Ozgur CELEBIIrfan CINARSerkan YILDIRIMKagan Tolga CINISLIDemet CELEBIIclal HALICIZekai HALICINurullah AKGUNHalil KESKIN2023-04-132023-04-132022-02-01Bozlak, Ö., Cinar, I., Yildirim, S., Cinisli, K., Çelebi̇, D., Halici, I., Halici, Z., Akgun, N., Keski̇n, H. (2022). Effect of Tocilizumab on Acinetobacter baumannii Lung Infection in an Immunosuppressed Rat Model. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(1), 87-961300-6045https://search.trdizin.gov.tr/publication/detail/517691/effect-of-tocilizumab-on-acinetobacter-baumannii-lung-infection-in-an-immunosuppressed-rat-modelhttps://hdl.handle.net/20.500.12597/6336Our study aimed to investigate effect of tocilizumab on the lung tissue in the presence of Acinetobacter baumannii infection in immunosuppressed rats. A forty-eight female Wistar albino rats were divided equally into eight groups: Group 1: Healthy (H), Group 2: Immunosuppressed (IM), Group 3: Healthy rats given A. baumannii bacteria (H+BAC), Group 4: Immunosuppressed rats given A. baumannii bacteria (IM+BAC), Group 5: Healthy rats given tocilizumab (H+TCZ), Group 6: Immunosuppressed rats given tocilizumab (IM+TCZ), Group 7: Healthy rats given A. baumannii bacteria and tocilizumab (H+BAC+TCZ), Group 8: Immunosuppressed rats given tocilizumab and A. baumannii bacteria (IM+BAC+TCZ). Fourteen days after the immunosuppression of group 2, 4, 6 and 8 with hydrocortisone, group 3, 4, 7 and 8 were A. baumannii was dropped into the trachea. One hour after A. baumannii application, TCZ was administered to Groups 5, 6, 7 and 8. NF-κB, IL-6 and NLRP3 mRNA expressions were decreased in the IM group compared to the healthy group (P<0.05). Although NF-κB, IL-6 and NLRP3 mRNA expression decreased in the IM+TCZ group compared to the healthy group (P<0.05) NF-κB, IL-6 and NLRP3 mRNA expression increased in the H+TCZ group (P<0.05). Despite decreasing cytokines, A. baumannii has been shown to increase infection-related lung injury. This suggests that in patients currently or recently using steroids, tocilizumab may increase organ damage due to opportunistic infection.enginfo:eu-repo/semantics/openAccessRESEARCH10.9775/kvfd.2021.2649151769187962811309-2251