TATAR, M.2023-11-072023-11-072023Tatar, M. (2023). The Effect of N-Acetylcysteine Use on Endoplasmic Reticulum Stress in the Kidney Tissues of Obese Rats. Kocatepe Veterinary Journal, 16(3), 301-3091308-1594https://search.trdizin.gov.tr/publication/detail/1204596/the-effect-of-n-acetylcysteine-use-on-endoplasmic-reticulum-stress-in-the-kidney-tissues-of-obese-ratshttps://hdl.handle.net/20.500.12597/17848Endoplasmic reticulum (ER) stress has recently acquired increasing importance in the pathogenesis of obesity-associated kidney disease. N-acetylcysteine, otherwise known as NAC, is an antioxidant that works directly and indirectly by increasing the production of antioxidants in cells. A diet consisting of 60% calories from fat was used to establish the obesity model for the present investigation. In the NAC and obesity + NAC (ObNAC) groups, NAC was administered by intragastric tube at 150 mg/kg for eight weeks. GRP78 and PERK expressions were determined immunohistochemically in sections collected from kidney tissues at the end of the experiment. The GRP78 H score was significantly higher in the obese group than in the control, NAC, and ObNAC groups (p<0.01). The ObNAC group H-score was significantly lower than that of the obese group (p < 0.01) but was not different from the control and NAC groups. The obese group PERK H-score was also significantly higher than the control, NAC, and ObNAC groups (p<0.01). In the ObNAC group, the H-score was significantly lower than that in the obese group (p<0.01) and significantly higher than those in the control and NAC groups (p<0.01). Increasing changes in stress markers may be improved by NAC application, since obesity induced by a high-fat diet activates ER stress in kidney tissue.enginfo:eu-repo/semantics/openAccessER stress, GRP78 N-acetylcysteine ,Obesity, PERKarticle10.30607/kvj.131284712045963013091632147-68530000-0002-5707-8832787675