Bayram, PinarAksak Karamese, SelinaOzdemır, BengulSalum, CagatayErol, Huseyin SerkanKaramese, Murat2023-04-062023-04-062023-03-291532-2513https://hdl.handle.net/20.500.12597/3220In this study, our aim was to investigate the possible protective and therapeutic effects of these two flavonoids, baicalein and naringin, in 50 and 100 mg/kg doses applied both before and after cecal ligation and puncture (CLP) procedures in a polymicrobial sepsis rat model, and evaluate possible contribution of oxidative and inflammatory markers by immunological, biochemical, molecular, and histopathological methods.Sixty-six Wistar albino rats were divided into 11 groups. The pro-inflammatory (TNF-alpha, IL-1-beta, and IL-6) and anti-inflammatory (TGF-beta and IL-10) cytokine levels were measured by ELISA technique. CD3, CD68, and nuclear factor kappa B positivity rates were detected by immunohistochemical methods. Oxidative stress parameters (MDA, SOD, and GSH) were measured by tissue biochemistry.Sepsis caused a significant increase in all pro-inflammatory cytokine levels and MDA activity. Also, it led to an increase in the positivities of CD3, CD68, and NF-κB markers. However, especially pre-CLP doses of baicalein and naringin inhibited the inflammation process by suppressing pro-inflammatory and increasing anti-inflammatory cytokine levels, as well as regulating oxidative stress process by normalizing the oxidant/anti-oxidant enzyme levels.Both pre- and post-application of baicalein and naringin are quite effective to prevent sepsis-caused cellular processes. This protective and therapeutic effects by baicalein and naringin in animals with sepsis seems to be originated from the high antioxidant capacity and inhibition of pro-inflammatory cytokine production. Thus, those natural agents may prove to be a valuable protective agent against septic shock.enBaicaleinCLPFlavonoidsIL-6InflammationNaringinOxidative stressSepsisTNF-alphaJournal Article10.1080/08923973.2023.219714336988563