Cevahir, F.Düzlü, Ö.Atelge, M.Yildirim, A.2024-11-052024-11-052024.01.011300-0861https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=dspace_ku&SrcAuth=WosAPI&KeyUT=WOS:001334949700001&DestLinkType=FullRecord&DestApp=WOS_CPLhttps://hdl.handle.net/20.500.12597/33708This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I (mt-COI), mt-COII, and internal transcribed spacer-2 (ITS-2) gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism (RFLP) was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species (n=220) collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica (n=105), Blatta orientalis (n=86), and Periplaneta americana (n=29). A total of 13 haplotypes were detected and maximum likelihood (ML) analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 (79.3%) belonged to helminth species and 12 (20.7%) to protozoan species. The results showed that B. germanica (58.6%) had the highest prevalence, followed by Bl. orientalis (32.8%) and P. americana (8.6%). The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.eninfo:eu-repo/semantics/openAccessCockroachDNA barcodingITS-2mt-COI and mt-COIIVectorArticle10.33988/auvfd.13001940013349497000014174267141308-2817