Harmankaya, A.Çınar, İ.Yayla, M.Harmankaya, S.Beytur, M.Öziç, C.2024-05-172024-05-172024Harmankaya, A., Çinar, İ., Yayla, M., Harmankaya, S., Beytur, M., Öziç, C. (2024). In vitro Evaluation of the Effects of Inula viscosa’s Different Extracts on Wound Healing and Oxidative Stress in Mouse L929 Fibroblast Cell Line. FABAD Journal of Pharmaceutical Sciences, 49(1), 129-1421300-4182https://search.trdizin.gov.tr/publication/detail/1229690/in-vitro-evaluation-of-the-effects-of-inula-viscosas-different-extracts-on-wound-healing-and-oxidative-stress-in-mouse-l929-fibroblast-cell-linehttps://hdl.handle.net/20.500.12597/33151This study evaluated the effects of extracts prepared using two different methods (decoction extraction and soxhlet ethyl acetate/hexane extraction) from Inula viscosa on H2O2-induced oxidative stress and wound healing model in mouse L929 fibroblast cells. The cytotoxic effect started to disappear statistically (p<0.05) at concentrations of Soxhlet ethyl acetate/hexane extracts (SoxEHE) in and below 0.1 mg/ mL, while the same effect was observed at concentrations of decoction extracts (DE) in and below 0.2 mg/mL. Therefore, 0.2 and 0.02 mg/ mL concentrations of DE, and 0.1 and 0.01 mg/mL concentrations of SoxEHEs were used. While cell migration was positively affected in all research concentrations, statistically significant results (p<0.05) were obtained from 0.2 mg/mL of DE and 0.1 mg/mL of SoxEHE extracts. Malondialdehyde (MDA) levels were found to be statistically (p <0.05) decreased, but COL1A1 levels were higher in cell lines treated with oxidative stress + extract than in the cell line treated only with H2O2, and reduced glutathione (GSH) levels were higher in cell lines only treated only with extract than in oxidative stress-induced cell lines. Consequently, it has been observed that the extracts have positive effects on migration and oxidative stress. T herefore, I. viscosa may serve as a new therapeutic agent for wound healing.enginfo:eu-repo/semantics/openAccessMedicinal plants, extraction, scratch assay, cell proliferation, oxidative statusarticle10.55262/fabadeczacilik.136402912296901291424912651-4648