl buffer pH 8.8, was also observed, and the results were compared with the conventional pollen protein extraction procedure. As a result, it is concluded that 200MPa for 5min has extracted similar amounts of protein compared with the conventional extraction method which lasted for 24h, which lasted for 24 h. On the other hand, the application time for 200 MPa for 5 min is extremely shorter when it is compared to the conventional extraction method.
High hydrostatic pressure processing: A method having high success potential in pollen protein extraction
(2012-06-01) Altuner E.; Çeter T.; Alpas H.
Even a single peptide that is present in the pollen wall and cytoplasm could cause pollen allergy. To produce skin-prick test kits, the first step is the extraction of these molecules. In this study, Cedrus atlantica pollens were subjected to 220 and 330MPa for 10 and 30min in order to extract these molecules. After high hydrostatic pressure processing (HHPP), the total amounts of proteins (TAPs) are measured and compared with the results of the conventional extraction method (CEM). As a result, the TAPs extracted by HHPP is 18.0210 μg/mL at 220MPa for 10min, 22.5770 μg/mL at 220MPa for 30min, 23.3810 μg/mL at 330MPa for 10min and 25.9270 μg/mL at 330MPa for 30min, while this is 1.9460 μg/mL in 24h by the CEM. In addition to these results, visual pollen deformation and eruption, pollen wall and surface damage have also been observed. © 2012 Taylor & Francis.
In Vitro Antimicrobial Activity Screening of Ethanol Extract of Lavandula stoechas and Investigation of Its Biochemical Composition
(2019-01-01) Canli K.; Yetgin A.; Benek A.; Bozyel M.; Altuner E.
The aim of this study was to test antimicrobial activity of ethanol extract of Lavandula stoechas against 22 bacteria and 1 yeast. Also, biochemical composition of the extract was investigated. A wide range of Gram-positive, Gram-negative microorganisms, and multidrug resistant bacteria were selected to test the antimicrobial activity. As a result, the extract is observed to contain fenchone (bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl-, (1R)-) and camphor (+)-2-bornanone) as major components and showed antimicrobial activity against all studied microorganisms except Escherichia coli ATCC 25922 and Klebsiella pneumoniae. The results of the study present that L. stoechas is active against MDR strains too.
In vitro antimicrobial screening of cerena unicolor (Bull.) murrill (Polyporaceae fr. ex Corda)
(2012-12-01) Altuner E.; Akata I.; Canli K.
The aim of this study is to determine the antimicrobial activity of Cerena unicolor ethanolic extract against 19 bacterial and 1 fungal strains. C. unicolor samples which were collected from Yomra, Trabzon, TURKEY were extracted with ethanol. Extracts were investigated for in vitro antimicrobial activity against a wide range of strains including Bacillus cereus DSMZ 31, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durons, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumonia, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa ATCC 27853, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disc diffusion method. It is observed that ethanolic extract of C. unicolor has antimicrobial activity against several gram positive microorganims tested. As a result of the study, it could be concluded that ethanolic extract of C. unicolor is active against several microorganisms but its antimicrobial activity is notable especially against B. cereus DSMZ 31, S. carnosus MC1.B and S. epidermidis DSMZ 20044. © by PSP.
In vitro antimicrobial screening of Lycoperdon lividium and determination of the ethanol extract composition by gas chromatography/mass spectrometry
(2016-01-01) Canli K.; Altuner E.; Akata I.; Turkmen Y.; Uzek U.
The aim of this study was to investigate the antimicrobial activity of Lycoperdon lividium against 17 bacterial and 1 fungal strains and analyse the composition of ethanol extracts by GC/MS. The in vitro antimicrobial activity of L. lividium extracts having 15 mg/mL concentration was assessed against a wide range of strains by disk diffusion method. The ethanol extract of L. lividium had antimicrobial activity against several microorganism tested, but it was active especially against S. carnosus. The results obtained herein indicate that L. lividium contains several active metabolites.
Predictive modeling for 5-hydroxymethylfurfural formation by some application conditions of high hydrostatic pressure, namely glucose concentration and application temperature, in high glucose containing model beverages
(2018-11-01) Altuner E.; Alpas H.
High hydrostatic pressure (HHP) is a nonthermal food processing technology. Many studies have shown that HHP may be used as a process to increase the shelf life of beverages, which are inherently containing glucose or subsequently glucose added. Depending on food processing technologies and storage conditions 5-hydroxymethylfurfural (5-HMF) formation from glucose in foods occurs. There are some limitations about the amount of the 5-HMF present in foods based on World Health Organization and various International Standards, such as European Commission Directive 2007/196/EC. The aim of this study is to present the effect of glucose concentration and HHP in 5-HMF formation in model beverages with different glucose concentrations (10, 40, and 70 g/200 mL). A strong positive correlation was observed between 5-HMF formation and glucose concentration (R = 0.6055) and application temperature (R = 0.6774). Also a predictive analysis model was fitted to the 5-HMF formation response by response surface model (RSM). Practical applications: The equation obtained as a model in this study can be used to calculate the formation of 5-HMF for a glucose containing beverages processed by HHP as a response of glucose concentration and application temperature within the conditions of the study. The model was calibrated and validated on glucose containing model beverages, which are representatives of some high glucose containing beverages. HMF content is an important parameter and should be included as one of the quality parameters for commercial beverages. Because of that, this equation has an importance especially for the beverage producers, who process their product by HHP, to predict 5-HMF formation by controlling glucose concentration and HHP treatment conditions. Moreover, developed model can easily be adapted to the standard procedures of academic and industrial applications.
The effect of high hydrostatic pressure processing on the extraction, retention and stability of anthocyanins and flavonols contents of berry fruits and berry juices
(2013-10-01) Altuner E.; Tokuşoǧlu O.
Anthocyanins and flavonols are types of phenolic compounds. Previous researches showed that phenolic compounds are relevant to the quality of plant-derived foods and beverages. Any food-processing technologies, which can preserve phenolic compounds after processing, should be taken into account for increasing the food quality. High hydrostatic pressure processing (HHPP) is a novel nonthermal process. There are some researches about the effect of HHPP on total phenolic content, total monomeric anthocyanins, kaempferol, myricetin and quercetin contents of berry fruits and berry juices in the literature. These studies showed that HHPP could possibly have a success potential in increasing the extraction, retention and stability of anthocyanins and flavonols contents of berry fruits and berry juices by preserving phenolic compounds after food processing. The aim of this review is to put forward how high hydrostatic pressure processing affects extraction, retention and stability of anthocyanins and flavonols of berry fruits and juices. International Journal of Food Science and Technology © 2013 Institute of Food Science and Technology.