Browsing by Author "Akpinar E."

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5-HT7 receptors as a new target for prostate cancer physiopathology and treatment: an experimental study on PC-3 cells and FFPE tissues
(2021-06-01) Cinar I.; Sirin B.; Halici Z.; Palabiyik-Yucelik S.S.; Akpinar E.; Cadirci E.
Prostate cancer (PCa) is one of the most common types of cancer seen among men worldwide. Previous studies have demonstrated that serotonin regulates cell proliferation, migration, and invasion in vitro; the presence of 5-HT receptors in cancer cells; and the role of serotonin in tumor development. The most recently discovered of these receptors is 5-HT7 but also least characterized receptors of serotonin. The aim of this study is to investigate the existence and possible role of 5-HT7 receptors in healthy and cancerous prostate tissues and also investigate effects of receptor agonists and antagonists on PC-3 cells to evaluate potential therapeutic effects. PC-3 cells were cultured and effects of 5-HT7 receptor agonist (LP-44) and antagonist (SB-269970) were evaluated on these cells. After proliferation analyses, relative expression of apoptotic markers and 5-HT7 receptor mRNA expression levels were determined through real-time PCR. Annexin V-FITC/PI double staining and Hoechst 33258 staining assay methods were applied to determine apoptosis. Additional PCR studies were performed on healthy and cancerous prostate tissue to see existence of receptors in human samples. The viability of PC-3 cells was decreased by SB-269970 after 48 and 72 h of incubation. However, LP-44 increased PC-3 cell proliferation at all time points. In 10-6 M SB-269970 treated PC-3 cells, there was significant increase in the expression of CAS-3 (4-fold), CAS-9 (2.5-fold), BAX (1.9-fold), and Tp-53 (4.8-fold) gene mRNA levels when compared to non-treated control group. Conversely, there was a significant decrease in NF-κB (2.9-fold) and 5-HT7 receptor (3.6-fold) mRNA expression in cells treated with SB-269970 when compared to control. SB-269970 that antagonized 5-HT7 receptors also induced apoptosis in Annexin V-FITC/PI double staining assay and Hoechst 33258 staining assays when compared with other groups. In human samples, 5-HT7 receptor mRNA expression was approximately 200-fold higher than that of heathy ones. In this study, for the first time, the 5-HT7 receptor antagonist SB-269970 has been shown to inhibit proliferation in PC-3 cells and to be associated with an apoptosis-inducing effect. These results suggest blocking 5-HT7 receptors can be a novel therapeutic target for the treatment of prostate cancer.
Appreciation to Referees, 2023
(2023-12-01) Abdelwahed A.; Akpinar E.; Anderer A.; Andradottir S.; Arici H.E.; Athaide G.; Bijvank M.; Bommaraju R.; Boute R.; Bridges E.; Cetinkaya S.; Chark R.; Chatterjee S.; Chen J.; Chen J.; Chen Y.; Chen Z.; Cheng Y.; Conejo A.; Cornelius P.; Dai B.; Dai W.; Davies P.; Dixon M.; Dong B.; Dong X.; Erjavec J.; Feng G.; Ghaffarzadegan N.; Guo X.; Han S.; Hao S.; He S.; Ho K.; Jalloul M.; Jeong H.; Jia L.; Jia M.H.; Jin J.; Jin Z.; Karniouchina E.; Khademi A.; Kim C.; Kim S.W.; Klapp M.; Kong L.U.; Kuo Y.H.; Kwon C.; Lee M.K.; Leus R.; Li C.; Li S.; Lim S.; Liu B.; Liu H.; Liu L.; Liu R.; Liu S.; Liu S.; Liu S.; Liu X.; Liu Y.; Lu Y.; Mao H.; Meng L.; Messinger P.; Najda-Janoszka M.; Nguyen J.; Nusrat F.; Ouyang H.; Pang J.M.; Pansari A.; Paquette J.; Pourghannad B.; Pun H.; Ratchford B.; Ratchford M.; Reed S.; Ren H.; Roy A.; Secchi E.; Shan Z.; Shehadeh K.; Shin J.; Song B.; Steimle L.; Su L.; Sun X.; Sun Y.; Tereyagoglu N.; Tuzovic S.; van Droogenbroeck E.; Visnjic I.; Wang D.; Wang R.; Wang S.; Wang Z.; Xu C.; Xu D.; Xu L.
Effects of simultaneous versus post exposure epigallocatechin-3-gallate treatment on aluminum induced neurotoxicity in rat hippocampus: A multi-approach study
(2023-03-01) Palabiyik-Yuceli̇k S.S.; Zeybek N.D.; Cinar I.; Akpinar E.; Bahador Zırh E.; Si̇pahi̇ H.; Halici Z.
Chronic aluminium(Al) exposure can affect the antioxidant and glutaminergic systems through N-methyl-D-aspartate receptors (NMDAR). This study was aimed to investigate the neurotoxic effect of Al through different mechanisms in rat hippocampus and to evaluate the protective role of epigallocatechin gallate (EGCG), a well-known antioxidant, with simultaneous administration of Al,as well as post-treatment after Al exposure.For this purpose, aluminum chloride(AlCl3) was administered simultaneously with two different EGCG doses for 8 weeks as the first part of the study.In the second part of the study, after 4 weeks of AlCl3 pre-administration, two different EGCG doses were also administered during four additional weeks as post-treatment.Al administration led to oxidative stress and increased acetylcholinesterase levels.NMDAR subunit mRNA expressions were down-regulated by Al, which was apparent in NMDAR1/2B subunits.Simultaneous EGCG treatment has shown a better neuroprotective effect in terms of these mechanisms and represents novel approach for the prevention of neurodegenerative diseases likely to be induced by Al.
Investigation of the effects of probiotics on sub-chronic neonicotinoid toxicity in rats
(2021-10-01) Sevim C.; Akpinar E.; Tsatsakis A.; Yildirim S.; Tzatzarakis M.; Vardavas A.I.; Vardavas C.I.; Kara M.; Gul Z.
Probiotics have been shown to have positive effects when it comes to combating various health issues when consumed, preventing even the absorption of environmental toxins. One of the main environmental toxins encountered today is pesticide residues. Neonicotinoids, widely applied today in countries that have approved of them, are a known class of insecticides with an excellent and effective potency. Neonicotinoids have been shown to cause various toxic effects, either acutely or chronically, on human health and on beneficial insects when exposed. To clarify the assumption that probiotics could counteract these toxic effects, especially on vital organs, the probiotic yeast “Saccharomyces boulardii” (S. boulardii) was tested against the neonicotinoids, acetamiprid (ACE) and imidacloprid (IMI), as it has outstanding physiological and metabolic properties. The results obtained from the studies indicated that although ACE and IMI induced liver, kidney, brain and bowel damage, there was a considerable level of protection by the dietary supplementation of S. boulardii, as it reduced the absorption of these insecticides.