Cincin Z., Unlu M., Kiran B., Bireller E., Baran Y., Cakmakoglu B.Cincin, ZB, Unlu, M, Kiran, B, Bireller, ES, Baran, Y, Cakmakoglu, B2023-05-092023-05-092015-04-282015.01.011219-4956https://hdl.handle.net/20.500.12597/12679Quercetin, which is the most abundant bioflavonoid compound, is mainly present in the glycoside form of quercitrin. Although different studies indicated that quercitrin is a potent antioxidant, the action of this compound is not well understood. In this study, we investigated whether quercitrin has apoptotic and antiproliferative effects in DLD-1 colon cancer cell lines. Time and dose dependent antiproliferative and apoptotic effects of quercitrin were subsequently determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, detection of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential (MMP) and also the localization of phosphatidylserine (PS) in the plasma membrane. There were significant increases in caspase-3 activity, loss of MMP, and increases in the apoptotic cell population in response to quercitrin in DLD-1 colon cancer cells in a time- and dose-dependent manner. These results revealed that quercitrin has antiproliferative and apoptotic effects on colon cancer cells. Quercitrin activity supported with in vivo analyses could be a biomarker candicate for early colorectal carcinoma.falseAntiproliferative | Apoptosis | Colon Cancer | QuercitrinArticle10.1007/s12253-014-9825-310.1007/s12253-014-9825-32-s2.0-84938214197WOS:000354265700013333338211532-2807