Browsing by Author "Çeter, T."

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A Comprehensive Overview of the Effects of Saffron Extract and Its Ingredients on Neurologic, Diabetes, Cardiovascular and Gastrointestinal Diseases: Biological Studies Using CRISPR-Cas9, Docking, Omics and Bioinformatics Approaches
(2023.01.01) Çeter, T.; Mollaamin, F.; Monajjemi, M.
Although the types of scientific tests used change over time, saffron intake has been found to reduce symptoms of diabetes, cholesterol, Alzheimer's, depression, and many other diseases. The purpose of this study was to provide safe and well-controlled clinical tests to clearly analyze the potential mechanisms of the effects of saffron. Biological markers after saffron use are measured in relationship with the predicted health results, so that the results of various studies can be compared and explicated. Since the natural propagation of saffron does not occur quickly and its corms can be manually dug up, separated and replanted, biotechnological methods can increase the ability to generate large amounts of various saffron compounds, such as crocin, picrocrocin, crocetin and safranal, in vitro. Since pathogenic plants cause crop losses in agriculture environment, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 9 (Cas9) can help with biotic and abiotic problems via a crop molecular reproduction plan, with improvement of the genes' responses to issues induced through viruses, fungi, and bacteria. Quantitative Structure Activity Relationship (QSAR) methods can be used for determining the maintenance times of ingredients for saffron extract through analyzing solid phase micro-extraction gas chromatography-mass spectrometry (SPMEGC-MS). QSAR is an accurate method for detecting relationships between the molecular properties of chemical reactions and biological systems. Bioinformatics tools can help the mechanism of the transcriptome of saffron based on the structural foundation of the flavor, color biogenesis, genomic establishment and biological gynoecium of saffron. The data extracted from bioinformatics websites can be used for constructing biological routes containing the biosynthesis of main ingredients of saffron, i.e., crocin, crocetin, safranal, picrocrocin. Omics-based technologies have been extensively discussed within biology and can be used for saffron. These studies have given rise to concepts for the better understanding of saffron growth and its therapeutic action. Molecular docking simulation has also been discussed to find the location of safranal inside lysozyme. The interaction of safranal with molecular biology was discussed by mixing approaches including CRISPR/Cas9, docking, bioinformatics and omics. This provides a new approach that is in with the results obtained
Determination of potentially toxic metals and natural radionuclides in airborne pollens produced different urban environments in Turkey and health risk assessment
(2024) Turhan, Ş.; Çeter, T.; Altuner, E.M.; Karabıcak, S.; Çeter, S.; Bıyıklıoğlu, O.; Aktaş, Ş.; Kurnaz, A.
Air pollutants are associated with potentially toxic metals (PTMs) and natural and/or artificial radionuclides, which can pose a major threat to human and environmental health. Pollens can be utilized as a bioindicator to determine the level of air pollution in urban areas. In this study, the concentrations of PTMs and natural radionuclides in 35 airborne pollen samples of 22 species belonging to Pinaceae, Cupressaceae, Araucariaceae, Betulaceae, Salicaceae, and Oleaceae families grown in different urban areas in Turkey were determined using an energy-dispersive X-ray fluorescence spectrometry. For the first time, non-carcinogenic and radiologic health risk assessments for adults were done, estimating hazard index (HI) and annual effective dose (AED), respectively. The concentrations of Fe, Mn, Zn, Ti, Sr, Cu, Ni, Co, Cr, V and Pb analyzed in airborne pollen samples varied from 52.1 to 3078.0, 26.1 to 159.6, 15.6 to 199.7, 9.1 to 282.2, 1.0 to 128.4, 5.0 to 40.1, 5.4 to 23.6,
Identification of Marker Molecules in Aqueous Plant Extracts Affecting the Gold Nanostructures' Morphology and Size
(2024.01.01) Elgaleidh, M.A.M.; Tepe, H.D.; Doyuk, F.; Çeter, T.; Yazgan, I.
This work was performed as a comparative study using nine different aqueous pollen grain extracts from eight different genera (Juniperus, Biota, Cupressus, Abies, Pinus, Cedrus, Populus and Corylus) to synthesize gold nanostructures (AuNSs) to understand if there is any possible marker that helps to predict the final morphology and size of the AuNSs. Principal component analysis (PCA) revealed that Apigenin and Pinoresinol compounds are the marker molecules in determination of the AuNSs physical characteristics while total protein, reducing carbohydrate, flavonoid and phenol contents did not show any statistically meaningful outcome. The "dominancy hypothesis" was tested by paying attention to the most concentrated phenolic acids and flavonoids in the control of AuNSs morphology and size, for which correlation analysis were performed. The statistical findings were tested using two new more pollen extracts to validate the models. Three main findings of the study were (i) determination of Apigenin and Pinoresinol levels in pollen extract can give an insight into the AuNSs physical characters, (ii) the most concentrated phenolic acids and flavonoids don't need to be same to pose same dictative effect on AuNSs morphology and size, rather relatively abundant ones in the extract play the key role and (iii) differences in the polymeric structures (e. g. lignin, cellulosic compounds etc.) have minor effect on the final morphology and size of the AuNSs.
Identification of Marker Molecules in Aqueous Plant Extracts Affecting the Gold Nanostructures' Morphology and Size
(2023) Elgaleidh, M.A.M.; Tepe, H.D.; Doyuk, F.; Çeter, T.; Yazgan, I.
Hereby, we performed a comparative study using nine different aqueous pollen grain extracts from eight different genus to synthesize gold nanostructures (AuNSs) to understand if there is any possible marker that helps to predict the final morphology and size of the AuNSs. Principal component analysis (PCA) revealed that Apigenin and Pinoresinol content are the marker molecules in determination of the AuNSs physical characteristics while total protein, reducing sugar, flavonoid and phenol contents did not show any statistically meaningful outcome. We also tested the "dominancy hypothesis" by paying attention to the most concentrated phenolic acids and flavonoids in the control of AuNSs morphology and size. Three main findings of the study are (i) determination of Apigenin and Pinoresinol levels in pollen extract can give an insight into the AuNSs physical characters, (ii) the most concentrated phenolic acids and flavonoids don't need to be same to pose same dictative effect on AuNSs morphology and size, rather relatively abundant ones in the extract play the key role and (iii) differences in the polymeric structures (e.g. lignin, cellulosic compounds etc.) have minor effect on the final morphology and size of the AuNSs.
Identification of Marker Molecules in Aqueous Plant Extracts Affecting the Gold Nanostructures’ Morphology and Size
(John Wiley and Sons Inc, 2024) Elgaleidh, M.A.M.; Dilek Tepe, H.; Doyuk, F.; Çeter, T.; Yazgan, İ.
This work was performed as a comparative study using nine different aqueous pollen grain extracts from eight different genera (Juniperus, Biota, Cupressus, Abies, Pinus, Cedrus, Populus and Corylus) to synthesize gold nanostructures (AuNSs) to understand if there is any possible marker that helps to predict the final morphology and size of the AuNSs. Principal component analysis (PCA) revealed that Apigenin and Pinoresinol compounds are the marker molecules in determination of the AuNSs physical characteristics while total protein, reducing carbohydrate, flavonoid and phenol contents did not show any statistically meaningful outcome. The “dominancy hypothesis” was tested by paying attention to the most concentrated phenolic acids and flavonoids in the control of AuNSs morphology and size, for which correlation analysis were performed. The statistical findings were tested using two new more pollen extracts to validate the models. Three main findings of the study were (i) determination of Apigenin and Pinoresinol levels in pollen extract can give an insight into the AuNSs physical characters, (ii) the most concentrated phenolic acids and flavonoids don't need to be same to pose same dictative effect on AuNSs morphology and size, rather relatively abundant ones in the extract play the key role and (iii) differences in the polymeric structures (e. g. lignin, cellulosic compounds etc.) have minor effect on the final morphology and size of the AuNSs.
Palynological Investigation of Some Astragalus L. Species Distributed in Şanlıurfa
(2024) Altın, F.; Akan, H.; Çeter, T.
In this study, pollen morphologies of 5 species (Astragalus guttatus Banks & Sol., A. cretaceus Boiss., A. caprinus L., A. brachystachys DC. and A. ancistrocarpus Boiss. & Hausskn.) belonging to the Astragalus genus of the family Fabaceae, which are distributed in Şanlıurfa province, were exemined by light and electron microscope. As a result of the study, it was determined that the pollen of the taxa were monad, radial symmetrical and isopolar. Pollen of all studied species are trizonocolporate, colpus length (Clg) 16.5-27.62 µm, colpus width (Clt) 2.15-4.16 µm, pore length (Plg) 6.69-9.32 µm, pore width (Plt) was detected between 6.69-11.44 µm. Polar axis length was measured between 19.23-33.66 µm and equatorial axis diameter was measured as 14.95-28.61 µm. Ornamentation was determined as psilate, psilate-perforate, psilate-microreticulate, microreticulate in the polar region and aperture surround the while microreticulate in the equatorial region. As a result of the study, it was determined that the characters such as pollen shape, pollen size and surface ornamentation differ between species and these characters have taxonmic value in the systematic separation of species.