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Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes

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dc.contributor.authorBulut, Oya
dc.contributor.authorUyunmaz Saklı, Gülşah
dc.contributor.authorHasöksüz, Mustafa
dc.contributor.authorDik, Irmak
dc.contributor.authorHadimli, Hasan Hüseyin
dc.contributor.authorHitit, Mustafa
dc.date.accessioned2026-01-04T14:31:00Z
dc.date.issued2020-09-01
dc.description.abstractAim: Coronaviruses and Rotaviruses are important virologic factors for both animal and human health in Turkey and the world Bovine Rotavirus (BRV) and Bovine Coronavirus (BCoV) in cattle cause significant economic losses The aim of this study was to determine the presence of BRV and BCoV in Anatolian buffaloes which were on the same farms with cattle For this purpose, presence of these two viruses were investigated by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and BRV-BCoV Rapid tests and sensitivity and specificity ratios of these two tests were compared Materials and Methods: In this study, 230 Anatolian buffaloes were clinically evaluated in cattle farms in Afyonkarahisar region Fecal samples were collected from 27 buffaloes which had clinical signs (weakness, dehydration, vomiting, watery consistency and yellow stool) The fecal samples were evaluated by Rapid Test and RT-PCR for Bovine Rotavirus and Bovine Coronavirus The analyzes were performed according to the procedure of the commercial RT-PCR and rapid kits
dc.description.urihttps://doi.org/10.15312/eurasianjvetsci.2020.273
dc.description.urihttps://dx.doi.org/10.15312/eurasianjvetsci.2020.273
dc.description.urihttps://search.trdizin.gov.tr/yayin/detay/409512
dc.description.urihttps://doi.org/https://doi.org10.15312/eurasianjvetsci.2020.273
dc.description.urihttps://hdl.handle.net/20.500.12831/13271
dc.description.urihttps://hdl.handle.net/20.500.12395/43288
dc.identifier.doi10.15312/eurasianjvetsci.2020.273
dc.identifier.endpage165
dc.identifier.issn2146-1953
dc.identifier.openairedoi_dedup___::bafe40a7cd9fbf548fb904b4830a42b0
dc.identifier.startpage159
dc.identifier.urihttps://hdl.handle.net/20.500.12597/38229
dc.identifier.volume36
dc.language.isoeng
dc.publisherSelçuk University Press
dc.relation.ispartofEurasian Journal of Veterinary Sciences
dc.rightsOPEN
dc.subjectBCoV
dc.subjectRapid test
dc.subjectBovine coronavirus
dc.subjectRT-PCR
dc.subjectAnatolian water buffaloes
dc.subjectBRV
dc.subjectAnadolu mandas
dc.subjectBovine rotavirus
dc.subjectHızlı test
dc.subject.sdg2. Zero hunger
dc.subject.sdg3. Good health
dc.titleComparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloes
dc.typeArticle
dspace.entity.typePublication
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