Büşra DİNÇERAzat ÇEKDAR GÜNDOĞMUŞİrfan ÇINAR2023-07-212023-07-212023-07-01Di̇nçer, B., Gündoğmuş, A., Çinar, İ. (2023). Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. Experimental Biomedical Research, 6(3), 238-248https://search.trdizin.gov.tr/publication/detail/1188076/jaceosidin-protects-l929-fibroblast-cells-by-down-regulation-of-proinflammatory-cytokines-and-attenuation-of-oxidative-stress-induced-impairment-of-cell-proliferation-and-migrationhttps://hdl.handle.net/20.500.12597/16175Aim: Oxidative stress has been a significant factor in wound-healing pathophysiology for a long time. Antioxidants, especially natural compounds, have recently been emphasized in instructions for wound healing treatments. Jaceosidin (JACE), a flavone derived from Artemisia princeps, is a potent antioxidant. This study aims to investigate JACE’s anti-inflammatory and antioxidant properties and its capacity to improve the effects of in vitro wound healing. Methods: Wound healing activities have been tested using cell proliferation and migration in vitro assays in the mouse fibroblast cell line L929. The concentration of hydrogen peroxide (H2O2-0.5 mM) has been used to induce the oxidative stress model. Tumor necrosis factor-alpha (TNF-α) and nuclear factor (NF-) have been investigated as inflammatory indicators. Antioxidant activity has been checked using total antioxidant status (TAS) and total oxidant status (TOS) tests. Results: JACE has significantly increased the proliferation of fibroblasts dose-dependent manner. It has enhanced the cell migration rate of fibroblasts compared with the H2O2 group. JACE at a concentration of 50 and 100 μM has significantly decreased TOS and oxidative stress index (OSI) levels and increased TAS levels. The anti-inflammatory mechanism of JACE has involved down-regulation of the mRNA expressions of the NF- and TNF-α in a dose-dependent manner. Conclusions: JACE has beneficial impacts on fibroblast viability and migration qualities through antioxidative actions and down-regulating proinflammatory cytokines through anti-inflammatory effects to promote wound healing. The present study shows that JACE may help to increase the range of available treatments for woundhealing by reducing inflammation and oxidative stress.enginfo:eu-repo/semantics/openAccessRESEARCH10.30714/j-ebr.2023.1881188076238248632618-6454