Başkan C.Sırıken B.Tüfekci E.F.Kılınç Ç.Ertürk Ö.Erol İ.2023-04-112023-04-122023-04-112023-04-122022-08-013028933https://hdl.handle.net/20.500.12597/4070Carbapenems are the most effective agents for treating clinical P. aeruginosa (PsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence genes, biofilm formation and a class 1 (Cls1) integron. A total of 52 carbapenem-resistant PsA (CRPsA) isolates obtained from Kastamonu, Turkey was analyzed. For the isolation and identification of CRPsA isolates, a conventional culture method, an automated VITEK-2 compact system, and oprL gene-based molecular technique were applied. The two QS system genes were detected in 51 (98.1%), and co-existed of four two QS system genes (lasI/R and rhIl/R genes) were determined in 41 (78.8%) of the isolates. algD, lasB, toxA and aprA genes were detected in between 46.1 and 88.5%, and co-existence of four two QS system genes with four virulence genes were detected in 40.4% of the isolates. Biofilm formation using microtiter plate assay and slime production using Congo Red Agar and Cls1 integron were determined in 84.6%, 67.3% and 51.9% of the isolates, respectively. According to statistical analyses results, there was a significant positive correlation (p <.10) between the las and the rhl systems and a strongly and positive correlation (p <.01 or p <.05) between the rhl system—three virulence genes and slime production—and among some virulence genes. In conclusion, the CRPsA isolates tested in the study are highly virulent and QS systems have a significant role in pathogenesis.falseBiofilm | Carbapenem-resistant Pseudomonas aeruginosa | Class 1 integron | QS system | Virulence genesArticle10.1007/s00203-022-03061-y2-s2.0-8513372966535802194