Browsing by Author "Akata I."

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A new pollen-derived microcarrier for pantoprazole delivery
(2017-02-01) Akyuz L.; Sargin I.; Kaya M.; Ceter T.; Akata I.
Plant-derived carriers have emerged as promising materials for drug encapsulation. Especially, sporopollenin microcapsules extracted from diverse pollen species have been proved to be effective drug carriers due to their biocompatibility, homogeneity in size, resistance to harsh chemical conditions and high thermal stability. Here in this study, sporopollenin microcapsules were isolated successfully from the pollens of a common tree (Corylus avellana, the European hazelnut) and used as a carrier for pantoprazole (PaNa) (a proton pump inhibitor). The drug entrapment efficiency was recorded as 29.81%. SEM micrographs clearly showed the drug was loaded into the microcapsules through the apertures of microcapsule and also some drugs were adsorbed on the surface of microcapsules. FT-IR spectra analysis confirmed the drug loading. Thermogravimetric analysis revealed that thermal stability of PaNa was enhanced by encapsulation. In vitro release studies showed that PaNa-loaded sporopollenin microcapsules exhibited better release performance than the control. C. avellana sporopollenin microcapsules can make an efficient carrier for delivery of PaNa.
Antimicrobial activity and chemical composition screening of Anacyclus pyrethrum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient times. The resistance of pathogens to antibiotics became a critical problem, so researches for novel antimicrobial agents are required. Anacyclus pyrethrum (pellitory, Spanish chamomile or Mount Atlas daisy) is commonly used as a traditional medicine, therefore the antimicrobial activity of the root of this medicinal plant was investigated against 17 bacteria and 1 fungi by using disk diffusion method. The test strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy analysis. The results were presented that A. pyrethrum has antimicrobial activity against all tested microbial species except E. faecalis and S. typhimurium. Eighteen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed NMR spectra for 3D structure determination and identification.
Antimicrobial activity and chemical composition screening of Epilobium montanum root
(2017-07-01) Canli K.; Yetgin A.; Akata I.; Altuner E.
Medical herbs have many bioactive component and they are used in microbial treatment since ancient time. The resistance of pathogen to antibiotic is became a critical problem, so novel antimicrobial agent related research is required. Epilobium montanum related antimicrobial research doesn’t exist, therefore root of this medicinal plant investigation was applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial species include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy. The results were presented that E. montanum has antimicrobial activity against all tested microbial species except E. coli. Seventeen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed by NMR spectra for 3D structure determination and identification.
Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme
(2014-01-01) Altuner E.; Canli K.; Akata I.
The anti-infective activities of plant-derived secondary metabolites are under investigation in recent years due to accelerating antibacterial and antifungal resistance rates of microorganisms. In this study, antimicrobial activity of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme were screened against Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria monocytogenes ATCC 7644, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disk diffusion method. It is observed that ethanolic extract of the moss samples have antimicrobial activity against several gram positive and gram negative microorganisms tested. But antimicrobial activity of D. polysetum is notable especially against S. carnosus. These results are the very first data about the antimicrobial activity of C. cuspidata and D. polysetum.
Antimicrobial screening of mnium stellare
(2015-04-11) Canli K.; Altuner E.M.; Akata I.
Many plants contain active substances that are known to be effective in both enhancing the wound healing process and lowering the incidence of wound infections. Previous studies have shown that bryophytes produce a variety of secondary metabolites that present pharmaceutical activities including antimicrobial activity against various pathogenic bacteria and fungi. The aim of this study was to investigate the antimicrobial activity of Mnium stellare against 17 bacterial and 1 fungal strains. Our present study has shown that the ethanol extract of M. stellare has antimicrobial activity against several Gram positive and Gram negative microorganism tested, but its antimicrobial activity is notable especially against B. subtilis, S. typhimirium, S. aureus, S. carnosus, and S. epidermidis. These results are the very first report of the antimicrobial activity of M. stellare.
BIOCHEMICAL COMPOSITION, AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SALVIA FRUTICOSA, AN ETHNOMEDICINAL PLANT
(2023-01-01) Bozyel M.E.; Canli K.; Benek A.; Simsek O.; Akata I.; Altuner E.M.
In Vitro antimicrobial activity screening of xylaria hypoxylon
(2016-01-01) Canli K.; Akata I.; Altuner E.M.
Background: Fungi have a potential of using both as nutritive and medicinal food stuff. Because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. The aim of this study is to determine the in vitro antimicrobial activity of Xylaria hypoxylon, which were collected from Yomra, Trabzon, Turkey. Materials and Methods: X. hypoxylon samples were air dried and extracted by using ethanol. Antimicrobial activity of X. hypoxylon ethanol extracts were investigated against 21 bacterial and 2 fungal strains, namely, Bacillus subtilis DSMZ 1971, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Candida albicans DSMZ 1386, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumoniae, Listeria innocula, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disk diffusion method. Results: It is observed that ethanol extracts of X. hypoxylon has antimicrobial activity against several Gram positive and Gram negative microorganisms tested. As a result of the study, an antimicrobial activity of X. hypoxylon found against most of strains used in the study. Conclusion: The results of our study clearly puts forward that X. hypoxylon could have a possible medicinal use.
In vitro antimicrobial screening of Aquilaria agallocha roots
(2016-01-01) Canlı K.; Yetgin A.; Akata I.; Altuner E.M.
Background: It was previously shown that some parts of Aquilaria agallocha, which is commonly known as oud or oodh, such as roots have been used as a traditional medical herbal in different countries. In Turkey A. agallocha is one of the ingredients while preparing famous Mesir paste, which was invented as a medicinal paste and used from the Ottoman period to now at least for 500 years. The identification the in vitro antimicrobial activity of ethanol extract of A. agallocha roots is main purpose of this analysis. Materials and Methods: By using 17 bacteria and 1 fungi, which include Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera, the activity of A. agallocha root extracts were analysed by the help of the disk diffusion method, that is one of the methods commonly used to determine antimicrobial activities. Results: As a result of the study it was observed that ethanol extracts of A. agallocha roots have a clear antimicrobial activity against nearly all microorganism used in the study, but only two bacteria namely E. coli ATCC 25922 and S. typhimurium SL 1344. Conclusion: According to the disk diffusion test results it may be possible to propose that A. agallocha roots should have a medicinal uses especially against E. faecium, L. monocytogenes ATCC 7644, B. subtilis DSMZ 1971, C. albicans DSMZ 1386, S. epidermidis DSMZ 20044 and S. aureus ATCC 25923.
In vitro antimicrobial screening of cerena unicolor (Bull.) murrill (Polyporaceae fr. ex Corda)
(2012-12-01) Altuner E.; Akata I.; Canli K.
The aim of this study is to determine the antimicrobial activity of Cerena unicolor ethanolic extract against 19 bacterial and 1 fungal strains. C. unicolor samples which were collected from Yomra, Trabzon, TURKEY were extracted with ethanol. Extracts were investigated for in vitro antimicrobial activity against a wide range of strains including Bacillus cereus DSMZ 31, Bacillus subtilis ATCC 6633, Candida albicans ATCC 10231, Enterobacter aerogenes ATCC13048, Enterococcus durons, Enterococcus faecalis ATCC 29212, Enterococcus faecium, Escherichia coli ATCC 25922, Escherichia coli CFAI, Klebsiella pneumonia, Listeria monocytogenes ATCC 7644, Pseudomonas aeruginosa ATCC 27853, Salmonella enteritidis ATCC 13075, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923, Staphylococcus carnosus MC1.B, Staphylococcus epidermidis DSMZ 20044 and Streptococcus agalactiae DSMZ 6784 by using the disc diffusion method. It is observed that ethanolic extract of C. unicolor has antimicrobial activity against several gram positive microorganims tested. As a result of the study, it could be concluded that ethanolic extract of C. unicolor is active against several microorganisms but its antimicrobial activity is notable especially against B. cereus DSMZ 31, S. carnosus MC1.B and S. epidermidis DSMZ 20044. © by PSP.
In vitro antimicrobial screening of Lycoperdon lividium and determination of the ethanol extract composition by gas chromatography/mass spectrometry
(2016-01-01) Canli K.; Altuner E.; Akata I.; Turkmen Y.; Uzek U.
The aim of this study was to investigate the antimicrobial activity of Lycoperdon lividium against 17 bacterial and 1 fungal strains and analyse the composition of ethanol extracts by GC/MS. The in vitro antimicrobial activity of L. lividium extracts having 15 mg/mL concentration was assessed against a wide range of strains by disk diffusion method. The ethanol extract of L. lividium had antimicrobial activity against several microorganism tested, but it was active especially against S. carnosus. The results obtained herein indicate that L. lividium contains several active metabolites.