Browsing by Author "Uysal S."

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    New perspectives into the chemical characterization of Sida acuta Burm. f. extracts with respect to its anti-cancer, antioxidant and enzyme inhibitory effects
    (2021-06-01) Uysal S.; Gevrenova R.; Sinan K.I.; Bayarslan A.U.; Altunoglu Y.C.; Zheleva-Dimitrova D.; Ak G.; Baloglu M.C.; Etienne O.K.; Lobine D.; Mahomoodally M.F.; Zengin G.
    The chemical components, in vitro antioxidant capacities and enzyme inhibitory effects of dichloromethane (DCM), ethyl acetate (EA), methanol (MeOH) and water extracts of the medicinal plant, Sida acuta Burm. f. were evaluated. The individual phenolic components were assessed via ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS). In terms of chemical composition, phenolics (hydroxybenzoic and hydroxycinnamic acids) and flavonoids were identified as main groups. The antioxidant capacities were evaluated using a panoply of cell-free bioassays and the enzymatic inhibitory potentials against key enzymes involved in human diseases were also determined. The water extract exhibited the strongest scavenging abilities on DPPH (IC50: 1.05 mg/mL) and ABTS (IC50: 1.02 mg /mL). The best cholinesterase inhibitory abilities were observed with the methanol extract (IC50: 0.51 and 0.52 mg/mL for AChE and BChE, respectively), while DCM exhibited the strongest α-amylase (IC50: 1.21 mg/mL) and α-glucosidase (IC50: 0.62 mg/mL) inhibitory effects. The anti-cancer effects of methanol and water extracts were tested on human breast cancer cells, MDA-MB-231, and the methanol extract showed the best anti-cancer effect with an IC50 value of 102.4 μg/mL. In conclusion, the experimental data have demonstrated promising pharmacological activities of S. acuta extracts obtained using different solvents, thereby providing a scientific basis for the validation of the traditional medicinal uses of this plant.
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    Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.
    (2018-01-01) Uysal S.; Ugurlu A.; Zengin G.; Baloglu M.C.; Altunoglu Y.C.; Mollica A.; Custodio L.; Neng N.R.; Nogueira J.M.F.; Mahomoodally M.F.
    Bidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silico methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest α-amylase inhibitory activity, while the best α-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to α-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases.