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LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of Boerhavia diffusa Extracts: A Potential Raw Material for Functional Applications

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dc.contributor.authorSinan, Kouadio Ibrahime
dc.contributor.authorAkpulat, Uğur
dc.contributor.authorAldahish, Afaf A.
dc.contributor.authorCelik Altunoglu, Yasemin
dc.contributor.authorBaloğlu, Mehmet Cengiz
dc.contributor.authorZheleva-Dimitrova, Dimitrina
dc.contributor.authorGevrenova, Reneta
dc.contributor.authorLobine, Devina
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.contributor.authorEtienne, Ouattara Katinan
dc.contributor.authorZengin, Gokhan
dc.contributor.authorMahmud, Shafi
dc.contributor.authorCapasso, Raffaele
dc.date.accessioned2026-01-04T16:02:26Z
dc.date.issued2021-12-16
dc.description.abstractBoerhavia diffusa is a great tropical plant and is widely used for various traditional purposes. In the present study, we examined the influence of solvents (dichloromethane, ethyl acetate, methanol and infusion (water)) on chemical composition and biological capabilities of B. diffusa. An UHPLC-HRMS method was used to determine the chemical characterization. The biological ability was examined for antioxidant, enzyme inhibitory and anti-cancer effects. To evaluate antioxidant effects, different chemical methods (ABTS, DPPH, CUPRAC, FRAP, metal chelating and phosphomolybdenum) were applied. With regard to enzyme inhibitory properties, cholinesterases, amylase, glucosidase and tyrosinase were used. The MDA-MB-231 breast cancer cell line was chosen to determine anticancer activity. Based on the UHPLC-HRMS analysis, 37 specialized metabolites were dereplicated and identified in the studied extracts. Results revealed the presence of 15 hydroxybenzoic, hydroxycinnamic, acylquinic acids, and their glycosides, one rotenoid, seven flavonoids, 12 fatty acids and two other glycosides. Among the tested extracts, the methanol extract showed a stronger antioxidant ability compared with other extracts. The methanol extract also showed the best inhibitory effects on tyrosinase and glucosidase. In the anti-cancer evaluation, the methanol extract showed stronger anticancer effects compared with water extract. In summary, our observations can contribute to the establishment of B. diffusa as a potential candidate for functional applications in the preparation.
dc.description.urihttps://doi.org/10.3390/antiox10122003
dc.description.urihttps://www.mdpi.com/2076-3921/10/12/2003/pdf
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/34943106
dc.description.urihttp://dx.doi.org/10.3390/antiox10122003
dc.description.urihttps://doaj.org/article/dce6d9ef817343ee89dbaddbfdf70664
dc.description.urihttp://hdl.handle.net/11588/865857
dc.description.urihttps://dx.doi.org/10.3390/antiox10122003
dc.description.urihttps://hdl.handle.net/11588/865857
dc.identifier.doi10.3390/antiox10122003
dc.identifier.eissn2076-3921
dc.identifier.openairedoi_dedup___::3cfc417f7ae3bf7f79f38bb5bf71a6be
dc.identifier.orcid0000-0001-8126-8209
dc.identifier.orcid0000-0002-5073-2088
dc.identifier.orcid0000-0002-1952-9903
dc.identifier.orcid0000-0002-1254-2419
dc.identifier.orcid0000-0002-6398-0371
dc.identifier.orcid0000-0003-3962-8666
dc.identifier.orcid0000-0001-6548-7823
dc.identifier.orcid0000-0002-3335-1822
dc.identifier.pubmed34943106
dc.identifier.scopus2-s2.0-85121147814
dc.identifier.startpage2003
dc.identifier.urihttps://hdl.handle.net/20.500.12597/39232
dc.identifier.volume10
dc.identifier.wos000735491800001
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofAntioxidants
dc.rightsOPEN
dc.subjectAnti-cancer
dc.subjectAntioxidant
dc.subjectBoerhavia diffusa
dc.subjectEnzyme inhibition
dc.subjectFlavonoids
dc.subjectFunctional applications
dc.subjectFlavonoids
dc.subjectantioxidant
dc.subjectAnti-cancer
dc.subjectFunctional applications
dc.subject<i>Boerhavia diffusa</i>
dc.subjectRM1-950
dc.subjectArticle
dc.subjectBoerhavia diffusa
dc.subjectEnzyme inhibition
dc.subjectfunctional applications
dc.subjectflavonoids
dc.subjectTherapeutics. Pharmacology
dc.subjectAntioxidant
dc.subjectenzyme inhibition
dc.subjectanti-cancer
dc.subject<i>Boerhavia diffusa</i>
dc.subjectantioxidant
dc.subjectenzyme inhibition
dc.subjectanti-cancer
dc.subjectflavonoids
dc.subjectfunctional applications
dc.subject.sdg3. Good health
dc.titleLC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of Boerhavia diffusa Extracts: A Potential Raw Material for Functional Applications
dc.typeArticle
dspace.entity.typePublication
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