Browsing by Author "Çelik M."
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Scopus A comparative evaluation of hematological and biochemical parameters between the italian mullet mugil cephalus (Linnaeus 1758) and the turkish mullet chelon auratus (risso 1810)(2020-01-01) Fazio F.; Saoca C.; Acar Ü.; Tezel R.; Çelik M.; Yilmaz S.; Kesbiç O.; Yalgin F.; Yiğit M.This study aimed to carry out a comparative evaluation of the hematological profile (erythrocyte count, hemoglobin concentration, hematocrit, and Wintrobe index parameters such as mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration), in addition to certain biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, serum total protein, albumin, glucose, total cholesterol, and triglycerides), in the Italian mullet Mugil cephalus (Linnaeus 1758) and the Turkish mullet Chelon auratus (Risso 1810). Accordingly, two groups of fish were used in this study: 30 flathead grey mullets, M. cephalus, caught in Lake Faro, Italy, and 30 golden grey mullets, C. auratus, caught in the estuarine channel system of Köyceğiz–Dalyan, Turkey. Statistical evaluations (unpaired t-test) revealed that there were no significant differences in weight or total length values between the two mullet groups. However, the differences between all the evaluated blood parameters (except alanine aminotransferase) between the Italian and the Turkish mullet were significant (P < 0.0001). The present study demonstrated that there were significant hematological and biochemical differences between the Italian (M. cephalus) and the Turkish (C. auratus) mullet. Our findings also contribute to expanding the knowledge on the hematology and biochemistry of two different species of mullet originating from two different habitats. Since the evaluation of blood parameters represents an essential tool in examining the effects of environmental conditions on fish physiology and, consequently, on fish health status, further research in this field is encouraged and would be very useful.Scopus Explanation of difenoconazole removal by chitosan with Langmuir adsorption isotherm and kinetic modeling(2023-01-01) Altun Ş.; Kadak A.E.; Küçükgülmez A.; Gülnaz O.; Çelik M.In this study, the adsorption of toxic difenoconazole pesticide was investigated by using chitosan. In the first phase of the study, chitosan was extracted from deep-water pink shrimp (Parapenaeus longirostris) shells, by deacetylation of the chitin, which is separated and disposed of after meat extraction in processing facilities in Turkey. The deacetylation degree, molecular weight, viscosity, moisture, and crude-ash values of the extracted chitosan were determined. Chitosan, having a high deacetylation degree (90.21%), was used as the adsorbent. In the second phase of the study, the effects of pH, temperature, and pesticide concentration on the adsorption were investigated. The optimum pH level for pesticide adsorption was determined as 5. It was observed that the adsorption increases as the temperature increases. A rapid increase was observed within the first 5 min of the 60-minute adsorption process in difenoconazole concentrations of 5, 15, and 25 µg/L, and after 10 min, the adsorption rate was stable. The Langmuir isotherm parameters regarding the adsorption were determined as aL = 0.635, kL = 15.10, and the Qmax value was calculated as 23.77 mg/g. In the evaluation of overall study results, it was determined that the chitosan biopolymer is a suitable adsorbent for difenoconazole pesticide adsorption.Scopus Preparation and Characterization of Crayfish (Astacus leptodactylus) Chitosan with Different Deacetylation Degrees(2023-04-01) Kadak A.E.; Küçükgülmez A.; Çelik M.Background: In this study, chitosan with various deacetylation degrees was extracted from crayfish (Astacus leptodactylus) shells with the purpose of examining the effect of deacetylation on the characterization of chitosan. Objectives: Recycling of wastes has become an important issue with the advancement of shellfish processing technology. Therefore, this study examined the most important and conventional characterization parameters of chitosan extracted from crayfish shells and investigated whether crayfish chitosan can be an alternative to commercial products. Material and Methods: In order to determine the characterization of the chitosan; degree of deacetylation, yield, molecular weight, apparent viscosity, water binding capacity, fat binding capacity, moisture content, ash content, color properties, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction analyses (XRD) were applied. Results: The low (LDD) and high (HDD) deacetylated crayfish chitosan characterization results in terms of yield, molecular weight, apparent viscosity, water binding capacity, fat binding capacity, moisture content, ash content were 17.50%, 424.03-334.66 kDa, 16.82-9.63 cP, 481.29-428.04%, 419.30-355.75%, 3.32-1.03%, 0.98-1.01%, respectively. As detected by two different methods, potentiometric titration and elemental analysis, the deacetylation degrees of low and high crayfish chitosan were found close to each other, which were 76.98-94.98% and 73.79-92.06%, respectively. As the deacetylation period extended, acetyl groups were removed, and the degree of the deacetylation of crayfish chitosan increased while the apparent viscosity, molecular weight, water and fat binding capacity decreased. Conclusions: The findings of the present study are important to obtain the chitosan having various physicochemical characteristics from unevaluated crayfish wastes and to use it in many different sectors, especially biotechnology, medicine, pharmaceutical, food, and agriculture.