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Bienzymatic fluorescence detection based on paraoxonase and laccase on anthracene-sequestered polyamic acid films: A novel approach for inhibition-based sensors

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dc.contributor.authorEsen, Elif
dc.contributor.authorYazgan, İdris
dc.contributor.authorOdaci Demirkol, Dilek
dc.date.accessioned2026-01-04T14:43:33Z
dc.date.issued2020-12-01
dc.description.abstractAbstract A fluorescence assay based on paraoxonase (PO) and laccase (Lac) immobilized on anthracene-sequestered polyamic acid films is now being reported for the first time for ciprofloxacin (CFx) detection. In enzymatic reaction, PO converts phenyl acetate (PA) to acetic acid and phenol. The formed phenol is further oxidized by laccase using oxygen as a co-substrate. For indirectly fluorescence measurements of PA hydrolysis, increased fluorescence intensity was measured after oxygen consumption by laccase. This is because oxygen is quencher of anthracene (Ant) incorporated in the sequestered poly(amic) acid (PAA) film. The detection mechanism was based on inhibition of PO activity by CFx. Using PAA provided the advantage of controlling the film thickness. Firstly, morphology of PAA-Ant polymeric film was characterized by scanning electron microcopy (SEM) and the success of PO-Lac immobilization on PAA-Ant was proven by SEM plus Energy-dispersive X-ray spectroscopy (SEM-EDX) and fluorescence measurements. Then Ant-PAA/PO/Lac is calibrated for PA and CFx without any interfering of some potential interferences. All results make it a promising tool for monitoring CFx at minute levels in samples.
dc.description.urihttps://doi.org/10.1016/j.mtcomm.2020.101672
dc.description.urihttps://dx.doi.org/10.1016/j.mtcomm.2020.101672
dc.description.urihttps://hdl.handle.net/11454/61512
dc.description.urihttps://hdl.handle.net/11454/70189
dc.identifier.doi10.1016/j.mtcomm.2020.101672
dc.identifier.issn2352-4928
dc.identifier.openairedoi_dedup___::ee804268513ee0c0e923a8fbf8cc8b7f
dc.identifier.orcid0000-0002-0264-1253
dc.identifier.orcid0000-0002-7954-1381
dc.identifier.scopus2-s2.0-85091255395
dc.identifier.startpage101672
dc.identifier.urihttps://hdl.handle.net/20.500.12597/38372
dc.identifier.volume25
dc.identifier.wos000601000200003
dc.language.isoeng
dc.publisherElsevier BV
dc.relation.ispartofMaterials Today Communications
dc.rightsCLOSED
dc.subjectAntibiotic detection
dc.subjectInhibition-based sensing
dc.subjectParaoxonase
dc.subjectLaccase
dc.titleBienzymatic fluorescence detection based on paraoxonase and laccase on anthracene-sequestered polyamic acid films: A novel approach for inhibition-based sensors
dc.typeArticle
dspace.entity.typePublication
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