Browsing by Author "Akarsu, S.A."

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IMMUNOFLUORESCENCE AND BIOCHEMICAL INVESTIGATION OF THE PROTECTIVE EFFECTS OF NARINGIN AND DIOSMIN ON THE FREEZABILITY OF MERINO RAM SEMEN
(2024.01.01) Ömüer, A.D.; Kaynar, O.; Ileritürk, M.; Özkaraca, M.; Öztürk, A.E.; Bodu, M.; Sahin, I.N.; Akarsu, S.A.
BACKGROUND: Various antioxidant substances are added to sperm extenders to protect spermatozoa against oxidative stress and cryodamage. OBJECTIVE: To investigate the effects of the flavonoid diosmin (DIO) and a flavanone glycoside naringin (NAR) on the freezability of ram semen. MATERIALS AND METHODS: In this study, six Merino rams were used during the breeding season. The ejaculates were pooled after collection from the rams. Pooled ejaculates were divided into six groups: control, NAR 1 mM, NAR 2 mM, NAR 4 mM, DIO 2 mM, and DIO 4 mM, and then diluted with a TRIS-based diluent. The pooled semen was equilibrated, placed in 0.25 mL pipettes with 10 x 10(7) sperm cells in each pipette, and frozen in liquid nitrogen vapor. After 24 h, the pipettes were thawed at 37 degrees C for 25 s and analyzed in terms of spermatological parameters. RESULTS: The highest plasma membrane integrity ratio was found in the DIO 4 mM group, whereas a statistically significant difference was found between the NAR 1 mM and NAR 2 mM groups (p<0.05). While the DIO 4 mM group had the highest acrosome integrity rate, a statistically significant difference was found between the other groups (p<0.05). Mitochondrial activity was the highest in the NAR 4 mM, DIO 4 mM and DIO 2 mM groups (p<0.05). In the analysis of the sperm membrane lipid profile, it was observed that the DIO group had the highest lipid-phospholipid ratio. In sperm membrane protein profile analysis, it was found that both additives exerted protective effects at different levels. The highest total protein content was seen in the DIO 4 mM and NAR 4 mM groups. 8hydroxydeoxyguanosine (8-OhDG) positivity was more common in the control group than in the DIO and NAR groups. Cu-Zn superoxide dismutase (SOD) expression was lower in the control group and more intense in all other groups. Positive results were especially observed in the acrosome of the sperm cells. CONCLUSION: The addition of NAR and DIO to the ram semen extender increased the quality of sperm parameters after the freeze-thaw process.
Investigation of the subchronic effects of low-dose pesticide mixture on rat testes
(2023.01.01) Ömür, A.D.; Sevim, C.; Saglam, Y.S.; Bolat, I.; Genç, S.; Yeni, Y.; Aydin, M.A.; Sunar, S.; Koçak, G.; Akarsu, S.A.
Pesticides are chemical agents used against living things such as insects, rodents, and weeds that cause toxicity in various tissues, including reproductive organs. In order to increase the effectiveness of pesticides, active ingredients are mixed with various formulations. The current study tried to determine the effects of pesticides and their mixtures on testicular tissue in rats. For this purpose, chlormequat chloride (CCC), pirimiphos methyl (PMM), glyphosate ( GLY), tebuconazole (TBZ), chlorpyrifos methyl (CPM), deltamethrin (DLM), and their mix (acceptable daily intake (ADI) and ADIx10) were administried to Sprague-Dawley rats for 90 days. As a result of the examinations, the ADIx10 group showed the most damaging effect by showing a statistical difference in terms of sperm motility and membrane integrity. The GLY group had a similar effect on membrane integrity as the ADIx10 group, while it had a moderately detrimental effect on motility. Degenerations of spermatocytes, necrosis, and edema in intertubular spaces were observed in all pesticide groups. Similarly, 8-hydroxyguanosine (8-OHdG) expression and Caspase-3 expression were moderate in all pesticide groups. Moreover, 8-OHdG expression and Caspase-3 expression were higher in the ADI and ADIx10 groups compared to other groups. As a result, pesticides and their mixtures cause histopathological changes in the testicular tissue of rats and decrease sperm quality.