Browsing by Author "Yildirim, A."

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    Web of Science
    Phylogenetic characterization of Cochroaches (Insecta: Blattaria) in Türkiye and determination of their vector potential for medically important parasites
    (2024.01.01) Cevahir, F.; Düzlü, Ö.; Atelge, M.; Yildirim, A.
    This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I (mt-COI), mt-COII, and internal transcribed spacer-2 (ITS-2) gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism (RFLP) was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species (n=220) collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica (n=105), Blatta orientalis (n=86), and Periplaneta americana (n=29). A total of 13 haplotypes were detected and maximum likelihood (ML) analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 (79.3%) belonged to helminth species and 12 (20.7%) to protozoan species. The results showed that B. germanica (58.6%) had the highest prevalence, followed by Bl. orientalis (32.8%) and P. americana (8.6%). The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites.
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    Web of Science
    Phylogenetic characterization of two common sandflies, Phlebotomus major and P. kandelakii, in Inebolu District of the West Black Sea Region, Türkiye based on mitochondrial gene sequence analysis
    (2024.01.01) Karademir, G.K.; Atelge, M.; Yetismis, K.; Yetismis, G.; Uslu, S.; Çiloglu, A.; Önder, Z.; Özbel, Y.; Pekmezci, G.Z.; Yildirim, A.; Düzlü, Ö.; Toz, S.; Pekmezci, D.; Inci, A.

    Phlebotomus major and P. kandelakii are members of the Larroussius subgenus, which includes important vector sand fly species. Most members of the subgenus Larroussius have the ability to transmit Leishmania infantum, , the causative agent of visceral leishmaniasis. Here, we investigated the genetic diversity within each species collected from the West Black Sea Region of T & uuml;rkiye using mitochondrial DNA markers, specifically cytochrome oxidase I (COI) and cytochrome b gene sequences (Cytb). A total of 1889 sand fly specimens were collected from the study area in June 2021 and August 2022; 1596 (84.49%) were identified as P. major sensu lato, and 253 (13.40%) were identified as P. kandelakii. . Nine and four haplotypes of P. major were determined in the study area based on COI and Cytb sequences, respectively. Analysis of the phylogenetic datasets generated from our isolates and published isolates in GenBank revealed high haplotype diversities within P. major (COI = 0.933, Cytb = 0.714). For P. kandelakii, , we detected four and three haplotypes within the COI and Cytb sequences, and the haplotype diversities were also high in the datasets, including our isolates and published isolates in GenBank (COI = 0.978, Cytb = 1.000). Pairwise mean genetic distances calculated from the COI and Cytb datasets were 0.4% and 1.4% for P. major and 1.0% and 0.2% for P. kandelakii, , respectively, suggesting the absence of cryptic species. Phylogenetic analyses revealed three and two major clusters of the Larroussius subgenus in the COI and Cytb datasets, respectively. Our study contributes to molecular information for P. major and P. kandelakii distributed in T & uuml;rkiye and provides valuable insights into the phylogenetic relationships among species within the subgenus Larroussius.p>