Browsing by Author "Çelik E.N.Y."

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Comparative bioinformatics analysis and abiotic stress responses of expansin proteins in Cucurbitaceae members: watermelon and melon
(2023-03-01) İncili Ç.Y.; Arslan B.; Çelik E.N.Y.; Ulu F.; Horuz E.; Baloglu M.C.; Çağlıyan E.; Burcu G.; Bayarslan A.U.; Altunoglu Y.C.
Watermelon and melon are members of the Cucurbitaceae family including economically significant crops in the world. The expansin protein family, which is one of the members of the cell wall, breaks down the non-covalent bonds between cell wall polysaccharides, causing pressure-dependent cell expansion. Comparative bioinformatics and molecular characterization analysis of the expansin protein family were carried out in the watermelon (Citrullus lanatus) and melon (Cucumis melo) plants in the study. Gene expression levels of expansin family members were analyzed in leaf and root tissues of watermelon and melon under ABA, drought, heat, cold, and salt stress conditions by quantitative real-time PCR analysis. After comprehensive searches, 40 expansin proteins (22 ClaEXPA, 14 ClaEXPLA, and 4 ClaEXPB) in watermelon and 43 expansin proteins (19 CmEXPA, 15 CmEXPLA, 3 CmEXPB, and 6 CmEXPLB) in melon were identified. The greatest orthologous genes were identified with soybean expansin genes for watermelon and melon. However, the latest divergence time between orthologous genes was determined with poplar expansin genes for watermelon and melon expansin genes. ClaEXPA-04, ClaEXPA-09, ClaEXPB-01, ClaEXPB-03, and ClaEXPLA-13 genes in watermelon and CmEXPA-12, CmEXPA-10, and CmEXPLA-01 genes in melon can be involved in tissue development and abiotic stress response of the plant. The current study combining bioinformatics and experimental analysis can provide a detailed characterization of the expansin superfamily which has roles in growth and reaction to the stress of the plant. The study ensures detailed data for future studies examining gene functions including the roles in plant growth and stress conditions.
Effects of Exogenous Melatonin Supplementations on Some Elemental Contents in Anatolian Black Pine (Pinus nigra J.F. Arnold. subsp. pallasiana (Lamb.) Holmboe) Seedling Tissues
(2021-08-01) Çelik E.N.Y.
A multi-directional relationship may be conceivable between elements and melatonin in sessile organisms. Melatonin is an important hormone that helps regulate metabolism. This study investigated how different doses (0 pM/control, 250 pM, 500 pM, 1000 pM, and 1500 pM) of exogenous melatonin supplementations (EMS) affected the elemental contents in Anatolian black pine (Pinus nigra Arnold. ssp. pallasiana (Lamb.) Holmboe) seedling tissues (root, stem, and needle). Two different application forms (root-dipping and needle-spraying) were selected in the study. In the samples of seedling tissues, sodium (Na), potassium (K), calcium (Ca), iron (Fe), aluminum (Al), magnesium (Mg)/ppm; chrome (Cr), cobalt (Co), copper (Cu), manganese (Mn), nickel (Ni), phosphorus (P), selenium (Se), silicium (Si), silver (Ag), sulfur (S), zinc (Zn), and molybdenum (Mo)/ppb were analyzed using inductively coupled plasma optical emission spectroscopy (ICP-OES). Of the 18 elements examined, there was a statistically significant difference (p<0.05) between all seedling tissues and different doses of EMS. The results show that EMS may have the regulatory effect on seedling tissue element metabolism.
Effects of Exogenous Melatonin Supplementations on Some Elemental Contents in Anatolian Black Pine (Pinus nigra J.F. Arnold. subsp. pallasiana (Lamb.) Holmboe) Seedling Tissues
(2021-08-01) Çelik E.N.Y.; Celik, ENY
A multi-directional relationship may be conceivable between elements and melatonin in sessile organisms. Melatonin is an important hormone that helps regulate metabolism. This study investigated how different doses (0 pM/control, 250 pM, 500 pM, 1000 pM, and 1500 pM) of exogenous melatonin supplementations (EMS) affected the elemental contents in Anatolian black pine (Pinus nigra Arnold. ssp. pallasiana (Lamb.) Holmboe) seedling tissues (root, stem, and needle). Two different application forms (root-dipping and needle-spraying) were selected in the study. In the samples of seedling tissues, sodium (Na), potassium (K), calcium (Ca), iron (Fe), aluminum (Al), magnesium (Mg)/ppm; chrome (Cr), cobalt (Co), copper (Cu), manganese (Mn), nickel (Ni), phosphorus (P), selenium (Se), silicium (Si), silver (Ag), sulfur (S), zinc (Zn), and molybdenum (Mo)/ppb were analyzed using inductively coupled plasma optical emission spectroscopy (ICP-OES). Of the 18 elements examined, there was a statistically significant difference (p<0.05) between all seedling tissues and different doses of EMS. The results show that EMS may have the regulatory effect on seedling tissue element metabolism.
Effects of Melatonin Applications on Anatolian Black Pine (Pinus nigra J. F. Arnold. subsp. pallasiana (Lamb.) Holmboe) Afforestation Performance in Semi-Arid Areas
(2023-01-01) Çelik E.N.Y.; Ayan S.; Özel H.B.; Turfan N.; Yer B.M.; Abdaloğlu G.
Melatonin, a substantial hormone, is a natural antioxidant agent that functions as a protector against the harmful effects of free radicals. Studies have found that "exogenous melatonin" applications have a positive effect on the growth and development of plants. This study investigated the adaptation of the seedlings that were transported from the nursery to the afforestation site for the process of planting. In 2019 the 2+0 aged bare-rooted Kastamonu/Taşköprü Anatolian Black pine seedlings, which are suitable for planting in semi-arid areas, were selected as research materials. Four different doses of “exogenous melatonin" (250, 500, 1000, and 1500 μM) were administered through two different methods (root-dipping and needle-spraying). Morphological seedling characteristics and bioactive chemical variables were measured for the control group and the seedlings treated with different doses of melatonin. Antioxidant enzyme activities were identified. When both the needle-spraying and root-dipping methods for melatonin application were evaluated in terms of morphological and biochemical variables, the best results were determined in low doses (250 to 500 μM). The results suggest that melatonin provides support to the metabolic process for the resistance of seedlings to low temperatures and semi-arid climatic conditions.
Effects of Nanoparticle Applications on Seedling Survival and Morphological Characteristics in Scots Pine Afforestation
(North Carolina State University, 2023) Ayan S.; Çelik E.N.Y.; Gülseven O.; Yer B.M.; Eskiömer M.
This study was conducted in the afforestation area, using bare-root 2+0-year-old Scots pine seedlings from Kastamonu. The study aimed to determine the impact of nanoparticle (NP) applications on seedling morphological characteristics and seedling survival success. Three different concentrations (low, medium, high) and four different nanoparticle types [Fe₃O₄, CuO, ZnO, TiO2] were applied to the plant root-dipping method in the study. The effects of NP treatments on seedling height (SH), root collar diameter (RCD), stem fresh weight (SFW), root new weight (RFW), seedling fresh weight (SEFW), root dry weight (RDW), stem dry weight (SDW), seedling dry weight (SEDW), sturdiness quotient (SI), root: shoot ratio (R/S), and seedling survival in the field were evaluated. The study results revealed that NP types significantly affected all seedling variables except RFW, SDW, RDW, and SEDW, and NP doses significantly affected all seedling variables except RFW. The binary interaction effects of NP types and doses had a significant effect on all seedling variables, and higher values were obtained compared to the control treatment. Medium and high NP doses were more effective in seedling growth than low doses; the percentage of seedling survival was 61.4% in the control treatment and 95% in the TiO2-Medium NP treatment combination.
The influence of effective microorganisms on physiological characteristics of containerized taurus cedar (Cedrus libani A. Rich.) seedlings
(2022-01-01) Ayan S.; Çalışkan E.; Özel H.B.; Çelik E.N.Y.; Yılmaz E.; Gülseven O.; Akın Ş.S.
Background: In this study, the impact of Effective Microorganism (EM) applications on some physiological characteristics of 2+0 aged, containerized Taurus cedar (Cedrus libani A.Rich.) seedlings were investigated. EM-1, EM-A, EM-5, and EM-Gold were used as effective microorganism varieties. EM application was repeated twice at two different times. The first application was carried out in April-May 2017 at the beginning of the vegetation period, and the second application was carried out in June-July, when the growth of seedlings was the most active period, according to a randomized plot design with three replications. Results: Physiological measurements on seedlings were carried out at the end of the second vegetation period. According to the results of the study, it was determined that the EM type, dose and application time factors had a significant effect on physiological characteristics such as Chlorophyll a, Chlorophyll b, Total Chlorophyll, Photosynthesis Rate, Transpiration and Relative Humidity. EM-A had a positive effect on photosynthesis rate and EM-5 had a positive effect on all other measured physiological parameters. High dose of EMs had the most positive effect on relative humidity, while medium dose of EMs had the most positive effect on chlorophyll values, transpiration rate and photosynthesis rate. In addition, it was determined that EM application at the beginning of the vegetation period had an increasing effect on all measured physiological parameter values. Conclusion: According to the results of the study, it was concluded that EMs have a positive effect on the physiological quality characteristics of Taurus cedar seedlings and can contribute to the production of seedlings with higher adaptability in afforestation.