Browsing by Author "Altun, G."

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    Web of Science
    The potential protective effects of melatonin and omega-3 on the male rat optic nerve exposed to 900?MHz electromagnetic radiation during the prenatal period
    (2023.01.01) Tuefekci, K.K.; Kaplan, A.A.; Kaya, A.; Alrafiah, A.; Altun, G.; Aktas, A.; Kaplan, S.
    Background: Due to children and adolescents' widespread use of electronic devices, researchers have focused on pre-and early postnatal electromagnetic field (EMF) exposure. However, little is known about the effects of EMF exposure on the optic nerve. The aim of study was to investigate the changes occurring in the optic nerve and the protective effects of melatonin (mel) and omega 3 (?-3) in rats.Methods: Thirty-five pregnant rats were divided into seven groups, Cont, Sham, EMF, EMF + melatonin (EMF + Mel), EMF + ?3, Mel, and ?3. The EMF groups were exposed to 900 megahertz (MHz) EMF daily for two hours during pregnancy. After the experiment, the right optic nerve of each offspring rat was removed and fixed in glutaraldehyde. Thin and semi-thin sections were taken for electron microscopic and stereological analyses. Myelinated axon numbers, myelin sheath thicknesses, and axonal areas were estimated using stereological methods.Results: The groups had no significant differences regarding mean numbers of axons, mean axonal areas, or mean myelin sheath thicknesses (p > 0.05). Histological observations revealed impaired lamellae in the myelin sheath of most axons, and vacuolization was frequently observed between the myelin sheath and axon in the EMF-exposed group. The Mel and ?-3-treated EMF groups exhibited well-preserved myelinated nerve fibers and intact astrocytes and oligodendrocytes.Conclusions: At the ultrastructural level, Mel and ?3 exhibits a neuroprotective effect on the optic nerve exposed to prenatal EMF. The protective effects of these antioxidants on oligodendrocytes, which play an essential role in myelin formation in the central nervous system, now require detailed investigation.
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    Scopus
    Uterus stereology
    (Nova Science Publishers, Inc., 2024) Kaya, A.; Altun, G.; Tüfekçi, K.K.; Kaplan, A.A.; Kipanyula, M.J.; Kaplan, S.
    Infertility is a growing global health problem with important socio-psychological consequences. The main causes of infertility are the pathogenesis of the uterine mucosa and the uterus being unready for implantation as a result of decidualization. Despite the critical importance of the uterus for women's health and fertility, and embryo development, relatively limited information is available concerning the hormonal, cellular, and molecular mechanisms in its pathogenesis. Morphoquantitative assessments are widely used by researchers to explain the underlying causes of infertility. Quantitative evaluations provide more descriptive and precise information when observing structural changes in tissue under different conditions and comparing these between different groups. Design-based stereology provides an accurate and unbiased estimation of stereological parameters, avoiding sources of bias caused by the methodology employed. It is therefore important to use stereological methods when considering a quantitative morphological evaluation of biological objects. This chapter describes the importance of developing and disseminating the use of stereological techniques in uterine research and is intended to explain the modern stereological techniques applied to uterine tissue in methodological terms.