Browsing by Author "İrfan ÇINAR"

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Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells
(2022-01-01) Selin AKSAK KARAMEŞE; Mustafa ÇİÇEK; İrfan ÇINAR
Aim: Today, breast cancer is a disease that is encountered commonly in women with limited options for treatment. It is needed to find new agents that can be effective in preventing or managing this disease. It has been demonstrated that gossypin inhibits tumor growth. In our study, it has been targeted to examine the effects of gossypin regarding both anticancer activity and apoptosis in MCF-7 cells. Material and Method: MCF-7 cells were treated with different doses of gossypin and with 50 µM cisplatin for 24, 48, and 72 hours. The MTT analysis, Caspase-3, Caspase-9, and Nf-KB mRNA expressions of those MCF-7 cells which were treated with gossypin were also conducted in order to evaluate the apoptosis or necroptosis-induced cell death. Results: In MTT experiments, it has been observed that the administration of 100 µM dose of gossypin had similar effects to the routine cisplatin administration, caused a significant decrease in cell proliferation, and increased apoptosis in the evaluations of Hoechst staining and morphology. It has been put forth that gossypin decreases the expression of CASP-3 and CASP-9 mRNA and increases the expression of NF-kB.Conclusion: Our results demonstrate that for the breast cancer cells, the 100 µM of gossypin positively affects cell death pathways due to apoptosis.
Gossypin’in Kato-III Hücrelerindeki Anti-Proliferatif ve Apoptotik Etkilerinin Belirlenmesi
(2021-04-01) Harun ÜN; Rüstem Anıl UĞAN; İrfan ÇINAR
Gastrik karsinoma yüksek morbidite ve mortalite ile insanlarda ve hayvanlarda görülen bir kanser türüdür. Gossypin Hibiscus vitifolius'tan izole edilen doğal bir flavonoid grubunda doğal bir maddedir. Bu çalışmada gossypin’in Kato-III gastrik karsinoma hücrelerinde anti-proliferatif ve apoptotik etkilerinin araştırılması amaçlanmıştır. Kato-III hücreleri uygun ortamlarda kültüre edildi ve gossypin’in etkin doz aralığı (5-100 μg/ml) ve hücre canlılığı üzerindeki etkileri MTT testi ile 24, 48 ve 72. saatlerde tespit edildi. RT-PCR yöntemi ile kaspaz-3, 9 ve NF-kB gen ekspresyonları belirlendi. Floresan boyama tekniği ile apoptoz seviyeleri tespit edildi. Sisplatin pozitif kontrol grubu olarak deney eklendi. Gossypin’in Kato-III hücre proliferasyonunu doza ve zamana bağlı olarak azalttığı tespit edildi. Gossypin kaspaz-3 ve kaspaz-9 seviyelerini artırırken NF-kB seviyelerini inhibe ettiği ve sonuçların sisplatin ile benzerlik gösterdiği tespit edildi. Hücre morfolojik analiz ve floresan boyama sonuçlarına göre gossypin 100 μg/ml dozunda apoptozu en fazla artıran grup olduğu gösterilmiştir. Sonuç olarak gossypin’in gastrik karsinoma hücrelerinde apoptozu aktive ettiği ve tedavide potansiyel bir aday olabileceği ortaya konmuştur.
Impact of gossypin on gene expression of HSP60 and HSP70 in different cancer cell lines
(2022-03-01) Ebubekir DİRİCAN; İrfan ÇINAR
Purpose: The aim of this study is to evaluate the impact of gossypin on the expression level of heat shock proteins (HSPs) genes in different cancer cells. Materials and Methods: Cells were grown under standard culture conditions. Cancer cells were treated with different concentrations (5-100 µg/ml) of gossypin and cisplatin (50 µM) as positive control. Cell viability and effective dose range (5-100 µg/ml) of gossypin were determined by MTT at 24, 48 and 72 hours. After RNA isolation and cDNA synthesis, HSP60 and HSP70 gene expression levels were analyzed using RT-PCR. For gene expression analysis, the 2-∆∆ct method was used. Results: According to the MTT results, 25-50-100 µg/ml of gossypin doses were found effective on HSP60 and HSP70 gene expression levels in the cancer cell lines. Gossypin affected with dose-dependently the expression of HSP60 and HSP70 in the three cell lines. In the three cell lines, 50 µg/ml and 100 µg/ml of gossypin doses significantly reduced the expression of HSP60 and HSP70 compared to control group. Conclusion: Our results strongly supported the anticarcinogenic effect of gossypin at various doses in different cell lines. However, we believe that further in vivo research and human studies are needed. Our findings suggest that gossypin could be suitable candidate agent for further investigation to develop new strategies for the prevention and/or treatment of different cancer types.
Investigation of the effect of gossypin on MMP-2 and MMP-9 genes in prostate cancer cells
(2022-09-30) Ebubekir DİRİCAN; İrfan ÇINAR
Purpose: The aim of this study is to explore the effects of gossypin on matrix metalloproteinases -2 (MMP-2) and MMP-9 genes in prostate cancer cells. Materials and Methods: PC3 cells were grown in culture and treated with three different concentrations of gossypin (25-50-100 µg/ml) and cisplatin (50 µM) as a positive control. First, RNA isolation was performed. Then, cDNA synthesis was performed and RT-PCR was used to evaluate mRNA expression levels of MMP-2 and MMP-9 genes.Results: Gossypin decreased MMP-2 and MMP-9 mRNA expression in prostate cancer cells in a concentration-dependent manner. Three concentrations (25-50-100 µg/ml) of gossypin in PC3 cells reduced the mRNA expression of the MMP-2 gene. While the fold change value of MMP-2 gene expression was 0.3482 ± 0.040 in the 100 µg/ml gossypin group, it was 1.007 ± 0.1425 in the control group. In addition, 50 µg/ml and 100 µg/ml concentrations of gossypin decreased the mRNA expression of the MMP-9 gene. The expression level of the MMP-9 gene in prostate cancer cells was 0.4740 ± 0.038 in the 100 µg/ml gossypin administered group, while it was 1.009 ± 0.1687 in the control group. There was a positive correlation between the expressions of the MMP-2 and MMP-9 genes.Conclusion: According to the results obtained, it is seen that gossypin reduces the expression of MMP-2 and MMP-9 genes in prostate cancer cells and the effects of gossypin on other genetic and epigenetic mechanisms in cancer need to be investigated to reveal the anti-cancer.
Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration
(2023-07-01) Büşra DİNÇER; Azat ÇEKDAR GÜNDOĞMUŞ; İrfan ÇINAR
Aim: Oxidative stress has been a significant factor in wound-healing pathophysiology for a long time. Antioxidants, especially natural compounds, have recently been emphasized in instructions for wound healing treatments. Jaceosidin (JACE), a flavone derived from Artemisia princeps, is a potent antioxidant. This study aims to investigate JACE’s anti-inflammatory and antioxidant properties and its capacity to improve the effects of in vitro wound healing. Methods: Wound healing activities have been tested using cell proliferation and migration in vitro assays in the mouse fibroblast cell line L929. The concentration of hydrogen peroxide (H2O2-0.5 mM) has been used to induce the oxidative stress model. Tumor necrosis factor-alpha (TNF-α) and nuclear factor (NF-) have been investigated as inflammatory indicators. Antioxidant activity has been checked using total antioxidant status (TAS) and total oxidant status (TOS) tests. Results: JACE has significantly increased the proliferation of fibroblasts dose-dependent manner. It has enhanced the cell migration rate of fibroblasts compared with the H2O2 group. JACE at a concentration of 50 and 100 μM has significantly decreased TOS and oxidative stress index (OSI) levels and increased TAS levels. The anti-inflammatory mechanism of JACE has involved down-regulation of the mRNA expressions of the NF- and TNF-α in a dose-dependent manner. Conclusions: JACE has beneficial impacts on fibroblast viability and migration qualities through antioxidative actions and down-regulating proinflammatory cytokines through anti-inflammatory effects to promote wound healing. The present study shows that JACE may help to increase the range of available treatments for woundhealing by reducing inflammation and oxidative stress.
Role of Endothelin 1 on Proliferation and Migration of Human MCF-7 Cells
(2020-10-01) Zafer BAYRAKTUTAN; Muhammet ÇELİK; Arzu BİLEN; İrfan ÇINAR; Muhammed YAYLA
Objective: The aim of this study was to explore the role of endothelin 1 (ET-1) in human breast cancer proliferation and migration and antagonism of endothelin receptor A (ETAR) and endothelin receptor B (ETBR) by using the non-selective dual ETA/ETB receptor antagonist bosentan and determine its anti-proliferative, anti-metastatic, and apoptotic effects demonstrated by nuclear factor kappa B (NF-kB), vascular endothelial growth factor (VEGF), Caspase 3 and Caspase 9 expression on endothelin-induced proliferation of MCF-7 cell line in vitro.Materials and Methods: A total of 8,000 cells were seeded into e-plates 24 hours after the cells were incubated with or without 10-4 M BOS (1 hour before ET-1 treatment); 10-7, 10-8, and 10-9 M ET-1 for 1-4 days.Results: Whether ET-1 is present or not in the tumor area, bosentan exerts anti-proliferative effect on breast cancer. However, ET-1 and bosentan group showed important inhibitory effect on tumor migration compared to bosentan alone, which can be attributed to increased activity of ET-1 axis in the presence of ET-1. The imbalance among the NF-kB, caspases, and VEGF, which are predictive factors of carcinogenesis significantly improved after bosentan administration.Conclusion: Our study definitely demonstrated ET-1 and its critical role in cancer progression with apoptotic and anti-apoptotic pathways (NF-κB) and VEGF expression, and migration analyses were also performed. The second major finding was that bosentan inhibited ET-1-mediated effects on tumor proliferation and migration.