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Detection of bovine respiratory syncytial virus, Pasteurella multocida, and Mannheimia haemolytica by immunohistochemical method in naturally-infected cattle

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dc.contributor.authorYaman, Turan
dc.contributor.authorBüyükbayram, Hüseyin
dc.contributor.authorÖzyıldız, Zafer
dc.contributor.authorTerzi, Funda
dc.contributor.authorUyar, Ahmet
dc.contributor.authorKeleş, Ömer Faruk
dc.contributor.authorÖzsoy, Şule Yurdagül
dc.contributor.authorYener, Zabit
dc.date.accessioned2026-01-04T12:27:30Z
dc.date.issued2018-12-01
dc.description.abstractAbstract Introduction: The aim of this study was to determine the predisposing effect of bovine respiratory syncytial virus (BRSV) on Pasteurella spp. infection in naturally-induced pneumonia in cattle by immunohistochemical labelling. Material and Methods: Lungs of cattle slaughtered in the slaughterhouse were examined macroscopically, and 100 pneumonic samples were taken. The samples were fixed in 10% neutral formalin and embedded in paraffin by routine methods. Sections 5 μm in thickness were cut. The streptavidin-peroxidase method (ABC) was used to stain the sections for immuno-histochemical examination. Results: BRSV antigens were found in the cytoplasm of epithelial cells of bronchi, bronchioles, and alveoles and within inflammatory cell debris and inflammatory exudate in bronchial lumens. Pasteurella spp. antigens were detected in the cytoplasm of the epithelial cells of bronchi and bronchioles, and in cells in the lumens of bronchi and bronchioles. Eleven cases were positive for only one pathogen (six for BRSV and five for Pasteurella spp.), while 35 cases were positive for 2 pathogens: BRSV plus P. multocida (n = 21) or M. haemolytica (n = 14). Conclusion: The presence of high levels of BRSV in dual infections indicates that BSRV may be the main pneumonia-inducing agent and an important predisposing factor for the formation of Pasteurella spp. infections in cattle naturally afflicted with pneumonia.
dc.description.urihttps://doi.org/10.2478/jvetres-2018-0070
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/30729200
dc.description.urihttp://dx.doi.org/10.2478/jvetres-2018-0070
dc.description.urihttps://doaj.org/article/6203538e3f0d4c2d95ab4561acf4ac1e
dc.description.urihttps://dx.doi.org/10.2478/jvetres-2018-0070
dc.description.urihttps://avesis.yyu.edu.tr/publication/details/026df9e5-709a-4319-bf6e-a17c43705bb0/oai
dc.description.urihttps://sciendo.com/article/10.2478/jvetres-2018-0070
dc.description.urihttps://hdl.handle.net/11468/13672
dc.description.urihttps://hdl.handle.net/20.500.12483/8627
dc.identifier.doi10.2478/jvetres-2018-0070
dc.identifier.eissn2450-8608
dc.identifier.endpage445
dc.identifier.openairedoi_dedup___::bdeaaad39788795f908164fb8695aa6d
dc.identifier.orcid0000-0002-6365-5843
dc.identifier.pubmed30729200
dc.identifier.scopus2-s2.0-85063346817
dc.identifier.startpage439
dc.identifier.urihttps://hdl.handle.net/20.500.12597/37059
dc.identifier.volume62
dc.identifier.wos000455210500006
dc.language.isoeng
dc.publisherWalter de Gruyter GmbH
dc.relation.ispartofJournal of Veterinary Research
dc.rightsOPEN
dc.subjectm. haemolytica
dc.subjectVeterinary medicine
dc.subjectImmunohistochemistry
dc.subjectP. multocida
dc.subjectBovine respiratory syncytial virus
dc.subjectp. multocida
dc.subjectcattle
dc.subjectbovine respiratory syncytial virus
dc.subjectimmunohistochemistry
dc.subjectSF600-1100
dc.subjectCattle
dc.subjectM. haemolytica
dc.subjectResearch Article
dc.subject.sdg2. Zero hunger
dc.subject.sdg3. Good health
dc.titleDetection of bovine respiratory syncytial virus, Pasteurella multocida, and Mannheimia haemolytica by immunohistochemical method in naturally-infected cattle
dc.typeArticle
dspace.entity.typePublication
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The samples were fixed in 10% neutral formalin and embedded in paraffin by routine methods. Sections 5 μm in thickness were cut. The streptavidin-peroxidase method (ABC) was used to stain the sections for immuno-histochemical examination.</jats:p> <jats:p> <jats:bold>Results:</jats:bold> BRSV antigens were found in the cytoplasm of epithelial cells of bronchi, bronchioles, and alveoles and within inflammatory cell debris and inflammatory exudate in bronchial lumens. <jats:italic>Pasteurella</jats:italic> spp. antigens were detected in the cytoplasm of the epithelial cells of bronchi and bronchioles, and in cells in the lumens of bronchi and bronchioles. 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