Tümer, Kenan C, Kılınç, Mehmet ATümer K.C., Kılınç M.A.Tumer, KC, Kilinc, MA2023-05-082023-05-082023-03-012023-01-012023.01.010275-6382https://hdl.handle.net/20.500.12597/11725Accurate and rapid measurement of blood β-hydroxybutyrate (β-OHB) concentrations is critical to identify hyperketonemia in sheep.The objective of this study was to test the performance of Freestyle Optium Neo H and TaiDoc-TD4235 point-of-care (POC) devices for measuring blood β-OHB concentrations in sheep.Venous blood samples from 105 sheep were evaluated with Freestyle Optium Neo H and TaiDoc-TD4235 meters. A reference approach was the laboratory measurement of serum BHB concentrations using the Randox D-3 Hydroxybutyrate reagent kit. Data were analyzed using Passing- Bablok regression and Bland-Altman analysis. The diagnostic accuracy was assessed using serum β-OHB concentration at a cut-off value of 0.8 mmol/L.Passing-Bablok regression analysis revealed an intercept of 0.066 (CI : 0.018-0.125) and slope of 1.242 (CI : 1.136-1.342) for Freestyle Optium Neo H. Intercept and slope values for the TaiDoc-TD4235 meter were 0.625 (CI : 0.539-0.724) and 1.265 (CI : 1.044-1.497), respectively. The Bland-Altman plot revealed a bias of 0.224 mmol/L and 0.737 mmol/L for Freestyle Optium Neo H and TaiDoc-TD4235 meters, respectively, compared with the reference method. Observed Total Error (TE ) was 56.7% for the Freestyle Optium Neo H and 168.7% for the Taidoc-TD4235. The sensitivity and specificity for the Freestyle Optium Neo H were 100% and 80.9%, respectively, at the threshold of 0.8 mmol/L. The Taidoc-TD4235 demonstrated a sensitivity of 93.3% and specificity of 8.9% at the threshold of 0.8 mmol/L.Because of the large TE , the Freestyle Optium Neo H and TaiDoc-TD4235 meters should not be used to quantify blood β-OHB concentrations in sheep.Background: Accurate and rapid measurement of blood β-hydroxybutyrate (β-OHB) concentrations is critical to identify hyperketonemia in sheep. Objective: The objective of this study was to test the performance of Freestyle Optium Neo H and TaiDoc-TD4235 point-of-care (POC) devices for measuring blood β-OHB concentrations in sheep. Methods: Venous blood samples from 105 sheep were evaluated with Freestyle Optium Neo H and TaiDoc-TD4235 meters. A reference approach was the laboratory measurement of serum BHB concentrations using the Randox D-3 Hydroxybutyrate reagent kit. Data were analyzed using Passing- Bablok regression and Bland–Altman analysis. The diagnostic accuracy was assessed using serum β-OHB concentration at a cut-off value of 0.8 mmol/L. Results: Passing-Bablok regression analysis revealed an intercept of 0.066 (CI95: 0.018–0.125) and slope of 1.242 (CI95: 1.136–1.342) for Freestyle Optium Neo H. Intercept and slope values for the TaiDoc-TD4235 meter were 0.625 (CI95: 0.539–0.724) and 1.265 (CI95: 1.044–1.497), respectively. The Bland–Altman plot revealed a bias of 0.224 mmol/L and 0.737 mmol/L for Freestyle Optium Neo H and TaiDoc-TD4235 meters, respectively, compared with the reference method. Observed Total Error (TEobs) was 56.7% for the Freestyle Optium Neo H and 168.7% for the Taidoc-TD4235. The sensitivity and specificity for the Freestyle Optium Neo H were 100% and 80.9%, respectively, at the threshold of 0.8 mmol/L. The Taidoc-TD4235 demonstrated a sensitivity of 93.3% and specificity of 8.9% at the threshold of 0.8 mmol/L. Conclusions: Because of the large TEobs, the Freestyle Optium Neo H and TaiDoc-TD4235 meters should not be used to quantify blood β-OHB concentrations in sheep.falsebiasewepoint-of-care devicepregnancy toxemiasensitivityspecificitybias | ewe | point-of-care device | pregnancy toxemia | sensitivity | specificityJournal Article10.1111/vcp.1322310.1111/vcp.132232-s2.0-85149377546WOS:000941885600001368596021939-165X